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Citations for Bio-Rad :
3601 - 3650 of 6467 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The radio-labelled proteins were then separated with SDS-PAGE on a 4-12% Criterion XT Bis-Tris gels (Bio-Rad, Hercules, CA, USA) in MES buffer (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... The proteins in the lysates were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis using gradient (4–20 %) PAGE gels (Bio-Rad, Mini-PROTEAN TGX), with a molecular mass marker (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... the indicated ESA supernatant was boiled in SDS-PAGE sample buffer and 3 μg of each sample was run with a 4-15% TGX gradient gel (Bio-Rad cat. no. 4561086), then transferred to a nitrocellulose membrane ...
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bioRxiv - Genetics 2022Quote: ... 30μg protein for iPSC-derived optic cups or 15μg for iPSC-LESCs were loaded onto 4-15% Mini-PROTEAN ® TGX ™ gels (BioRad Inc., CA, USA) and transferred to an Immun-Blot™ PVDF membrane using a Trans-Blot® SD semi-dry transfer cell (BioRad Inc. ...
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bioRxiv - Microbiology 2024Quote: ... A 40 µg of protein of CFS samples and 10 ng of purified rGdhA were resolved in a 4-20% SDS-PAGE (Bio-Rad, Hercules, CA; # 4561094) and immunoblotted with affinity purified anti-rabbit rGdhA antibody (0.3 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... at 95°C for 10 mins before being loaded into 4-20% Mini-PROTEAN® TGXTM Precast Protein Gels (15 wells, 15uL; Bio-Rad, CA, USA) and subject to electrophoresis on a Bio-Rad Mini-PROTEAN® Tetra Cell system (180 V ...
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bioRxiv - Neuroscience 2024Quote: ... 10 µg of each lysate sample were separated though sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-20% Criterion precast gels (Bio-Rad Cat #456-1095). Proteins were blotted onto Polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: Protein samples were heated at 70°C for 10 minutes and separated by gel electrophoresis using the Bio-Rad Mini-Protean system with 4–20% gradient gels (Bio-Rad, Cat# 456-1094). After electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... 250 μL of the mixed cell suspension was chilled on ice for 5-10 minutes in a 4-mm wide cuvette compatible with the Gene Pulser®/MicroPulser™ (BioRad, Hercules, CA). The cell mixture was electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... was chilled on ice for five to ten minutes in a 4-mm wide cuvette compatible with the Gene Pulser®/MicroPulser™ (BioRad, Hercules, CA). The cell-DNA mixture was electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... and samples were boiled for 5min at 95°C and then loaded on 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad #4568093). Gel was run at 100V for 90 minutes and proteins were transferred to a nitrocellulose membrane using the Bio-Rad Transblot Turbo system ...
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bioRxiv - Immunology 2024Quote: ... Proteins were denatured at 94°C for 5 min before separation on SDS-PAGE (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels were used (Bio-Rad Laboratories)) ...
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bioRxiv - Genomics 2024Quote: ... CD45-positive and CD3-positive cells were centrifuged at 400 x g for 7 minutes at 4 ºC and counted with a TC20™ Automated Cell Counter (Bio-Rad Laboratories, S.A), before proceeding to single cell RNA sequencing.
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bioRxiv - Neuroscience 2024Quote: ... 30µg of protein homogenates were separated by SDS-PAGE on a 4-12% gradient Bis-Tris gel (Bio-Rad, Hercules, CA, Criterion XT 3450125) in XT MES buffer (Bio-Rad ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Whole cell lysates containing SDS buffer were homogenized with a 27-G syringe and resolved on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 6/10/12% home-made polyacrylamide gels and transferred to nitrocellulose membranes (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently subjected to SDS-polyacrylamide gel electrophoresis (PAGE) on a 4–15% Mini Protean TGX precast gel (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (10 µg of protein per well) were separated on SDS-polyacrylamide gels (CriterionTM TGXTM Precast Gels, 4-15%; Bio-Rad, Hercules, CA, USA) using a Laemmli buffer system and then semidry transferred to nitrocellulose membranes by a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: Thirty micrograms of protein from the cell lysate in RIPA buffer was separated by reducing SDS-PAGE on a 4-15% polyacrylamide gel (Bio-Rad, Hercules, California, USA). The protein was transferred to a nitrocellulose membrane using a semi-dry transfer method [36] ...
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bioRxiv - Neuroscience 2024Quote: ... Samples containing 15 µg of total proteins were run in duplicate onto 4-15% Criterion™ TGX Stain-free™ precast gels or 12% Criterion™ TGX precast gels (Bio-Rad) and transferred into nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... a mixture of 250 μL of this cell suspension and 1000 ng of a vector plasmid digested with XbaI and KpnI enzymes was chilled on ice for 5–10 minutes within a 4-mm width cuvette compatible with the Gene Pulser®/MicroPulser™ system (BioRad, Hercules, CA). The cell-DNA mixture was then electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Biophysics 2024Quote: ... before boiling the sampling in a reducing sample buffer and separation on a 4-15 % mini-PROTEAN Precast SDS-PAGE (Bio-Rad, Cat No 4561085). Transfer to PVDF membrane was performed using a Trans-Blot pack (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 16,100 rcf for 15 minutes at 4 °C and protein concentration was quantified using the DC™ Protein Assay kit (Bio-Rad, Hercules, CA).
