Labshake search
Citations for Nzytech :
1 - 31 of 31 citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... coli cells (NZYTech) grown in LB medium ...
-
bioRxiv - Molecular Biology 2022Quote: ... coli NZYStar (NZYTech) and E ...
-
bioRxiv - Biophysics 2020Quote: ... coli NZY5α (Nzytech, Portugal), and successful transformants were isolated on LB agar medium containing 10 μg/ml ampicillin ...
-
bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) Gold competent cells (Nzytech). 300 mL of Luria Bertani medium containing 50 μg/mL kanamycin was inoculated with a single colony of E ...
-
bioRxiv - Molecular Biology 2022Quote: ... coli codon-optimized ehcas9 gene supplied by NZYtech was fused to an N-terminal hexahistidine tag under a lac/IPTG-inducible promoter in a pHTP1 vector ...
-
bioRxiv - Microbiology 2023Quote: ... All plasmids were transformed into Escherichia coli DH5α (NZYtech) and grown at 37 °C.
-
bioRxiv - Immunology 2023Quote: ... coli strain DE3 and grown in NZY auto-induction lysogeny broth (LB) medium (NZYtech, #MB180). Bacteria were harvested by centrifugation and lysed using Cell Disruptor (Constant System ...
-
bioRxiv - Molecular Biology 2022Quote: NZYBlue Protein Marker (NZYtech) was used for protein size estimation and protein concentrations were measured with QUBIT® 2.0 (Invitrogen).
-
bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) cells harbouring these various plasmids were individually encapsulated in autoinducible medium (AIM, [NZYtech, Lisboa, Portugal]) supplemented with 50 µg.mL−1 kanamycin using the flow focussing device shown in Supplementary Figure S3C ...
-
bioRxiv - Microbiology 2023Quote: ... coli BL21-DE cells containing the respective plasmids were grown on NZY Auto-induction LB medium (NZYTech, Lisbon, Portugal) at 37°C for 20 h ...
-
bioRxiv - Molecular Biology 2022Quote: All Gram-negative bacteria used in this work (P. aeruginosa, Escherichia coli, Acinetobacter baumannii, Acinetobacter pitti, Klebsiella pneumoniae) were grown in Lysogeny Broth (LB, NZYTech) at 37°C with aeration (200 rpm shaking) ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were transformed into Escherichia coli strain D43 and grown in NZY auto-induction lysogeny broth (LB) medium (NZYtech, #MB180). Bacteria were harvested by centrifugation and lysed using Precellys system according to manufacturer instructions (Bertin Technologies ...
-
bioRxiv - Molecular Biology 2023Quote: ... Gels containing total protein fractions and insoluble protein fractions were stained with blue safe (NZYTech, Cat.MB15201) for 30 minutes and revealed with the Odyssey Infrared Imaging system (Li-cor Biosciences).
-
bioRxiv - Cell Biology 2019Quote: ... using NZYColour Protein Marker II (NZYTech). Blotting was performed with an iBlot Gel Transfer System (Invitrogen) ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were stained with BlueSafe solution (NZYtech). Western blotting on SDS gels was performed with an Invitrogen iBlot2 device and fitting iBlot2 NC stacks (Invitrogen) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... NZYColour Protein Marker II (NZYTech, Lisbon, Portugal) was used as a protein molecular weight marker ...
-
bioRxiv - Biochemistry 2023Quote: ... was stained with BlueSafe protein stain (NZYTech).
-
bioRxiv - Microbiology 2019Quote: ... Molecular weights were estimated using a calibration curve (Log10 MW vs Rf) constructed with a prestained protein standard (NZY Colour Protein Marker II, Nzytech, Portugal).
-
bioRxiv - Biophysics 2022Quote: ... Protein expression was induced with 1mM of IPTG (Nzytech) and performed for 5 h at 28ºC ...
-
bioRxiv - Systems Biology 2023Quote: ... Protein samples diluted in 5x SDS sample buffer (NZYTech) were denatured at 95º for 10 min and resolved in an 8% polyacrylamide gel (SDS-PAGE) ...
-
bioRxiv - Biochemistry 2023Quote: ... and visualized by BlueSafe protein stain (NZYTech, ref. MB15201). To further characterize the oligomeric content of TTR1st ...
-
bioRxiv - Plant Biology 2021Quote: ... Protein molecular weight marker was NZYColour Marker II (NZYTech, Portugal) or Precision Plus Protein WesternC Standards (Bio-Rad ...
-
bioRxiv - Molecular Biology 2019Quote: Protein lysates mixed with 1x SDS-PAGE Sample Loading Buffer (NZYtech) were denatured at 95°C for 10 minutes and centrifuged at 500g for 5 minutes ...
-
bioRxiv - Biochemistry 2020Quote: ... the quality and purity of protein batches was assessed by BlueSafe (NZYTech, Lisbon) staining after electrophoresis in SDS-PAGE gels ...
-
bioRxiv - Biochemistry 2021Quote: ... the quality and purity of protein batches was assessed by BlueSafe (NZYTech, Lisbon) staining after electrophoresis in SDS-PAGE gels ...
-
bioRxiv - Biochemistry 2020Quote: ... the quality and purity of protein batches was assessed by BlueSafe (NZYTech, Lisbon) staining after electrophoresis in SDS-PAGE gels ...
-
bioRxiv - Neuroscience 2022Quote: ... Full-length blots with the molecular weight standards (NZYColour Protein Marker I, NZYTech) are provided in Supplementary Figures 5 and 6.
-
bioRxiv - Cell Biology 2020Quote: ... Reduction of protein disulfide bonds was carried out with 10 mM dithiothreitol (DTT, Nzytech MB03101) in 100 mM NH4HCO3 and subsequent alkylation was performed with 55 mM iodoacetamide (IAA Sigma I1149-25G) ...
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were produced as periplasmic components in 1 L of the NZY Auto-Induction TB medium (NZytech) according to the manufacturer’s recommendations and were purified by immobilized metal affinity chromatography on a HiTrap TALON® crude 1 mL column (Cytiva) ...
-
bioRxiv - Cell Biology 2024Quote: ... for 5 min at 95°C in the presence of 50 mM DTT and protein standard (NZYTech, MB09002) was used ...
-
bioRxiv - Biochemistry 2020Quote: ... The rest of the plasmids encoding Vav1 mutant proteins were generated in this work by site-directed mutagenesis using the high-fidelity NZYProof DNA polymerase (Cat. #14601, NZYTech) and the appropriate combination of mutation-bearing oligonucleotides (Table S1).