Labshake search
Citations for Bio-Rad :
851 - 900 of 2983 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... 5 ng of cDNA was then subjected to qPCR analysis using iQ SYBR Green super-mix reagent (BioRad). Gene expression levels were normalized to a housekeeping gene (TATA-binding protein or HPRT) ...
-
bioRxiv - Physiology 2024Quote: ... Protein (5-10 µg) was separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad, USA) in 26-well 4–15% Criterion™ TGX Stain-Free™ Protein Gels (Bio-Rad ...
-
bioRxiv - Molecular Biology 2024Quote: ... The formazan crystals formed by the cells were dissolved by adding 100 µL of 5% SDS (Bio-Rad) in 1N HCl (Thermo Fisher ...
-
bioRxiv - Biochemistry 2024Quote: ... Integrity of the annealed oligonucleotides was determined by application to Mini-PROTEAN native 5% polyacrylamide TBE gel (Biorad), staining with SYBR safe DNA gel stain (Thermo Fischer Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked at room temperature for 1 hour in 5% nonfat dry milk (blocking grade, Bio-Rad) dissolved in Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
-
bioRxiv - Biochemistry 2024Quote: ... Pooled peak fractions were then loaded onto a 5 ml Bio-Scale Mini CHT Type I Cartridge (Biorad) in 10 mM NaH2PO4 Buffer and eluted sequentially with 2CV 40 mM NaH2PO4 Buffer ...
-
bioRxiv - Genetics 2024Quote: ... Electroporation was performed at 2.5 kV for 5 ms in a MicroPulser electroporator (Bio-Rad, Hercules, California, USA) using 0.2 cm cuvettes ...
-
bioRxiv - Cancer Biology 2024Quote: ... at 95 degrees Celsius for 5 minutes before loading onto 10% Mini-PROTEAN TGX precast protein gel (BioRad). PageRuler Plus Pre-stained Protein Ladder (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 5 µg total protein was loaded on each lane of a Mini Protean TGX Gel (BioRad, #4561093) together with a standard (WesternC ...
-
bioRxiv - Biochemistry 2024Quote: ... boiled for 5 min and then resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
-
bioRxiv - Biophysics 2020Quote: ... before being transferred to Whatman 3 MM paper and dried (50°C, 2 hours) using a gel dryer (Model 583, BioRad) attached to a vacuum pump ...
-
bioRxiv - Microbiology 2020Quote: ... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
-
bioRxiv - Microbiology 2024Quote: ... The membrane was washed 3 times with PBS-T and developed with ECL (Biorad170-5060) for 2 min and imaged on ChemiDocTMMP (Biorad).
-
bioRxiv - Molecular Biology 2024Quote: ... The absorbances of in 5-Mercapto-2-nitrobenzoic acid (TNB) in the samples was measured at 415 nm using a BioRad iMark microplate reader (Bio-Rad Laboratories, TX, USA).
-
bioRxiv - Molecular Biology 2020Quote: ... samples were boiled for 5 min and equal volumes were loaded onto a 4-15% gradient gel (Bio-Rad) for SDS-PAGE ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Each female was macerated in a 50 μl 5% Chelex (Chelex 100 Resin, Bio-Rad Laboratories, Hercules, CA, USA) solution and 1μl proteinase K ...
-
bioRxiv - Developmental Biology 2021Quote: ... The PVDF membrane was blocked for 30 minutes at RT using 5% Blotting-Grade Blocker (170-6404, BIO-RAD)/Tris buffered saline with Tween 20 (TBST ...
-
bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were incubated in 5% BLOT-QuickBlocker as follows: goat anti-mouse HRP conjugate (BioRad, 1706516; 1:5000), donkey anti-rabbit HRP conjugate (Amersham ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5 μl of lysate was used for protein determination by the DC Protein Assay (Bio-Rad Laboratories, Hercules, CA).
-
bioRxiv - Genomics 2022Quote: ... The gel was then soaked into the staining solution for 5 minutes and then directly scanned by Bio-rad ChemiDoc™ Touch Imaging System and the gel image was analyzed.
-
bioRxiv - Developmental Biology 2020Quote: ... the membrane was blocked overnight at 4°C using 5% non-fat dry milk (Bio-Rad, catalog 170-6404) in Tris-buffered saline pH 7.3 ...
-
bioRxiv - Microbiology 2020Quote: ... or by hand at 5 μl per well into 96-well hard shell low profile PCR plates (Bio-rad) for bulk RNA sequencing ...
-
bioRxiv - Bioengineering 2020Quote: The cDNA was diluted 1/5 with dH2O and 2.5 µL were used for qPCR analysis (10 µL total volume; BioRad, iTaq Universal SYBR Green Supermix ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were heated at 55° C for 5 minutes and 30 µg of protein per sample was run on Tris-Tricine 16.5% precast polyacrylamide gels (BioRad) for Aβ or 10% Tris-Glycine (TGX ...
-
bioRxiv - Neuroscience 2020Quote: ... 15 μg total RNA from HEK293 cells were separated on 5% Criterion™ TBE polyacrylamide gels (#3450048, Bio-Rad) and transferred to positively charged nylon membrane ...
-
bioRxiv - Physiology 2021Quote: ... Samples (5 μL) were then loaded on precast gradients (4-15%) SDS-polyacrylamide gels in duplicate (Bio-Rad Laboratories) and subjected to electrophoresis at 180 V for 40 minutes using pre-made 1x SDS-PAGE running buffer (Ameresco) ...
