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Citations for Bio-Rad :
251 - 300 of 5400 citations for 4 Methoxy 6 methyl 6 phenyl 5H pyran 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was separated by non-reducing SDS-PAGE (6% acrylamide gel) with Precision Plus Protein Dual Color Standards (Bio-Rad) and transferred to nitrocellulose membrane overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs isolated from two donors were infected with GFP-EBV for 28 days in 6-well plates and subsequent B-cells blasts were photographed using using a ZOE Fluorescent Cell Imager (BIO-RAD). Using similar strategy ...
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bioRxiv - Genomics 2022Quote: ... and 20 ug of each sample were loaded on a 6% or 10% SDS-PAGE gel and transferred to a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz ...
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bioRxiv - Cell Biology 2024Quote: ... The maleamide reaction was quenched with 5 mM DTT and unreacted Halo Ligand and Quencher were removed using two Bio-Spin 6 (Tris) columns (Bio-rad).
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bioRxiv - Biochemistry 2024Quote: ... or a combination of both was added for 6 hours prior to imaging with either a ZOE Fluorescent Cell Imager (Bio-Rad) or EVOS FL Cell Imaging System (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... The gel obtained was imaged using the Silver Stain setting on Image Gel 6 on Gel Doc EZ Imager (Bio-Rad).
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bioRxiv - Evolutionary Biology 2024Quote: Protein samples were buffer exchanged into 200mM ammonium acetate using either a centrifugal buffer exchange device (Micro Bio-Spin P-6 Gel, Bio-Rad) or a dialysis device (Slide-A-Lyzer MINI Dialysis Unit ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative real-time PCR was performed on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System using iTaq Universal SYBR Green Supermix (Bio-Rad). 18S rRNA was used as an internal control ...
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bioRxiv - Cell Biology 2021Quote: Cells lysates were separated on 4-2% gradient SDS-polyacrylamide gels (Biorad), transferred to polyvinylidene fluoride (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... Quantity One (Bio-Rad) was used to analyze Northern blot results ...
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bioRxiv - Biochemistry 2021Quote: ... and Quantity One (Biorad) were used to analyze Western blots and Northern blots ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane homogenates (50 μg) were separated on 6% SDS-PAGE gels and transferred onto presoaked PVDF membranes (Bio-Rad, Pleasanton, CA, USA). Blots were then blocked with 10% non-fat milk in PBST (100 nM phosphate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were run on 6% SDS-PAGE gels and blotted onto nitrocellulose using a semi-dry Trans-Blot (Bio-Rad, 1703940). Blots were incubated overnight at 4°C with a rabbit anti-myosin 18A polyclonal antibody that recognizes the C-terminal 18 residues of both myosin 18Aα and 18Aβ (Bruun et al. ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) in D2O using Micro Bio-Spin® Columns with Bio-Gel® P-6 (Bio-Rad Laboratories, Hercules, CA, USA). To avoid aggregation ...
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bioRxiv - Bioengineering 2021Quote: ... were desalted and buffer exchanged into 10 mM ammonium acetate using a spin column (Micro Bio-Spin™ P-6 Gel Columns, Bio-Rad). Octylamine was added to aliquots of NCB solution at a concentration of 0.1% (v/v) ...
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bioRxiv - Molecular Biology 2022Quote: ... The bombardment was performed (a distance of 6 cm using 1350 psi rupture disks) with a PDS-1000/He Biolistic Particle Delivery system (Bio-Rad, USA). The antibiotic concentration was determined after a sensitivity test ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was homogenized in 10 mM sodium acetate buffer (500 μL, pH 6.5) and filtered through Bio-spin 6 column (Bio-Rad, Hercules, CA) prewashed with 10 mM sodium acetate buffer (pH 6.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Purification of the desalted bacteriocin protein was performed using an Uno S-6 prepacked monolith cation exchange column (12×55 mm, Bio-Rad, USA) on a FPLC system (BioLogic DuoFlow System ...
