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1 - 10 of 10 citations for Recombinant Human Carboxylesterase 2 His tagged since 2019

• Transcriptomic signatures across a critical sedimentation threshold in a major reef-building coral

  Colin Lock, Melissa M. Gabriel, Bastian Bentlage, bioRxiv - Molecular Biology 2023

  Quote: DNA was extracted from each tagged colony using the GenCatch genomic DNA extraction kit (Epoch Life Science, Sugar Land, TX) following the manufacturer’s protocol for tissue samples ...
• Development of a highly effective African swine fever virus vaccine by deletion of the I177L gene results in sterile immunity against the current epidemic Eurasia strain

  Manuel V. Borca, et al., bioRxiv - Microbiology 2019

  Quote: ... Recombinant transfer vector p72mCheryΔI177L was obtained by DNA synthesis (Epoch Life Sciences Missouri City, TX, USA).
• Structure of Orthoreovirus RNA Chaperone σNS, a Component of Viral Replication Factories

  Boyang Zhao, et al., bioRxiv - Biochemistry 2023

  Quote: ... and σNS-ΔN17 were subcloned into the bacterial expression vector pET28 with an N-terminal His tag and a TEV protease cleavage site (Epoch Life Science). Escherichia coli DE3 cells (Novagen ...
• Large-pore connexin hemichannels function as molecule transporters independently of ion conduction

  Pablo S. Gaete, et al., bioRxiv - Biophysics 2024

  Quote: cDNAs for human Cx26 and Cx30 were synthesized and subcloned by Epoch Life Science into pGEM-HA (Promega) ...
• RBBP6 activates the pre-mRNA 3’-end processing machinery in humans

  Vytaute Boreikaite, et al., bioRxiv - Molecular Biology 2021

  Quote: ... coli codon-optimised genes encoding each full-length protein and isoform 2 of CPSF30 (Uniprot O95639-2) in pACEBac vectors were synthesized by Epoch Life Science. All cloning was validated by sequencing (Source Bioscience).
• The intracellular bacterium Orientia tsutsugamushi hijacks the adaptor protein BICD2 for dynein-based motility

  Giulia Manigrasso, et al., bioRxiv - Microbiology 2024

  Quote: The full-length human RANBP2 sequence was codon optimized for expression in Sf9 insect cells (Epoch Life Sciences). Residues 2148-2240 fused to a N-terminal GCN4 dimerization tag were subcloned into a pACEBac1 vector with a N-terminal 6xHis-ZZ tag followed by a TEV cleavage site ...
• DNA-PKcs is required for cGAS/STING-dependent viral DNA sensing in human cells

  Dayana Hristova, et al., bioRxiv - Immunology 2022

  Quote: ... and 143 mM 2-mercaptoethanol and purified on silica columns (Epoch Life Science). Using 500 ng of RNA ...
• High-Throughput Developability Assays Enable Library-Scale Identification of Producible Protein Scaffold Variants

  Alexander W. Golinski, et al., bioRxiv - Bioengineering 2020

  Quote: ... The 300 bp resulting DNA was then gel extracted from a 2% agarose gel and purified via silicon spin column (Epoch Life Sciences) eluting with 30 μL dH2O per manufacturer’s instructions.
• Recovirus NS1-2 has viroporin activity that induces aberrant cellular calcium signaling to facilitate virus replication

  Alicia C. Strtak, et al., bioRxiv - Microbiology 2019

  Quote: ... Mammalian expression vectors were generated by inserting c-myc tag and mRuby3 red fluorescent protein upstream of full length NS1-2 and then subcloning this into the pTagRFP-N vector in place of TagRFP (Epoch Life Sciences, Missouri City, TX). This construct will be referred to as RFP-NS1-2 ...
• Direct DNA sequencing of ‘Candidatus Liberibacter asiaticus’ from Diaphorina citri, the Asian citrus psyllid, and its implications for citrus greening disease management

  Steven Adam Higgins, Marina Mann, Michelle Heck, bioRxiv - Microbiology 2022

  Quote: ... A 450 µl aliquot of the homogenate was then transferred to a sterile 2 ml EconoSpin mini spin column (catalog no. 1910-250, Epoch Life Science, Inc., Sugar Land, TX, USA) containing 450 µl of 70% (v/v ...