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Citations for Nzytech :
1 - 49 of 49 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... coli cells (NZYTech) grown in LB medium ...
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bioRxiv - Molecular Biology 2022Quote: ... coli NZYStar (NZYTech) and E ...
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bioRxiv - Biophysics 2020Quote: ... coli NZY5α (Nzytech, Portugal), and successful transformants were isolated on LB agar medium containing 10 μg/ml ampicillin ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) Gold competent cells (Nzytech). 300 mL of Luria Bertani medium containing 50 μg/mL kanamycin was inoculated with a single colony of E ...
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bioRxiv - Molecular Biology 2022Quote: ... coli codon-optimized ehcas9 gene supplied by NZYtech was fused to an N-terminal hexahistidine tag under a lac/IPTG-inducible promoter in a pHTP1 vector ...
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bioRxiv - Microbiology 2023Quote: ... All plasmids were transformed into Escherichia coli DH5α (NZYtech) and grown at 37 °C.
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bioRxiv - Immunology 2023Quote: ... coli strain DE3 and grown in NZY auto-induction lysogeny broth (LB) medium (NZYtech, #MB180). Bacteria were harvested by centrifugation and lysed using Cell Disruptor (Constant System ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked with 3% (w/v) BSA (NZYTech), 1xTris Base Solution/0.05% Tween (TBST) ...
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bioRxiv - Molecular Biology 2022Quote: NZYBlue Protein Marker (NZYtech) was used for protein size estimation and protein concentrations were measured with QUBIT® 2.0 (Invitrogen).
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) cells harbouring these various plasmids were individually encapsulated in autoinducible medium (AIM, [NZYtech, Lisboa, Portugal]) supplemented with 50 µg.mL−1 kanamycin using the flow focussing device shown in Supplementary Figure S3C ...
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bioRxiv - Microbiology 2023Quote: ... coli BL21-DE cells containing the respective plasmids were grown on NZY Auto-induction LB medium (NZYTech, Lisbon, Portugal) at 37°C for 20 h ...
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bioRxiv - Molecular Biology 2022Quote: All Gram-negative bacteria used in this work (P. aeruginosa, Escherichia coli, Acinetobacter baumannii, Acinetobacter pitti, Klebsiella pneumoniae) were grown in Lysogeny Broth (LB, NZYTech) at 37°C with aeration (200 rpm shaking) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were transformed into Escherichia coli strain D43 and grown in NZY auto-induction lysogeny broth (LB) medium (NZYtech, #MB180). Bacteria were harvested by centrifugation and lysed using Precellys system according to manufacturer instructions (Bertin Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Gels containing total protein fractions and insoluble protein fractions were stained with blue safe (NZYTech, Cat.MB15201) for 30 minutes and revealed with the Odyssey Infrared Imaging system (Li-cor Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... using NZYColour Protein Marker II (NZYTech). Blotting was performed with an iBlot Gel Transfer System (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were stained with BlueSafe solution (NZYtech). Western blotting on SDS gels was performed with an Invitrogen iBlot2 device and fitting iBlot2 NC stacks (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NZYColour Protein Marker II (NZYTech, Lisbon, Portugal) was used as a protein molecular weight marker ...
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bioRxiv - Biochemistry 2023Quote: ... was stained with BlueSafe protein stain (NZYTech).
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bioRxiv - Microbiology 2019Quote: ... Molecular weights were estimated using a calibration curve (Log10 MW vs Rf) constructed with a prestained protein standard (NZY Colour Protein Marker II, Nzytech, Portugal).
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bioRxiv - Cell Biology 2020Quote: ... Then membranes were incubated in blocking buffer containing 3% BSA (Bovine Serum Albumin, MB04602, Nzytech) in TBS-T for 1h at RT ...
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bioRxiv - Biophysics 2022Quote: ... Protein expression was induced with 1mM of IPTG (Nzytech) and performed for 5 h at 28ºC ...
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bioRxiv - Systems Biology 2023Quote: ... Protein samples diluted in 5x SDS sample buffer (NZYTech) were denatured at 95º for 10 min and resolved in an 8% polyacrylamide gel (SDS-PAGE) ...
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bioRxiv - Biochemistry 2023Quote: ... and visualized by BlueSafe protein stain (NZYTech, ref. MB15201). To further characterize the oligomeric content of TTR1st ...
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bioRxiv - Plant Biology 2021Quote: ... Protein molecular weight marker was NZYColour Marker II (NZYTech, Portugal) or Precision Plus Protein WesternC Standards (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was incubated in 5% (w/v) skimmed milk and 3% (w/v) BSA (NZYTech, Portugal) in phosphate buffered saline containing 0.1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with 2% BSA in PBS (NZYTech) blocking solution for 1h at RT ...