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bioRxiv - Biochemistry 2020Quote: ... the desalted protein fraction was purified using the Uno S6 prepacked monolith cation exchange column (12 × 53 mm, 6 mL, Bio-Rad, USA). The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (1 ml) (Healthcare Life Sciences) were loaded into disposable Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The qPCR was performed with a QuantStudio 6 Flex system equipped with a 384-well block and the SspAdvanced Unversal SYBR® Green Supermix (#1725272, BioRad, Switzerland) under the following conditions ...
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bioRxiv - Microbiology 2024Quote: The tRNA modified product from MenT3 in vitro reactions underwent the following steps: it was initially purified using Bio-Spin® 6 Columns (Bio-Rad), followed by incubation with a putative exoribonuclease (10 µM ...
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bioRxiv - Biochemistry 2023Quote: ... followed by heat-inactivation at 75°C for 5 min and desalting using Micro Bio-Spin P-6 columns (Bio-Rad, 7326221). Dephosphorylated guide RNAs were then radiolabeled using T4 Polynucleotide Kinase (0.33 U/µL ...
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bioRxiv - Biochemistry 2022Quote: ... SDS-PAGE and size exclusion chromatography (SEC) using a Superose® 6 Increase 10/300 GL column (Cytiva) and an NGC Quest 10 Plus System (Bio-Rad) showed that both wild-type and variant proteins were pure and monodisperse (Figure 2-figure supplement 1).
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bioRxiv - Plant Biology 2023Quote: ... and proteins were separated in SDS/urea–PAGE (15% polyacrylamide, 6 M urea) (Laemmli, 1970) using mini-protean apparatus (Bio-Rad USA). These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA) ...
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bioRxiv - Immunology 2024Quote: ... were covalently coupled to carboxyl-functionalized fluorescent magnetic beads (1-3µg/1.25*10^6 beads) (Luminex Corp. MagPlex® Microspheres; Bio-Rad; Temse, Belgium) employing the BioPlex amine coupling kit (Ref. ...
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bioRxiv - Developmental Biology 2024Quote: ... and electroporated (square wave, 6 pulses, 30 V, 3 ms duration, 100 ms interval) using a gene Pulser XCell electroporator (Bio-Rad, 1652660). Zygotes were recovered and transferred to fresh KSOM drops and cultured overnight ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 6% milk in PBS and subsequently stained with mouse anti-V5 (clone SV5-Pk1, BioRad, 1:10 000) and goat anti-mouse (1:100 000 ...
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bioRxiv - Biophysics 2024Quote: ... The reaction volume was brought up to 50 μL by addition of MilliQ water and run through a Micro Bio-Spin™ 6 column (Biorad, 7326221) as per manufacturer instructions to purify radiolabelled oligonucleotide from free γ 32P ATP ...
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bioRxiv - Biochemistry 2024Quote: ... Excess NEM or TCEP were removed by size exclusion chromatography with a 6 kDa cut-off spin column (Bio-Rad, UK, #7326222). After determining the protein content via a Bradford assay ...
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bioRxiv - Developmental Biology 2023Quote: ... one flash-frozen for DNA sequencing studies and one lysed in Purezol (Biorad) to obtain RNA for RT-qPCR studies.
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bioRxiv - Neuroscience 2022Quote: ... anti-Ly-6B.2 (1:100, clone 7/4, BioRad, raised in rat) and anti-Nur77 (NR4A1 ...
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bioRxiv - Biophysics 2021Quote: ... The mixture was diluted to ~30 mL with sample buffer and stirred at 4°C for 30 min prior removal of C12E8 by adding 4 g of Bio-Beads SM-2 (Bio-Rad) in stages of 0.5 ...
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bioRxiv - Biochemistry 2021Quote: ... and their respective complexes with Hap40 protein were buffer-exchanged into 150 mM aqueous ammonium acetate (pH=7.5) by using P-6 Bio-Spin gel filtration columns (Bio-rad, Veenendaal, the Netherlands). The protein’s resulting concentration was estimated to be ~2-5 μM before native MS analysis ...