-
bioRxiv - Cell Biology 2021Quote: ... Nuclear (5 µg total protein) and cytoplasmic (10 µg total protein) fractions were boiled in Laemmli buffer (Bio-Rad), run on Criterion 4-20% Gels (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... 40 µL of supernatant containing 5 µg of proteins were mixed to 36 μL Laemmli Buffer 1x (Bio-Rad) and 4 µL β-mercaptoethanol BioUltra (Sigma ...
-
bioRxiv - Synthetic Biology 2021Quote: ... the membrane was placed in Pierce chemiluminescence substrates for 5 minutes and imaged on film using ChemiDoc (Bio-Rad).
-
bioRxiv - Neuroscience 2020Quote: ... membranes were washed in a solution of TBS+0.01 % Tween-20 (TBS-T) and incubated in 5 % non-fat milk (Biorad) blocking solution for 2 h ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 to 50 µg of DNA was electroporated (375 V, 1,000 µF, 750 Ω; GenePulser Xcell; BioRad, Hercules, CA) into trophozoites ...
-
bioRxiv - Cell Biology 2020Quote: ... Standard qPCR reactions with 5 ng cDNA per reaction were performed with iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX Connect Real-Time PCR Detection System ...
-
bioRxiv - Cancer Biology 2022Quote: ... samples were heated for 5’ at 95°C in Lämmli buffer and protein lysates were resolved on polyacrylamide Mini-PROTEAN TGX gels (BioRad) and transferred onto nitrocellulose membrane by wet-transfer ...
-
bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was performed on 5 ng/μL cDNA using iTaq Universal SYBR Green Supermix (Bio-rad, 64361172) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... Onion epidermal cells were bombarded with 5 μg of each DNA construct using a helium biolistic gun (BIO-RAD). Treated epidermal cells were kept in the dark at room temperature for 16 h before analysis by confocal microscopy (Confocal Olympus FV 1000) ...
-
bioRxiv - Microbiology 2020Quote: ... purified on Ni-NTA resin (G-Biosciences, #786-940 in 5 ml Bio-Scale™ Mini Cartridges, BioRad #7324661), then dialyzed ...
-
bioRxiv - Neuroscience 2020Quote: ... and blocked with 5% (w/v) non-fat milk tris-buffered saline (TBS) with 0.1% Tween-20 (Bio-Rad) for 1 hour at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... pH 8.3) running buffer by loading 5 μg sample onto 4-20% Mini-PROTEAN® TGXTM gels (Bio-Rad) with iBrightTM Prestained Protein Ladder (#LC5615 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Genomic DNA was obtained from single adults via incubation in 100µL of 5% w/v Chelex beads (BioRad Inc.) at 55°C ...
-
bioRxiv - Microbiology 2020Quote: ... 20 mL blocking buffer supplemented with 5 μL of horseradish peroxidase (HRP) conjugated rabbit anti-mouse secondary antibody (BioRad) was added and incubated for 2 hours at room temperature with agitation ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and 15μg protein in 5x Laemmli buffer with 5% b-mercaptoethanol was loaded on Mini-PROTEAN TGX Precast Gels (BioRad). Gel lanes were cut into three sections for peptide extraction ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were washed 3 times with PBS and then blocked for 1 hour at room temperature with DPBS with 0.4% Triton X-100 and 5% non-fat dry milk (BioRad). Cells were stained with primary and secondary antibodies (Table S3 ...
-
bioRxiv - Cell Biology 2020Quote: Immunoprecipitated proteins were resuspended in SDS-PAGE sample buffer and heated 5 minutes at 95°C before loading onto a 7.5% acrylamide mini-Protean TGX gel (BioRad). After 7 minutes of electrophoresis at 100 V the gel was stained with Imperial Protein stain (Thermo ...
-
bioRxiv - Systems Biology 2020Quote: ... A total of 800 µl of cell suspension mixed with 10 µg of pDM304_Red-FL2 in a 4-mm-width cuvette was subjected to electroporation (two 5 sec separated pulses with 1.0 kV and a 1.0 msec time constant) using a MicroPulser (Bio-Rad). These cells were plated on 4–6 pieces of 90-mm plastic dishes with HL5 medium and incubated at 22°C for 18 hours under the non-selective conditions and then cultured in the presence of 10 µg/ml G418 (Wako) ...
-
bioRxiv - Genomics 2021Quote: ... 1 μL diluted cDNA in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each sample we conducted three technical replicates and used their mean as the data point ...
-
bioRxiv - Genomics 2021Quote: ... They were then boiled for 5 minutes at 99°C after the addition of Laemmli buffer (Cat. #1410737, Biorad). Proteins were separated by SDS-PAGE and transferred to a membrane with a Trans Blot TURBO (Biorad) ...
-
bioRxiv - Biochemistry 2021Quote: ... 5 μM of proteins and 3x of SYPRO™ Orange were pipetted into a 96-well PCR plate (BioRad, MicroAmp Fast 96-Well Reaction plate ...
-
bioRxiv - Biochemistry 2021Quote: Nucleosome quality was analyzed by running the nucleosome on native PAGE on a 5% TBE gel in 0.5X TBE buffer (BioRad) that was run for 60 min at 150 volts ...
-
bioRxiv - Biochemistry 2021Quote: ... was added to a final concentration of 1X to the quenched reaction and samples were loaded onto a 5% TBE gel and run for 60 min at 150 volts in 0.5X TBE Buffer (BioRad). Gels were stained with ethidium bromide and imaged on a BioRad ChemiDoc ...
-
bioRxiv - Microbiology 2023Quote: ... pH 7.5] with 0.1% (v/v) Tween 20) containing 5% Blotting-Grade Blocker (Bio-Rad Laboratories, cat. #170-6404) for 30 mins ...