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Citations for Bio Basic :
1 - 25 of 25 citations for 6 Chlorospiro chroman 2 4' piperidin 4 one hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Genomic DNA was isolated using the Bio Basic All-4-One Genomic DNA MiniPrep Kit flowing the manufacturer’s protocol including the RNase treatment (Bio Basic Inc., Markham ON, Canada). The region around the CRISPR PREX1 target site was PCR-amplified using the primer pair DPREX3F (5’-GCACAGAGGGAAAGTCTCGG-3’ ...
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bioRxiv - Bioengineering 2023Quote: ... Microglia were polarized to M2 with 50 ng/ml interleukin-4 (IL-4, Bio Basic, RC212-15-5) for 48 hours.
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bioRxiv - Bioengineering 2023Quote: Macrophages were polarized to M2 with 50 ng/ml interleukin-4 (IL-4, Bio Basic, RC212-15-5) for 48 hours (Ext Fig 1B) ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... They were fixed in 4% paraformaldehyde for 24 h (Bio Basic, Markham, Ontario, Canada), decalcified in 10% EDTA-disodium salt for 6–8 weeks (Kelong ...
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bioRxiv - Genetics 2020Quote: ... and were then fixed using perilymphatic perfusion with 4% paraformaldehyde (PFA) (Bio Basic Inc.,) prepared in phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... alkaline phosphatase conjugated antibodies were detected colourometrically using BCIP/NBT (nitro-blue tetrazolium and 5- bromo-4-chloro-3’-indolyphosphate) Substrate Solution (#BE116.100ML; Bio Basic Canada Inc). For analysis of bands ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using 5X All-in-One RT Master Mix (Bio Basic cat# HRT025-10) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using 5X All-in-One RT Master Mix Kit (Bio Basic cat# HRT025-10). Real-time quantitative PCR was performed using Green-2-Go qPCR Master Mix (Bio Basic cat# QPCR004-S ...
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bioRxiv - Immunology 2021Quote: ... and RNA was reverse-transcribed into cDNA using the 5 × All-In-One RT Master Mix (Bio Basic) according to the manufacturer’s specifications (25 °C for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was isolated with All-in-One DNA/RNA Mini-Preps Kit according to the manufacturer’s protocol (Bio Basic) and reverse transcribed using either a QuantiTect Revese Transcription Kit (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: The total RNA of plant and microbe samples were extracted using an All-In-One DNA/RNA Mini-preps Kit (Bio Basic) following operation manual ...
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bioRxiv - Biochemistry 2022Quote: The gene encoding SARS-CoV-2 2-170 NSP3 Mac1 was cloned into a pET-11a plasmid (Bio Basic) and transformed into E ...
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bioRxiv - Immunology 2024Quote: ... 1.5 mM MgCl2 and 2 mM MgSO4 (Bio Basic) in a total volume of 20 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... selection was performed using 2 µg/ml of puromycin (Bio Basic) to collect cells expressing the desired shRNAs ...
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bioRxiv - Biophysics 2021Quote: 6-well cell culture plates (Cell Star, Cat. -No. 657 160) were prepared by melting agarose (purchased from Bio Basic Canada Inc.; AB0015) pouring 3 mL into each well ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed using Green-2-Go qPCR Master Mix (Bio Basic cat# QPCR004-S) with 25 ng cDNA per reaction and primers targeting mouse Sumo1 (NM_009460.2 ...
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bioRxiv - Microbiology 2020Quote: GM (2 mg/mL) solution was prepared using gentamicin sulfate of USP grade produced by BIO BASIC INC (Toronto ...
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bioRxiv - Evolutionary Biology 2023Quote: The wild-type (wt) VIM-2 gene including its signal peptide sequence was synthesized (Bio Basic Inc.) and subcloned into a low-copy number plasmid ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time quantitative PCR was performed using Green-2-Go qPCR Master Mix (Bio Basic cat# QPCR004-S) with 25 ng cDNA per reaction and primers targeting mouse Gapdh (Forward ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S-expressing plasmids were codon-optimized and generated by gene synthesis (Bio Basic Asia Pacific) according to GenBank accession QHD43416.1 ...
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bioRxiv - Microbiology 2020Quote: ... the entire genome sequence of SARS-CoV-2 USA/WA1/2020 (GenBank accession no. MN985325) was chemically synthesized (Bio Basic) in five fragments and cloned into pUC57 plasmids containing unique restriction sites ...
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bioRxiv - Biophysics 2024Quote: ... The mutant ΔPAS EAG1 channel in pGH19 with the deletion of the PAS domain residues 2-130 was generated by Bio Basic Inc ...
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bioRxiv - Microbiology 2021Quote: ... a set of overlapping cDNA fragments representing the entire genome of SARS-CoV-2 Wuhan isolate (GenBank: MN908947.3) were chemically synthesized and cloned into pUC57-Kan (Bio Basic Canada Inc). Where appropriate the relevant synthetic cDNA fragment carried the mutation D614G or Y453F + D614G in the viral S gene ...
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bioRxiv - Biophysics 2021Quote: The gene encoding 419-residue SARS-CoV-2 N protein was purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was cloned into an expression vector pET-28a with a TEV protease cleavage site between N protein and N-terminal 6xHis-SUMO tag used to enhance the solubility ...
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bioRxiv - Biophysics 2022Quote: The gene encoding 419-residue SARS-CoV-2 N protein was purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was cloned into an expression vector pET-28a with a TEV protease cleavage site between N protein and N-terminal 6xHis-SUMO tag used to enhance the solubility (10) ...