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bioRxiv - Microbiology 2020Quote: ... The precipitated proteins were then dissolved in 6 M urea/50 mM ammonium bicarbonate buffer (pH 8) for protein quantitation using DC™ protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Genetics 2022Quote: ... The DNA was diluted to 6 ng/µl for copy number screening and qPCR was performed using SsoAdvanced™ Universal SYBR® Green Supermix (Biorad, 1725272) and 1µM primer mix to amplify the target within and flanking the deleted region ...
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bioRxiv - Physiology 2023Quote: ... 18 and 21 months of age and were subjected to IEF using a 24 cm immobiline DryStrip (pH 6-11) (BIO-RAD, Hercules, CA), followed by SDS-PAGE using pre-casted 10-16% gradient polyacrylamide gels (BIO-RAD ...
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bioRxiv - Molecular Biology 2022Quote: ... was performed for 23 h at 14°C with an initial switch time of 60 s and a final switch time of 120 s at 6 V/cm and an included angle of 120° as described previously (Bio-Rad CHEF Mapper) [35] ...
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bioRxiv - Immunology 2024Quote: ... ACPA IgG levels were quantified based on an in-house standard of pooled RA patient plasma using the Microplate manager software MPM-6 (Bio-Rad Laboratories, Inc.). Unspecific reactivity to the peptide backbone was assessed by applying CArgP2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates (10-100 μg proteins/lane) were loaded to and separated on 6%-15% SDS-PAGE gels and electrophoretically transferred to PVDF membrane (1620177, Bio-Rad, Hercules, CA). The membranes were blocked in 4% BSA/PBST (0.1% Tween-20 ...
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bioRxiv - Biophysics 2023Quote: Proteins samples were kept on ice and buffer exchanged into 200 mM ammonium acetate buffer at pH 7 using Micro Bio-spin columns with either a 6 or 30 kDa cutoff (Bio-Rad, Hercules, CA). Buffer exchanged samples were then diluted to a working concentration of 10 μM before analysis ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quantity One Software (Bio-Rad) was used to analyze the results.
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bioRxiv - Molecular Biology 2024Quote: ... Quantity One software (Bio-Rad) was used for image acquisition and data analysis ...
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bioRxiv - Biophysics 2022Quote: ... 1 mg of post-thrombin digested claudin-4 in DDM was treated with 4 mg of amphipol (1:4 w/w) for 2 hours before removing detergent via addition of 400 mg SM-2 biobeads (Bio-Rad). Excess cCpE was added to each claudin-4 sample at a molar ratio of 1:1.5 ...
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bioRxiv - Immunology 2022Quote: ... for 2 hours at 4 °C using Mini-trans blot wet tank transfer (BioRad). After transfer ...
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bioRxiv - Biophysics 2021Quote: ... and 3.6 μL ddH2O in a 10 μL total reaction volume using Bio-Rad CFX Connect™ Real-Time PCR System (Bio-Rad, CA, USA). The 2-ΔΔCt method was used for the quantitative analysis ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was extracted from several colonies of a 6-week-old subculture on Coletsos culture medium using the InstaGene matrix following the manufacturer’s instructions (Bio-Rad, Marnes-la-Coquette, France). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting transcripts were treated with DNase for 20 min at room temperature and then isolated using Micro Bio-Spin™ 6 Columns (BioRad, Cat.# 732-6221). 0.75 µg of RNA transcripts were mixed with 2X formamide buffer (95% deionized formamide ...
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bioRxiv - Biochemistry 2020Quote: ... the SL2-6 construct was subsequently size purified using a denaturing polyacrylamide gel (7M Urea, 1x TBE, hand-poured in Bio-Rad Criterion midi cassettes), loaded in 80% formamide ...
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bioRxiv - Immunology 2021Quote: ... IL-6, TNF-α, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, IL-1RA, G-CSF and Eotaxin; Bio-Rad Laboratories Inc.) following the manufacturer’s instructions ...