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1 - 3 of 3 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019

• Mechanical regulation of cell fate transitions underlying colorectal cancer metastasis formation

  Mirjam C van der Net, et al., bioRxiv - Cancer Biology 2023

  Quote: ... was recloned in a pDONR221 backbone and the Myc-tag was replaced with a Flag-tag (Epoch Life Science). The sequence 5’-CAATGCGGAATATCAATCCCAGCACAGCAAATTCTCCA AAATGTCAGG-3’ was added between base pairs 982 and 983 of the YAP1 gene ...
• Structure of Orthoreovirus RNA Chaperone σNS, a Component of Viral Replication Factories

  Boyang Zhao, et al., bioRxiv - Biochemistry 2023

  Quote: ... and σNS-ΔN17 were subcloned into the bacterial expression vector pET28 with an N-terminal His tag and a TEV protease cleavage site (Epoch Life Science). Escherichia coli DE3 cells (Novagen ...
• Akaby - cell-free protein expression system for linear templates

  Wakana Sato, et al., bioRxiv - Synthetic Biology 2021

  Quote: ... at 37°C for 1 hour and agarose gel-purified with GenCatch Advanced Gel Extraction Kit (Epoch Life Science, Inc., No. 2260050). eGFP fluorescence was measured at λex 488 nm and λem 509 nm with plate reader PMT setting “medium” and 6 reads per well ...