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Citations for Nzytech :
1 - 33 of 33 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... mk+) supE44 thi-1 recA1 gyrA96 relA1 lac[F′ proA+B+ lacIq ΔlacZM15:Tn10(TcR)]) chemically competent cells (NZYtech) were used as an intermediate host for cloning purposes and they were grow in LB medium at 37 °C under agitation ...
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bioRxiv - Biophysics 2020Quote: ... coli cells (NZYTech) grown in LB medium ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed with NZYol (NZYtech) and RNA extracted using a standard protocol with chloroform-isopropanol-ethanol.
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bioRxiv - Developmental Biology 2023Quote: RNA from FAC-Sorted EdU+ cells (neural cells >650,000) was isolated by NZY Total RNA Isolation kit (NZYTech) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) Gold competent cells (Nzytech). 300 mL of Luria Bertani medium containing 50 μg/mL kanamycin was inoculated with a single colony of E ...
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bioRxiv - Cell Biology 2021Quote: ... and used to transform NZYstar competent cells (NZYtech). The empty pGL3-Basic was used as a negative control and pRL-CMV (Promega ...
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bioRxiv - Microbiology 2023Quote: ... and transformed into NZY5α competent cells (Nzytech MB00402). The presence of the mutation and the absence of undesired mutations were confirmed by Sanger sequencing.
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bioRxiv - Biochemistry 2023Quote: ... DtGH67 and SthAraf62A expressing cells in autoinducible medium (NZYtech) supplemented with 50 µg.ml−1 kanamycin ...
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bioRxiv - Microbiology 2024Quote: ... Assembled products were transformed into NY5α competent cells (NZYtech). Correct insertion was checked by colony PCR using vector-specific primers (Forward ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA from cells was extracted using either NZYol (NZYTech) or GRS Total RNA Kit (GRiSP) ...
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bioRxiv - Cancer Biology 2021Quote: ... After one wash in 1% bovine serum albumin (BSA; NZYtech) in PBS 1x ...
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bioRxiv - Microbiology 2023Quote: ... Isopropyl β-D-1-thiogalactopyranoside (IPTG) was purchased from NZYTech (MB026). The anti-human NF-κB p65 C-terminal domain (c-20 ...
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bioRxiv - Molecular Biology 2020Quote: RNA extracts were obtained from cells using NZY Total RNA Isolation kit (Nzytech) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from HeLa cells using NZYol reagent (NZYTech, Lisboa, Portugal). RNA concentration was determined using the DS-11 Series Spectrophotometer / Fluorometer (DeNovix ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from cells using NZYOL™ RNA Isolation Reagent (NZYTech) and treated with DNase I (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... the cells were transferred to gelrite plates containing kanamycin (100mg/L) (NZYTech, Portugal) and ticarcillin disodium/clavulanate potassium (500mg/L ...
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bioRxiv - Cell Biology 2023Quote: RNA extracts were obtained from cells using NZY Total RNA Isolation kit (Nzytech) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were then blocked with 1,5% bovine serum albumin (BSA, NZYTech, Lisbon, Portugal) for two hours ...
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bioRxiv - Developmental Biology 2022Quote: ... the coverslips were blocked for 1 h at RT with 2% BSA (NZYTech, MB04602), 2% FBS (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: First-strand cDNA was synthesized from 1 μg of total RNA using reverse trancriptase (NZYtech) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) cells harbouring these various plasmids were individually encapsulated in autoinducible medium (AIM, [NZYtech, Lisboa, Portugal]) supplemented with 50 µg.mL−1 kanamycin using the flow focussing device shown in Supplementary Figure S3C ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA (1 μg) was reversely transcribed using the NZYTech Reverse Transcriptase cDNA Synthesis kit (NZYTech, Portugal) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were produced as periplasmic components in 1 L of the NZY Auto-Induction TB medium (NZytech) according to the manufacturer’s recommendations and were purified by immobilized metal affinity chromatography on a HiTrap TALON® crude 1 mL column (Cytiva) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA with NZY First-Strand cDNA Synthesis Kit (NZYTech). Quantitative real-time PCR was performed in a 7500 Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA with NZY-First Strand cDNA Synthesis Kit (NZYTech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... coli BL21-DE cells containing the respective plasmids were grown on NZY Auto-induction LB medium (NZYTech, Lisbon, Portugal) at 37°C for 20 h ...
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bioRxiv - Bioengineering 2020Quote: ... Agarose gels were prepared in TAE buffer (1% w/v) and visualized using GreenSafe® Premium (NZYtech, Portugal). Polyplex formulations were loaded in each well and electrophoresis was carried out for approximately 60 minutes at +90 mV ...
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bioRxiv - Pathology 2023Quote: ... cDNA was obtained by retrotranscription of 500 ng of total RNA from mouse liver or HepG2 cells using NZY First-Strand cDNA synthesis kit (NZYTech). Pah ...
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bioRxiv - Cell Biology 2023Quote: ... For cloning the amplified fragments we used the NZY-A PCR cloning kit and NZYStar Competent Cells (refs. MB053 and MB00501 NZYTech, Portugal). Inserts contained in recombinant plasmids were sequenced as described above using plasmid based primers T7 (TAATACGACTCACTATAGGG ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplification was carried out using primers listed in Supplementary Table 1 (relate to all figures) and Taq polymerase (NZYTech).
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bioRxiv - Developmental Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription into complementary DNA (cDNA) using NZY Reverse Transcriptase enzyme (NZYTech #MB124) and random hexamer mix (NZYTech #MB12901 ...
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bioRxiv - Developmental Biology 2023Quote: ... (1) 2 hrs at RT or O/N at 4°C in 75 µg/ml of CBM3a-GFP (CZ00571, Nzytech), (2 ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized from 1 μg of total RNA by using a NZY First-Strand cDNA Synthesis Kit (NZYTech, Lisbon, Portugal). Quantitative real-time PCR was performed by using assay-on-demand kits (Applied Biosystems) ...