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bioRxiv - Molecular Biology 2019Quote: Protein lysates mixed with 1x SDS-PAGE Sample Loading Buffer (NZYtech) were denatured at 95°C for 10 minutes and centrifuged at 500g for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 18 μl of water and 2 μl TaqαI enzyme (NZYTech) at 10U/ μl ...
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bioRxiv - Cancer Biology 2024Quote: ... Tris-buffered saline with 0.1% Tween 20 was used for washing and antibody incubation as well as membrane blocking with 5% bovine serum albumin (cat. no. MB04602, NZYTech). The chemiluminescent signals were acquired by incubating the membrane with SuperSignal West Femto Maximum Sensitivity Substrate (cat ...
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bioRxiv - Biochemistry 2020Quote: ... the quality and purity of protein batches was assessed by BlueSafe (NZYTech, Lisbon) staining after electrophoresis in SDS-PAGE gels ...
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bioRxiv - Biochemistry 2021Quote: ... the quality and purity of protein batches was assessed by BlueSafe (NZYTech, Lisbon) staining after electrophoresis in SDS-PAGE gels ...
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bioRxiv - Biochemistry 2020Quote: ... the quality and purity of protein batches was assessed by BlueSafe (NZYTech, Lisbon) staining after electrophoresis in SDS-PAGE gels ...
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bioRxiv - Neuroscience 2022Quote: ... Full-length blots with the molecular weight standards (NZYColour Protein Marker I, NZYTech) are provided in Supplementary Figures 5 and 6.
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bioRxiv - Molecular Biology 2022Quote: ... a reaction mixture was prepared with: 2 μl of Buffer A (NZYTech), 12 μl pk1 PCR product ...
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bioRxiv - Biochemistry 2021Quote: ... Clones were visualized after electrophoresis on 2% agarose using GreenSafe Premium (MB13201, NZYTech) on Chemidoc Touch (Biorad) ...
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bioRxiv - Cell Biology 2020Quote: ... Reduction of protein disulfide bonds was carried out with 10 mM dithiothreitol (DTT, Nzytech MB03101) in 100 mM NH4HCO3 and subsequent alkylation was performed with 55 mM iodoacetamide (IAA Sigma I1149-25G) ...
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bioRxiv - Microbiology 2020Quote: ... The libraries were run on 2 % agarose gels stained with GreenSafe (NZYTech, Lisboa, Portugal) and imaged under UV light to verify the library size ...
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bioRxiv - Developmental Biology 2022Quote: ... the coverslips were blocked for 1 h at RT with 2% BSA (NZYTech, MB04602), 2% FBS (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Library sizes were confirmed using 2% agarose gels stained with GreenSafe (NZYTech, Lisboa, Portugal). The libraries were subsequently purified using Mag-Bind RXNPure Plus magnetic beads (Omega Biotek ...
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bioRxiv - Biochemistry 2021Quote: ... Relative gene expression was determined from cDNA using NZYTaq 2× Green Master Mix (MB03903, NZYtech) on MicroAmp Optical 96-well reaction plates (N8010560 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were produced as periplasmic components in 1 L of the NZY Auto-Induction TB medium (NZytech) according to the manufacturer’s recommendations and were purified by immobilized metal affinity chromatography on a HiTrap TALON® crude 1 mL column (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... for 5 min at 95°C in the presence of 50 mM DTT and protein standard (NZYTech, MB09002) was used ...
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bioRxiv - Cell Biology 2021Quote: ... and was further used to amplify RNase2 using NZYTaq II 2×Green master mix (Nzytech, MB358). Genomic DNA was subjected to PCR (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... Digestion with TaqαI was performed with the following reaction mixture: 2 μl of Buffer C (NZYTech), 10 μl of the PCR product ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR product was analysed by electrophoresis on a 2% agarose gel stained with Green Safe Premium (NZYTech).
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bioRxiv - Biochemistry 2020Quote: ... The rest of the plasmids encoding Vav1 mutant proteins were generated in this work by site-directed mutagenesis using the high-fidelity NZYProof DNA polymerase (Cat. #14601, NZYTech) and the appropriate combination of mutation-bearing oligonucleotides (Table S1).
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bioRxiv - Immunology 2021Quote: ... Products derived from PCR were visualized on a 2% agarose/TBE gel with GreenSafe premium nucleic acid stain (NZYTech, Portugal). For primary diagnostics ...
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bioRxiv - Developmental Biology 2023Quote: ... (1) 2 hrs at RT or O/N at 4°C in 75 µg/ml of CBM3a-GFP (CZ00571, Nzytech), (2 ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were visualised using 2 % (w/v) agarose/TBE gels stained with 3 μL Greensafe premium nucleic acid stain (NZYTech, Lisboa, Portugal). TBE gels consisted of 100 mL 1x TBE buffer ...