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Citations for Bio Basic :
1 - 17 of 17 citations for SARS Coronavirus Membrane Protein Matrix E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: The gene encoding 419-residue SARS-CoV-2 N protein was purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was cloned into an expression vector pET-28a with a TEV protease cleavage site between N protein and N-terminal 6xHis-SUMO tag used to enhance the solubility ...
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bioRxiv - Biophysics 2022Quote: The gene encoding 419-residue SARS-CoV-2 N protein was purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was cloned into an expression vector pET-28a with a TEV protease cleavage site between N protein and N-terminal 6xHis-SUMO tag used to enhance the solubility (10) ...
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bioRxiv - Biochemistry 2022Quote: ... coli BL21 (DE3) by BIO BASIC Inc (Markham ON ...
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bioRxiv - Microbiology 2023Quote: ... coli expression and synthesized commercially by Bio Basic Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... coli expression and was cloned into pET15b vector (Bio Basic) and transformed into the E ...
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bioRxiv - Biochemistry 2021Quote: ... coli using an EZ-10 total RNA purification kit (Bio Basic) and the integrity/purity confirmed using formaldehyde agarose gel electrophoresis and A260/A280 ratio (Biodrop) ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S-expressing plasmids were codon-optimized and generated by gene synthesis (Bio Basic Asia Pacific) according to GenBank accession QHD43416.1 ...
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bioRxiv - Biochemistry 2022Quote: The gene encoding SARS-CoV-2 2-170 NSP3 Mac1 was cloned into a pET-11a plasmid (Bio Basic) and transformed into E ...
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bioRxiv - Microbiology 2020Quote: ... the entire genome sequence of SARS-CoV-2 USA/WA1/2020 (GenBank accession no. MN985325) was chemically synthesized (Bio Basic) in five fragments and cloned into pUC57 plasmids containing unique restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... a set of overlapping cDNA fragments representing the entire genome of SARS-CoV-2 Wuhan isolate (GenBank: MN908947.3) were chemically synthesized and cloned into pUC57-Kan (Bio Basic Canada Inc). Where appropriate the relevant synthetic cDNA fragment carried the mutation D614G or Y453F + D614G in the viral S gene ...
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bioRxiv - Cancer Biology 2020Quote: Cell lysate protein concentration was determined using a Micro BCA Protein Assay kit (Bio Basic). Unless specified ...
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bioRxiv - Biochemistry 2022Quote: ... Escherichia coli BL21 Rosetta (DE3) cells harboring these plasmids were induced with 0.2 mM isopropyl β-D-1-thiogalactopyranoside (Bio Basic) for 16 hours at 16 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... heat shock transformation was performed using Escherichia coli (DH5α) Plasmids were purified using EZ-10 Spin Column Plasmid DNA Miniprep Kit by (Bio Basic Inc). Correct gRNA insertion was confirmed by DNA sequencing.
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bioRxiv - Bioengineering 2019Quote: ... Loading volumes of 10-15 μg of protein per well were calculated using a MicroBCA Protein Assay Kit (Bio Basic, SK3061) according to the manufacturer’s specification ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked using 5% milk in Tris-Buffered Saline (Bio Basic, Markham, Ontario, Canada) with Tween-20 for 45 minutes at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... Protein quantification was done by Bradford method (Bio Basic Inc. USA) using Bovine Serum Albumin (BSA ...
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bioRxiv - Systems Biology 2020Quote: ... Protein production was then induced by the addition of 1 mM IPTG (Bio Basic) and incubation at 16°C overnight with agitation ...