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Citations for Bio Basic :
1 - 19 of 19 citations for CEACAM 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... A puc19 plasmid containing a RIX/NSP3-2A-P-His insert under the control of a T7 transcription promoter (puc19/T7/ RIX/NSP3-2A-P-His) was purchased from Bio Basic Canada Inc ...
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bioRxiv - Genetics 2019Quote: ... containing 5% milk (Bio Basic, cat #NB0669). Membranes were then stained with PTEN antibody (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Os12t0613700-01) genes were codon optimized for humans and synthesized from Bio Basic Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... Forward primer LCHV-MEP93-qF: 5’-GTACTTCATCGCCTACGGAGC-3’ and reverse primer LCHV-MEP93-qR: 5’-TACGTGTGCTTGAGGAGGTC-3’ were synthesized from Bio Basic, Canada ...
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bioRxiv - Bioengineering 2023Quote: ... in 1x annealing buffer (5 mM Tris, 1 mM EDTA, 5 mM MgCl, Millipore, cat# 648311-1KG, Bio Basic, cat# SD8135, Bio Basic, cat# MRB0328) and placed into the thermocycler ...
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bioRxiv - Bioengineering 2023Quote: ... were mixed with ssDNA output oligonucleotides of interest at 2x excess (Table S1) in 1x annealing buffer (5 mM Tris, 1 mM EDTA, 5 mM MgCl, Millipore, cat# 648311-1KG, Bio Basic, cat# SD8135, Bio Basic, cat# MRB0328) and placed into the thermocycler ...
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bioRxiv - Biophysics 2021Quote: Genes encoding full-length human and zebrafish TRPM5 (UniProtKB accession numbers Q9NZQ8, and S5UH5, respectively) were synthesized by Bio Basic and were sub-cloned into a pEG BacMam vector with an His8 tag ...
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bioRxiv - Microbiology 2022Quote: ... NaHCO3 or FBS but supplemented with 5 ng/mL IFN-γ (Bio Basic, USA), 400 ng/mL LPS (EMD Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked using 5% milk in Tris-Buffered Saline (Bio Basic, Markham, Ontario, Canada) with Tween-20 for 45 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Knock-out cell lines were used to prepare over-expression cell lines by transfection of a pcDNA3.1(-) plasmid expressing the complete human CasD1 cDNA open reading frame synthesized by Bio Basic (Markham, Ontario, Canada). Transfected cells were selected with G418 and single cell clones screened by staining with PToV-P4 HE-Fc to identify 9-O-Ac positive cell lines ...
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bioRxiv - Immunology 2021Quote: ... and RNA was reverse-transcribed into cDNA using the 5 × All-In-One RT Master Mix (Bio Basic) according to the manufacturer’s specifications (25 °C for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... Microglia were polarized to M2 with 50 ng/ml interleukin-4 (IL-4, Bio Basic, RC212-15-5) for 48 hours.
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bioRxiv - Bioengineering 2023Quote: Macrophages were polarized to M2 with 50 ng/ml interleukin-4 (IL-4, Bio Basic, RC212-15-5) for 48 hours (Ext Fig 1B) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 1/10 volume of 1.5M Tris-HCl pH 8.8 (Tris Base Fisher BP152-5, Hydrochloric Acid Fisher A144-212) and 1/10 volume 1 M DTT (Bio Basic DB0058). Samples were boiled for 5 minutes before an additional centrifugation at 4 °C at 16,000 x g for 4 minutes.
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bioRxiv - Biochemistry 2020Quote: The plasmid containing the monomer of a 145 bp of telomeric DNA (5′-ATC-(TTAGGG)23TGAT-3′) flanked by EcoRV and AvaI sites was obtained from Bio Basic Asia Pacific Pte Ltd ...
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bioRxiv - Cell Biology 2023Quote: ... alkaline phosphatase conjugated antibodies were detected colourometrically using BCIP/NBT (nitro-blue tetrazolium and 5- bromo-4-chloro-3’-indolyphosphate) Substrate Solution (#BE116.100ML; Bio Basic Canada Inc). For analysis of bands ...
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bioRxiv - Biophysics 2020Quote: ... the expression vector of the tethered RRM1-RRM2 (5-184) of hnRNPA1 were purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was subsequently dissected into the isolated RRM1 (5-95 ...
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bioRxiv - Cell Biology 2021Quote: ... bacteria cells were spin down and suspended in buffer H (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% Glycerol) containing 1 mg/ml lysozyme (Bio Basic, Cat. No. LDB0308-5) and 0.1 % TritonX-100 ...
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bioRxiv - Immunology 2020Quote: ... Lm-OVA stocks frozen at −80 C were grown overnight at 37°C in BHI broth supplemented with 5 ug/ml Erythromycin (Bio Basic, Amherst, New York). Then ...