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Citations for Bio Basic :
1 - 39 of 39 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Constructs were amplified from insect cell codon-optimized DNA fragments (Bio Basic Inc., Canada) for protein production in High Five insect cells ...
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bioRxiv - Cancer Biology 2020Quote: Cell lysate protein concentration was determined using a Micro BCA Protein Assay kit (Bio Basic). Unless specified ...
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bioRxiv - Systems Biology 2020Quote: ... Protein production was then induced by the addition of 1 mM IPTG (Bio Basic) and incubation at 16°C overnight with agitation ...
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bioRxiv - Bioengineering 2019Quote: ... Loading volumes of 10-15 μg of protein per well were calculated using a MicroBCA Protein Assay Kit (Bio Basic, SK3061) according to the manufacturer’s specification ...
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bioRxiv - Biophysics 2019Quote: ... Protein quantification was done by Bradford method (Bio Basic Inc. USA) using Bovine Serum Albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... Escherichia coli BL21 Rosetta (DE3) cells harboring these plasmids were induced with 0.2 mM isopropyl β-D-1-thiogalactopyranoside (Bio Basic) for 16 hours at 16 °C ...
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bioRxiv - Zoology 2020Quote: Total RNA was isolated from 1×106 Xela DS2 or Xela VS2 cells using the EZ-10 Spin Column Total RNA Minipreps Super Kit (Bio Basic Canada Inc.) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: The gene encoding 419-residue SARS-CoV-2 N protein was purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was cloned into an expression vector pET-28a with a TEV protease cleavage site between N protein and N-terminal 6xHis-SUMO tag used to enhance the solubility ...
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bioRxiv - Biophysics 2022Quote: The gene encoding 419-residue SARS-CoV-2 N protein was purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was cloned into an expression vector pET-28a with a TEV protease cleavage site between N protein and N-terminal 6xHis-SUMO tag used to enhance the solubility (10) ...
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bioRxiv - Microbiology 2021Quote: ... source 1 agar (BIO BASIC, FB0010), source 2 Agar (Sangon Biotech ...
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bioRxiv - Immunology 2023Quote: ... The tumor cell suspension was filtered (100µm) and red blood cells were lysed using 0.83% NH4Cl (Bio Basic; CAS #12125-02-9) for 5 min at RT ...
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bioRxiv - Biophysics 2021Quote: 6-well cell culture plates (Cell Star, Cat. -No. 657 160) were prepared by melting agarose (purchased from Bio Basic Canada Inc.; AB0015) pouring 3 mL into each well ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 1/10 volume of 1.5M Tris-HCl pH 8.8 (Tris Base Fisher BP152-5, Hydrochloric Acid Fisher A144-212) and 1/10 volume 1 M DTT (Bio Basic DB0058). Samples were boiled for 5 minutes before an additional centrifugation at 4 °C at 16,000 x g for 4 minutes.
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bioRxiv - Microbiology 2021Quote: ... 50 µg mL−1 of kanamycin (Kan; Bio Basic).
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bioRxiv - Molecular Biology 2022Quote: ... or 1 mM MTX (Bio Basic Inc, Cat# MB0612) was added where relevant and the sample preheated at 37°C for 2 min ...
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bioRxiv - Developmental Biology 2023Quote: ... covered with a 1% agar pad (Agar A, Bio Basic, FB0010 ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast strains were grown in YPD (1% yeast extract (Bio Basic), 2% peptone (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and 140 mM NaCl (SB0476-1; Bio Basic, Markham, Ontario, Canada) in ddH2O] for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... 1% sodium dodecyl sulfate (SDS) and 4X Denhardt’s solution (Bio Basic D0062)] ...
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bioRxiv - Microbiology 2023Quote: ... traNAhD4-1 and traNAQU1 were chemically synthesized and obtained from Bio Basic. sfx94 ...
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bioRxiv - Cell Biology 2024Quote: ... and then adding 1mM IPTG (Bio Basic item number 367-93-1) for 4 hours at 37°C with shaking ...
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bioRxiv - Cell Biology 2022Quote: ... 24h post infection cells were selected with puromycin (PJ593; Bio Basic, Markham, ON, Canada) 0,75ug for MRC-5 and 2ug for HeLa for 48-72h.