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bioRxiv - Biochemistry 2024Quote: Cell lysates containing 1X SDS buffer were homogenized with 27-G syringe and whole lysates were run on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 6/10/12% home-made polyacrylamide gels before being transferred to PVDF membranes (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... and 15ug of denatured protein lysate was separated on a 4-15% Mini-Protean TGX Precast Protein Gel (Cat# 4561086, Bio-Rad Laboratories, Inc. USA). After transfer to 0.2 um PVDF membrane using TransBlot Turbo Transfer system (Cat# 1704272 ...
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bioRxiv - Cell Biology 2024Quote: ... and boiled for 5 min before loading into a 12% or 4-20% acrylamide pre-cast SDS- PAGE gels (Bio-Rad, 4561045 or 4568094) for electrophoresis ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216, Anti-FLAG; 1:2000 dilution, Sigma-Aldrich #F1804, anti-β-Tubulin; 1:1000 dilution, Bio-Rad #12004166). Then membranes were washed with 1X TBST 3 times (10mins each wash ...
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bioRxiv - Plant Biology 2023Quote: ... followed by addition of primary antibodies at 4 °C overnight with rotation (mouse anti-ϕ3-actin [Proteintech, 66009-1-Ig], 1:1000, and rat anti-χξ-tubulin [YOL1/34, MCA78G, Bio-Rad], 1:1000). The specimen was washed three times with PBST and incubated with secondary antibodies (anti-mouse ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216, Anti-FLAG; 1:2000 dilution, Sigma-Aldrich #F1804, anti-β-Tubulin; 1:1000 dilution, Bio-Rad #12004166). Then membranes were washed with 1X TBST 3 times (5 mins each wash ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were anti-mouse (Bio-Rad, #170-6516, dilution 1:1000 or 1:2000) and anti-rabbit (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... 0.05% SDS) and a semi dry transfer cell (Bio-Rad, 1 mA/cm2, 1 h). Membrane was blocked with protein free blocking solution T20 (Pierce ...
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bioRxiv - Immunology 2020Quote: ... A total of 50μg protein was diluted 1:1 with 2x Laemmli buffer (Bio-Rad), resolved by SDS-PAGE and transferred into PVDF membranes ...
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bioRxiv - Bioengineering 2021Quote: ... samples were mixed 1:1 with 2x Laemmli sample buffer with 2-mercaptoethanol (Bio-Rad) and boiled for 10min ...
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bioRxiv - Biochemistry 2021Quote: Purified protein was mixed in 1:1 ratio with 2X laemmli loading dye (Bio-rad) prepared according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1× iTaq Universal SYBR Green Supermix or 1× iTaq Universal Probe Supermix (Bio-Rad, USA), 300 nM each of forward primer (late RT product ...
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bioRxiv - Physiology 2021Quote: ... Sections were incubated with F4/80 antibody (Clone Cl:A3-1, Bio-Rad, MCA497R, 1:100) followed by biotinylated antibody (Vector ...
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bioRxiv - Physiology 2023Quote: ... prepared at a 1:1 ratio and acquired using the ChemiDoc MP System (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were mixed 1:1 with 2X Laemmli Sample Buffer (Bio-Rad, Hercules, CA) (5% β-mercaptoethanol ...
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bioRxiv - Immunology 2024Quote: Samples were prepared in 1 x Laemmli buffer with or without 1 x dithiothreitol (Biorad). Samples were then heated for 10 min at 95 °C and loaded onto a precast NuPAGE™ 4-12 % Bis-Tris polyacrylamide gel in NuPAGE™ MES SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with Laemmli Sample Buffer (1610737, BIO-RAD, Hercules, CA) and heated to 80°C for 30 min.
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with the HRP-conjugated secondary antibodies (1:2500-1:10000, Bio-Rad) for 1h at RT ...
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bioRxiv - Genetics 2022Quote: Samples taken from prior assays were mixed 1:1 with Laemmli Sample Buffer (Bio-Rad) with 10% 2-mercaptoethanol and vortexed to ensure adequate mixing ...
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bioRxiv - Systems Biology 2024Quote: ... Dried pellets were dissolved in 25 μl 1% SDS buffer (1% SDS (Bio-Rad 1610418), 50 mM HEPES (Gibco 15630-080 ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were then diluted 1:1 in 2x Laemmli sample buffer (Bio-Rad, 1610737) and boiled for 5 minutes at 95°C ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibodies for 1 hour at room temperature (anti-rabbit or anti-mouse, 1:10.000, Bio-Rad, anti-goat, 1:7500, Pierce). Antibody binding was detected by chemiluminescence(Clarity kit ...
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bioRxiv - Cell Biology 2020Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels and the Trans-Blot® Turbo™ transfer system (Bio-Rad). The following antibodies ...
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bioRxiv - Cell Biology 2020Quote: Equal amounts of protein were loaded onto 12% (v/v) resolving SDS-PAGE gels with 4% (v/v) polyacrylamide stacking gels using Precision Plus Protein™ dual colour standards (Bio-Rad, Hercules, CA, USA). The gels were run in a Mini-Protean® Tetra Cell system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Purified proteins were eluted in SDS-PAGE sample loading buffer and separated in 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad# 4561094, 4561096) either for western blot (WB ...
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bioRxiv - Biochemistry 2021Quote: Cell lysates were prepared using RIPA buffer (see lysis and click chemistry methods below) and run on SDS Tris-Glycine gels (Precast 4 - 20% Bio-Rad, or hand-casted 20%). Gels were transferred to low-background PVDF membranes using the BioRad TransBlot Turbo system and blocked with 5% milk powder in TBS + 0.1% Tween 20 (TBST ...
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bioRxiv - Microbiology 2020Quote: Twenty-fold concentrated viral stocks or purified rgp120 were separated by SDS-PAGE under reducing conditions using a 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad Laboratory Inc. Hercules, CA), and then transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...