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Citations for Bio-Rad :
551 - 600 of 1458 citations for 2 CHLOROMETHYL 6 METHOXY 1H BENZO D IMIDAZOLE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 40S and 80S fractions were quantified using Quantity One 1-D Analysis Software v.4.1.2 (Bio-Rad Laboratories, Hercules, CA). Pno1 was quantified using ImageJ Software v1.52 (100 ...
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bioRxiv - Biochemistry 2021Quote: ... Melting temperature was determined from the region of maximal slope as visualized by the first derivative of fluorescence with respect to temperature (minimal-d(RFU)/dT) per default behavior of Bio-Rad CFX manager software (BioRad).
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (15 μg) were loaded on 4-15% 1 D polyacrylamide Mini-PROTEAN TGX Stain-Free gels (BioRad) and run in 1X Tris Glycine SDS buffer (BioRad ...
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bioRxiv - Immunology 2024Quote: ... and RANTES/CCL5 (R&D Biotechne) according to the manufacturer’s protocol and analyzed using the Bio-Plex 200 platform (Bio-rad). Analyte concentrations were calculated using a standard curve (5 PL regression ...
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bioRxiv - Biochemistry 2023Quote: ... 2mM DTT and 0.02% n-dodecyl-β-D-maltopyranoside [DDM]) buffer after transferring into Econo columns (Biorad, Cat. no. 7372512). Thrombin at concentration 1unit µL-1 was added to the resin slurry at 1:1 (dry resin:cleavage buffer ...
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bioRxiv - Biophysics 2024Quote: Claudins purified as described above were exchanged from DDM to 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside (LMNG) detergent via a PD-10 column (Bio-Rad). COP-1 was added in 1.4 moles excess to claudins then 1.4 moles excess Nb to COP-1 were mixed and incubated at 4°C for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane homogenates (50 μg) were separated on 6% SDS-PAGE gels and transferred onto presoaked PVDF membranes (Bio-Rad, Pleasanton, CA, USA). Blots were then blocked with 10% non-fat milk in PBST (100 nM phosphate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were run on 6% SDS-PAGE gels and blotted onto nitrocellulose using a semi-dry Trans-Blot (Bio-Rad, 1703940). Blots were incubated overnight at 4°C with a rabbit anti-myosin 18A polyclonal antibody that recognizes the C-terminal 18 residues of both myosin 18Aα and 18Aβ (Bruun et al. ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) in D2O using Micro Bio-Spin® Columns with Bio-Gel® P-6 (Bio-Rad Laboratories, Hercules, CA, USA). To avoid aggregation ...
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bioRxiv - Bioengineering 2021Quote: ... were desalted and buffer exchanged into 10 mM ammonium acetate using a spin column (Micro Bio-Spin™ P-6 Gel Columns, Bio-Rad). Octylamine was added to aliquots of NCB solution at a concentration of 0.1% (v/v) ...
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bioRxiv - Molecular Biology 2022Quote: ... The bombardment was performed (a distance of 6 cm using 1350 psi rupture disks) with a PDS-1000/He Biolistic Particle Delivery system (Bio-Rad, USA). The antibiotic concentration was determined after a sensitivity test ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was homogenized in 10 mM sodium acetate buffer (500 μL, pH 6.5) and filtered through Bio-spin 6 column (Bio-Rad, Hercules, CA) prewashed with 10 mM sodium acetate buffer (pH 6.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Purification of the desalted bacteriocin protein was performed using an Uno S-6 prepacked monolith cation exchange column (12×55 mm, Bio-Rad, USA) on a FPLC system (BioLogic DuoFlow System ...
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bioRxiv - Biochemistry 2020Quote: ... the desalted protein fraction was purified using the Uno S6 prepacked monolith cation exchange column (12 × 53 mm, 6 mL, Bio-Rad, USA). The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... SDS-PAGE and size exclusion chromatography (SEC) using a Superose® 6 Increase 10/300 GL column (Cytiva) and an NGC Quest 10 Plus System (Bio-Rad) showed that both wild-type and variant proteins were pure and monodisperse (Figure 2-figure supplement 1).
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bioRxiv - Microbiology 2024Quote: The tRNA modified product from MenT3 in vitro reactions underwent the following steps: it was initially purified using Bio-Spin® 6 Columns (Bio-Rad), followed by incubation with a putative exoribonuclease (10 µM ...
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bioRxiv - Genomics 2023Quote: ... The nuclei were isolated from cell homogenate by centrifugation and counted on an automated cell counter (TC20 BioRad, range 4-6 um). Transposition of 100k (weeks 1 ...
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bioRxiv - Biochemistry 2023Quote: ... followed by heat-inactivation at 75°C for 5 min and desalting using Micro Bio-Spin P-6 columns (Bio-Rad, 7326221). Dephosphorylated guide RNAs were then radiolabeled using T4 Polynucleotide Kinase (0.33 U/µL ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were rinsed in DBPS and moved to semi-solid media (6:4 ratio of 1% agarose (BIO-RAD, Hercules, CA, USA) in DPBS:DMEM with 10% FBS) ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (1 ml) (Healthcare Life Sciences) were loaded into disposable Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The qPCR was performed with a QuantStudio 6 Flex system equipped with a 384-well block and the SspAdvanced Unversal SYBR® Green Supermix (#1725272, BioRad, Switzerland) under the following conditions ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were separated in SDS/urea–PAGE (15% polyacrylamide, 6 M urea) (Laemmli, 1970) using mini-protean apparatus (Bio-Rad USA). These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA) ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 6% milk in PBS and subsequently stained with mouse anti-V5 (clone SV5-Pk1, BioRad, 1:10 000) and goat anti-mouse (1:100 000 ...