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bioRxiv - Genomics 2019Quote: DNA from each cell line was extracted using EZ-10 Spin Columns (Bio Basic #BS427) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Xela VS2 and EPC cells using the EZ-10 Spin Column Total RNA Minipreps Super Kit (Bio Basic) according to the manufacturer’s specifications with modifications to include an on-column DNase I digestion as described previously by our group (Bui-Marinos et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... Citric acid monohydrate (CAS# 5949-29-1) and sodium citrate (CAS# 6132-04-3) from Bio basic Canada Inc ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were lysed and RNA was extracted using EZ-10 DNAaway RNA Mini-Prep Kit (Bio Basic inc). cDNA was synthesized using Superscript IV (Thermo Fischer ...
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bioRxiv - Molecular Biology 2023Quote: RNA was extracted from tissue culture cells using either the EZ-10 spin column kit (Bio Basic, Markham, Canada) or the MagMAX mirVana Total RNA Isolation Kit (Thermo Fisher Scientific A27828 ...
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bioRxiv - Microbiology 2021Quote: Total RNA was isolated from lysed cells using an EZ-10 spin column total RNA minipreps super kit (Bio Basic) following manufacture instructions ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was isolated from cells after the indicated treatments using the EZ-10 DNAaway RNA Miniprep kit (Bio Basic). Reverse transcription to generate cDNAs was performed using the qScript cDNA SuperMix (Quantabio) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with antibodies in a solution of Tris-buffered saline (cat. no. A0027, BIO BASIC, Markham ON, Canada) at 4°C for overnight ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was isolated from combined adherent and suspension cells using the EZ-10 Spin Column Total RNA Minipreps Super Kit (Bio Basic) with the following modification ...
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bioRxiv - Cell Biology 2020Quote: ... ten 15 cm dishes of U2OS cells stably expressing BirA-HAUS6 or parental U2OS cells (negative control) were incubated in culture medium containing 50 μM Biotin (Bio Basic) overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Strains expressing the RPB1 CTD WT or CTD mutant plasmids were grown in YNB medium lacking histidine to an OD600 of 0.5 and treated with 1 μg/mL of rapamycin (Bio Basic) for 90 min to anchor-away the endogenous Rpb1 protein ...
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bioRxiv - Microbiology 2021Quote: ... Samples were incubated for 60 min at 37°C with 10 pg µl-1 of RNase A (Bio Basic, Toronto, Canada). RNase activity was inactivated by adding 100 unites of Protector RNase Inhibitor (Roche ...
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bioRxiv - Biochemistry 2023Quote: A plasmid for bacterial expression of human ARL15 (1-204) was obtained by cloning codon-optimized synthetic DNA into the NdeI and BamHI cloning sites of pET15b (Bio Basic). A truncated construct (residues 32-197 ...
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bioRxiv - Plant Biology 2019Quote: ... The target loci were amplified by PCR using specific primers (Supplementary Table 1) and resulting DNA fragments were purified with PCR purification kit (Bio Basic Inc, Canada). The wsl5 and zebra3 PCR product was digested with SacI and SalI ...
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bioRxiv - Cell Biology 2021Quote: ... bacteria cells were spin down and suspended in buffer H (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% Glycerol) containing 1 mg/ml lysozyme (Bio Basic, Cat. No. LDB0308-5) and 0.1 % TritonX-100 ...
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bioRxiv - Bioengineering 2023Quote: ... in 1x annealing buffer (5 mM Tris, 1 mM EDTA, 5 mM MgCl, Millipore, cat# 648311-1KG, Bio Basic, cat# SD8135, Bio Basic, cat# MRB0328) and placed into the thermocycler ...
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bioRxiv - Bioengineering 2023Quote: ... were mixed with ssDNA output oligonucleotides of interest at 2x excess (Table S1) in 1x annealing buffer (5 mM Tris, 1 mM EDTA, 5 mM MgCl, Millipore, cat# 648311-1KG, Bio Basic, cat# SD8135, Bio Basic, cat# MRB0328) and placed into the thermocycler ...