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Citations for Bio Basic :
1 - 24 of 24 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... in 1x annealing buffer (5 mM Tris, 1 mM EDTA, 5 mM MgCl, Millipore, cat# 648311-1KG, Bio Basic, cat# SD8135, Bio Basic, cat# MRB0328) and placed into the thermocycler ...
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bioRxiv - Cell Biology 2020Quote: ... ten 15 cm dishes of U2OS cells stably expressing BirA-HAUS6 or parental U2OS cells (negative control) were incubated in culture medium containing 50 μM Biotin (Bio Basic) overnight ...
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bioRxiv - Microbiology 2022Quote: ... A puc19 plasmid containing a RIX/NSP3-2A-P-His insert under the control of a T7 transcription promoter (puc19/T7/ RIX/NSP3-2A-P-His) was purchased from Bio Basic Canada Inc ...
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bioRxiv - Microbiology 2023Quote: ... The second transfection was done with pJET-UL39R-GFP-p53-Ul39L plasmid supplemented with 10 ug/mL of puromycin (Bio Basic, Canada). After 48h ...
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bioRxiv - Bioengineering 2023Quote: ... were mixed with ssDNA output oligonucleotides of interest at 2x excess (Table S1) in 1x annealing buffer (5 mM Tris, 1 mM EDTA, 5 mM MgCl, Millipore, cat# 648311-1KG, Bio Basic, cat# SD8135, Bio Basic, cat# MRB0328) and placed into the thermocycler ...
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bioRxiv - Cell Biology 2019Quote: ... Knock-out cell lines were used to prepare over-expression cell lines by transfection of a pcDNA3.1(-) plasmid expressing the complete human CasD1 cDNA open reading frame synthesized by Bio Basic (Markham, Ontario, Canada). Transfected cells were selected with G418 and single cell clones screened by staining with PToV-P4 HE-Fc to identify 9-O-Ac positive cell lines ...
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bioRxiv - Genetics 2019Quote: ... containing 5% milk (Bio Basic, cat #NB0669). Membranes were then stained with PTEN antibody (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: Miniprep kit (Bio Basic, cat. no. BS614)
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bioRxiv - Molecular Biology 2022Quote: ... Tryptone (TT) (Bio basic, Cat no. TG217 (G211)) ...
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bioRxiv - Molecular Biology 2022Quote: ... or 1 mM MTX (Bio Basic Inc, Cat# MB0612) was added where relevant and the sample preheated at 37°C for 2 min ...
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bioRxiv - Systems Biology 2020Quote: ... and 10 μg of RNase A (Bio Basic, Markham, ON, Canada, Cat# RB0473) and incubated on ice for 5 minutes with occasional agitation of the plate ...
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bioRxiv - Plant Biology 2021Quote: ... and RNA was extracted using an RNA isolation kit (Bio Basic; Cat#BS82314). ProtoScript II reverse transcriptase (NEB ...
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TIR signaling promotes the interactions between EDS1/PAD4 and ADR1-L1 and oligomerization of ADR1-L1bioRxiv - Plant Biology 2021Quote: ... and RNA was extracted using an RNA isolation kit (Bio Basic; Cat#BS82314). ProtoScript II reverse transcriptase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... After washing with Tris-buffered saline (cat. no. A0027, BIO BASIC, Markham ON, Canada) membranes were treated with an HRP-linked secondary antibody (Anti-rabbit IgG cat ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using 5X All-in-One RT Master Mix (Bio Basic cat# HRT025-10) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed using Green-2-Go qPCR Master Mix (Bio Basic cat# QPCR004-S) with 25 ng cDNA per reaction and primers targeting mouse Sumo1 (NM_009460.2 ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using 5X All-in-One RT Master Mix Kit (Bio Basic cat# HRT025-10). Real-time quantitative PCR was performed using Green-2-Go qPCR Master Mix (Bio Basic cat# QPCR004-S ...
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bioRxiv - Genomics 2023Quote: ... total RNAs were extracted with the EZ-10 DNAaway RNA Mini-Preps kit (Bio Basic, cat# BS88136) as recommended ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time quantitative PCR was performed using Green-2-Go qPCR Master Mix (Bio Basic cat# QPCR004-S) with 25 ng cDNA per reaction and primers targeting mouse Gapdh (Forward ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with antibodies in a solution of Tris-buffered saline (cat. no. A0027, BIO BASIC, Markham ON, Canada) at 4°C for overnight ...
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bioRxiv - Immunology 2023Quote: ... cells were harvested either with RIPA buffer for Western blots or with total RNA Miniprep Super Kit for total RNA purification (Cat: B610583, Bio Basic) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: 6-well cell culture plates (Cell Star, Cat. -No. 657 160) were prepared by melting agarose (purchased from Bio Basic Canada Inc.; AB0015) pouring 3 mL into each well ...
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bioRxiv - Cell Biology 2021Quote: ... bacteria cells were spin down and suspended in buffer H (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% Glycerol) containing 1 mg/ml lysozyme (Bio Basic, Cat. No. LDB0308-5) and 0.1 % TritonX-100 ...
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bioRxiv - Molecular Biology 2022Quote: All the samples used for cryo-EM were prepared using freshly purified and concentrated nanodisc-reconstituted hMRP4 and freshly prepared stocks of ATP (Bio Basic Inc, Cat#AB0020), ATPγS (Roche ...