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bioRxiv - Microbiology 2024Quote: ... The samples were run on a low constant voltage at 4°C for 6 to 8 h in Tris Tricine SDS Running Buffer (Bio-Rad, USA). The peptides were detected by Instant Blue (Expedeon Inc.) ...
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bioRxiv - Microbiology 2024Quote: ... The samples were run on a low constant voltage at 4°C for 6 to 8 h in Tris Tricine SDS Running Buffer (Bio-Rad, USA). The peptides were detected by Instant Blue (Expedeon Inc.) ...
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bioRxiv - Immunology 2024Quote: ... were covalently coupled to carboxyl-functionalized fluorescent magnetic beads (1-3µg/1.25*10^6 beads) (Luminex Corp. MagPlex® Microspheres; Bio-Rad; Temse, Belgium) employing the BioPlex amine coupling kit (Ref. ...
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bioRxiv - Biophysics 2024Quote: ... The reaction volume was brought up to 50 μL by addition of MilliQ water and run through a Micro Bio-Spin™ 6 column (Biorad, 7326221) as per manufacturer instructions to purify radiolabelled oligonucleotide from free γ 32P ATP ...
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bioRxiv - Developmental Biology 2024Quote: ... and electroporated (square wave, 6 pulses, 30 V, 3 ms duration, 100 ms interval) using a gene Pulser XCell electroporator (Bio-Rad, 1652660). Zygotes were recovered and transferred to fresh KSOM drops and cultured overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Excess NEM or TCEP were removed by size exclusion chromatography with a 6 kDa cut-off spin column (Bio-Rad, UK, #7326222). After determining the protein content via a Bradford assay ...
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bioRxiv - Molecular Biology 2021Quote: ... Digitalized images of the destained SDS-PAGEs were analyzed by the Quantity One 1-D Analysis Software (Bio-Rad, Berkeley, US) to measure the band densities at each lane of all biological replicates ...
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bioRxiv - Cell Biology 2022Quote: ... as well as the level of cytokines in mouse serum, were assessed by using customized plates (Luminex assay, R&D) and analyzed on a Bio-Plex 200 instrument (Bio-Rad).
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bioRxiv - Plant Biology 2022Quote: ... Infiltrated leaves were infiltrated with 100 µM D-luciferin and LUC activity was quantified after 48 h using a low-light CCD imaging apparatus (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using oligo-d(T) primers and avian reverse transcriptase (iScript cDNA Synthesis Kit, Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... A horseradish peroxidase-conjugated secondary anti-mouse antibody (#HAF007, R&D) was applied and signals detected with a chemiluminescent HRP substrate using ChemiDocTM XR (Bio-Rad). Images were acquired using ImageLabTM software (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Cells were then transferred to a 2-mm cuvette (Bio-Rad) and electroporated at 300V for 2ms using an ECM830 Square Wave electroporator (BTX) ...
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bioRxiv - Genetics 2021Quote: ... or 2) the SsoAdvanced™ Universal Probes Supermix (1725284; Bio-Rad) with Taqman probes (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were assembled using 2× SsoFast EvaGreen Master Mix (Bio-Rad), 27F and 519R eubacterial 16S primers at 300 nM final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 500 μl of Bio-Beads SM-2 (Bio-Rad) overnight at 4°C on a nutator ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 mg of gel filtration protein standard (Bio-Rad 151-1901) was run after sample collection to estimate approximate molecular weight of protein peaks ...
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bioRxiv - Genomics 2020Quote: ... 50 mM EDTA and embedded in 2% CleanCut ™agarose (BioRad) in BioRad plug molds for a final agarose concentration of 0.75% ...
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bioRxiv - Cell Biology 2020Quote: ... n,n’-methylene-bis-acrylamide (BIORAD, 2% w/v stock solution), N-6-((acryloyl)amino)hexanoic acid crosslinker (N6 ...
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bioRxiv - Biochemistry 2021Quote: An ATP purification column was prepared using P-2 gel (Biorad). In 10 mL of P-2 wash buffer (10 mM Tris·HCl pH 8 ...
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bioRxiv - Bioengineering 2020Quote: ... Custom primers were used for gene amplification (Table 2, Bio-Rad)37,38.
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bioRxiv - Bioengineering 2020Quote: ... The membranes were blocked with 2% Blotting-Grade Blocker (Biorad,1706404) in TBS-T (25 mM Tris–HCl ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were diluted with 2× Laemmli sample buffer (Bio-Rad) and boiled at 95°C for 10 min and then underwent western blotting ...
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bioRxiv - Plant Biology 2020Quote: ... 2% low melting agarose solution (Certified™ Low Melt Agarose, BioRad) was prepared using 1X TBE buffer (Tris base 1M ...
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bioRxiv - Neuroscience 2020Quote: ... 40% Acrylamide and 2% bis-acrylamide (Bio-Rad Laboratories, Richmond, CA) were mixed at ratios of 5% ...
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bioRxiv - Biophysics 2021Quote: ... Detergent was removed by addition of Bio-Beads SM-2 (Biorad) and incubation at 4 °C overnight ...
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bioRxiv - Biophysics 2020Quote: ... The detergent was removed with Bio-Beads SM-2 (Bio-Rad) for 4 hours ...