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Citations for Epoch Life Science :
1 - 26 of 26 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: The full-length human RANBP2 sequence was codon optimized for expression in Sf9 insect cells (Epoch Life Sciences). Residues 2148-2240 fused to a N-terminal GCN4 dimerization tag were subcloned into a pACEBac1 vector with a N-terminal 6xHis-ZZ tag followed by a TEV cleavage site ...
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bioRxiv - Molecular Biology 2023Quote: DNA was extracted from each tagged colony using the GenCatch genomic DNA extraction kit (Epoch Life Science, Sugar Land, TX) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant transfer vector p72mCheryΔI177L was obtained by DNA synthesis (Epoch Life Sciences Missouri City, TX, USA).
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bioRxiv - Biochemistry 2023Quote: ... and σNS-ΔN17 were subcloned into the bacterial expression vector pET28 with an N-terminal His tag and a TEV protease cleavage site (Epoch Life Science). Escherichia coli DE3 cells (Novagen ...
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bioRxiv - Biophysics 2024Quote: cDNAs for human Cx26 and Cx30 were synthesized and subcloned by Epoch Life Science into pGEM-HA (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli codon-optimised genes encoding each full-length protein and isoform 2 of CPSF30 (Uniprot O95639-2) in pACEBac vectors were synthesized by Epoch Life Science. All cloning was validated by sequencing (Source Bioscience).
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bioRxiv - Immunology 2022Quote: ... and 143 mM 2-mercaptoethanol and purified on silica columns (Epoch Life Science). Using 500 ng of RNA ...
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bioRxiv - Bioengineering 2020Quote: ... The 300 bp resulting DNA was then gel extracted from a 2% agarose gel and purified via silicon spin column (Epoch Life Sciences) eluting with 30 μL dH2O per manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Mammalian expression vectors were generated by inserting c-myc tag and mRuby3 red fluorescent protein upstream of full length NS1-2 and then subcloning this into the pTagRFP-N vector in place of TagRFP (Epoch Life Sciences, Missouri City, TX). This construct will be referred to as RFP-NS1-2 ...
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bioRxiv - Microbiology 2022Quote: ... A 450 µl aliquot of the homogenate was then transferred to a sterile 2 ml EconoSpin mini spin column (catalog no. 1910-250, Epoch Life Science, Inc., Sugar Land, TX, USA) containing 450 µl of 70% (v/v ...
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bioRxiv - Immunology 2022Quote: DH5α Competent Cells were mixed with pCMV-IRES2-EGFP plasmids (Epoch Life sciences) for expressing a gene together with EGFP ...
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bioRxiv - Bioengineering 2020Quote: ... 10 ng of the oligopool resuspended at 1 ng/μL in EB (Epoch Life Science), diluted to 50 μL total volume with dH2O ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA was extracted from 1 mL of suspended culture via miniprep (Epoch Life Science). The transformation yielded approximately 21 million colonies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1999) and 1 GRE (5’AGAACAGT3’ > 5’TAGCATCT3’) were generated by site-directed mutagenesis (Epoch Life Sciences). Transactivation Assays ...
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bioRxiv - Cell Biology 2022Quote: ... The LRRK1 sequence was codon optimized for expression in Spodoptera frugiperda (Sf9) cells and synthesized by Epoch Life Science. The DNA coding for wild-type LRRK1 residues 631-2015 (LRRK1RCKW ...
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bioRxiv - Biochemistry 2022Quote: The following Dynactin coding genes were codon-optimized for protein expression in insect cells and synthesized (Epoch Life Science): DCTN1 (Protein name p150 ...
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bioRxiv - Biochemistry 2022Quote: ... the DNA coding for LRRK1 was codon optimized for Spodoptera frugipera (Sf9) cells and synthesized by Epoch Life Science. The DNA was cloned via Gibson assembly into the pKL baculoviral expression vector ...
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bioRxiv - Synthetic Biology 2022Quote: ... followed by 1% agarose gel electrophoresis and gel purification using a gel extraction kit (Epoch Life Science, 2260050). Recovered PCR products were digested with SpeI-HF and MluI-HF ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells were transfected with pcDNA3_HALO and pcDNA3_HALO-EBP plasmids that we generated from a Pax7-HALO plasmid (Epoch Life Science) using PEI as aforementioned ...
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bioRxiv - Microbiology 2024Quote: The full-length mouse BICD2 sequence (UniProt Q921C5) was codon optimized for expression in Sf9 insect cells (Epoch Life Sciences) and cloned into a pACEBac1 vector encoding a N-terminal Strep-tag followed a PreScission (Psc ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA was purified from cells using a Qiagen 96 Plus Miniprep Kit and Econospin Miniprep Filter plates (Epoch Life Science). Purified plasmids were prepared for sequencing using a Tn5 transposase and tagmentation approach and sequenced using an Illumina MiSeq ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA was purified from cells using a Qiagen 96 Plus Miniprep Kit and Econospin Miniprep Filter plates (Epoch Life Science). Purified plasmids were prepared for sequencing using a Tn5 transposase and tagmentation approach and sequenced using an Illumina MiSeq ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified using EconoSpin TM All-in-1 Mini Spin Columns (Epoch Life Science, 1920-250) and eluted with RNase free water ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA was purified from cells using a Qiagen 96 Plus Miniprep Kit and Econospin Miniprep Filter plates (Epoch Life Science, 2020-001). Purified plasmids were prepared for sequencing using a Tn5 transposase and tagmentation approach and sequenced using an Illumina MiSeq ...
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bioRxiv - Synthetic Biology 2021Quote: ... at 37°C for 1 hour and agarose gel-purified with GenCatch Advanced Gel Extraction Kit (Epoch Life Science, Inc., No. 2260050). eGFP fluorescence was measured at λex 488 nm and λem 509 nm with plate reader PMT setting “medium” and 6 reads per well ...
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bioRxiv - Neuroscience 2019Quote: ... and EGFP genes into the pAAV-CAG vector to obtain the final constructs described in Supplementary Table 1 (gene synthesis and subcloning were performed by Epoch Life Science Inc.). For in vivo expression in the mouse brain via in utero electroporation (IUE) ...