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Citations for Nzytech :
1 - 43 of 43 citations for 7H Azeto 2 1 b 1 3 dioxino 4 5 e 1 3 oxazin 7 one hexahydro 2 6 dimethyl 2S 4aR 5aS 6S 9aR 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Correct insertion was checked by colony PCR using vector-specific primers (Forward: 5’-GAGAACCCACTGCTTACTGGC-3’; Reverse: 5’-AGGGTCAAGGAAGGCACG-3’) and the NZYTaq II 2x Green Master Mix (NZYtech). Plasmids with correct insertions were checked by Sanger (Eurofins ...
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bioRxiv - Developmental Biology 2023Quote: ... (1) 2 hrs at RT or O/N at 4°C in 75 µg/ml of CBM3a-GFP (CZ00571, Nzytech), (2 ...
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bioRxiv - Cancer Biology 2021Quote: ... After one wash in 1% bovine serum albumin (BSA; NZYtech) in PBS 1x ...
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bioRxiv - Developmental Biology 2022Quote: ... the coverslips were blocked for 1 h at RT with 2% BSA (NZYTech, MB04602), 2% FBS (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... 3% BSA (Nzytech), 0.5% Triton X100 (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 3% BSA (Nzytech), 0.5% triton X100 (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were visualised using 2 % (w/v) agarose/TBE gels stained with 3 μL Greensafe premium nucleic acid stain (NZYTech, Lisboa, Portugal). TBE gels consisted of 100 mL 1x TBE buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 3% BSA (MB04602, Nzytech), 0.5% Triton X100 (T8787 ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was incubated in 5% (w/v) skimmed milk and 3% (w/v) BSA (NZYTech, Portugal) in phosphate buffered saline containing 0.1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... mk+) supE44 thi-1 recA1 gyrA96 relA1 lac[F′ proA+B+ lacIq ΔlacZM15:Tn10(TcR)]) chemically competent cells (NZYtech) were used as an intermediate host for cloning purposes and they were grow in LB medium at 37 °C under agitation ...
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bioRxiv - Neuroscience 2023Quote: ... and 3% bovine serum albumin (BSA, #MB04602, NZYTech) for 1.5h was performed to block nonspecific binding sites ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked with 3% (w/v) BSA (NZYTech), 1xTris Base Solution/0.05% Tween (TBST) ...
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bioRxiv - Cell Biology 2020Quote: Cellulose was labeled with GFP-CBM3a (family 3 carbohydrate binding module; NZYtech, Lisboa, Portugal) following fixation with 1.5% PFA (PFA ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with 2% BSA in PBS (NZYTech) blocking solution for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Then membranes were incubated in blocking buffer containing 3% BSA (Bovine Serum Albumin, MB04602, Nzytech) in TBS-T for 1h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... 18 μl of water and 2 μl TaqαI enzyme (NZYTech) at 10U/ μl ...
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bioRxiv - Molecular Biology 2022Quote: ... a reaction mixture was prepared with: 2 μl of Buffer A (NZYTech), 12 μl pk1 PCR product ...
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bioRxiv - Microbiology 2023Quote: ... Isopropyl β-D-1-thiogalactopyranoside (IPTG) was purchased from NZYTech (MB026). The anti-human NF-κB p65 C-terminal domain (c-20 ...
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bioRxiv - Biochemistry 2021Quote: ... Clones were visualized after electrophoresis on 2% agarose using GreenSafe Premium (MB13201, NZYTech) on Chemidoc Touch (Biorad) ...
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bioRxiv - Microbiology 2020Quote: ... The libraries were run on 2 % agarose gels stained with GreenSafe (NZYTech, Lisboa, Portugal) and imaged under UV light to verify the library size ...
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bioRxiv - Microbiology 2024Quote: ... Library sizes were confirmed using 2% agarose gels stained with GreenSafe (NZYTech, Lisboa, Portugal). The libraries were subsequently purified using Mag-Bind RXNPure Plus magnetic beads (Omega Biotek ...
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bioRxiv - Biochemistry 2021Quote: ... Relative gene expression was determined from cDNA using NZYTaq 2× Green Master Mix (MB03903, NZYtech) on MicroAmp Optical 96-well reaction plates (N8010560 ...
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bioRxiv - Cell Biology 2021Quote: ... and was further used to amplify RNase2 using NZYTaq II 2×Green master mix (Nzytech, MB358). Genomic DNA was subjected to PCR (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... Digestion with TaqαI was performed with the following reaction mixture: 2 μl of Buffer C (NZYTech), 10 μl of the PCR product ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR product was analysed by electrophoresis on a 2% agarose gel stained with Green Safe Premium (NZYTech).
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bioRxiv - Molecular Biology 2019Quote: First-strand cDNA was synthesized from 1 μg of total RNA using reverse trancriptase (NZYtech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA (1 μg) was reversely transcribed using the NZYTech Reverse Transcriptase cDNA Synthesis kit (NZYTech, Portugal) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were produced as periplasmic components in 1 L of the NZY Auto-Induction TB medium (NZytech) according to the manufacturer’s recommendations and were purified by immobilized metal affinity chromatography on a HiTrap TALON® crude 1 mL column (Cytiva) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA with NZY First-Strand cDNA Synthesis Kit (NZYTech). Quantitative real-time PCR was performed in a 7500 Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA with NZY-First Strand cDNA Synthesis Kit (NZYTech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Products derived from PCR were visualized on a 2% agarose/TBE gel with GreenSafe premium nucleic acid stain (NZYTech, Portugal). For primary diagnostics ...
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bioRxiv - Bioengineering 2020Quote: ... Agarose gels were prepared in TAE buffer (1% w/v) and visualized using GreenSafe® Premium (NZYtech, Portugal). Polyplex formulations were loaded in each well and electrophoresis was carried out for approximately 60 minutes at +90 mV ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplification was carried out using primers listed in Supplementary Table 1 (relate to all figures) and Taq polymerase (NZYTech).
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bioRxiv - Developmental Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription into complementary DNA (cDNA) using NZY Reverse Transcriptase enzyme (NZYTech #MB124) and random hexamer mix (NZYTech #MB12901 ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized from 1 μg of total RNA by using a NZY First-Strand cDNA Synthesis Kit (NZYTech, Lisbon, Portugal). Quantitative real-time PCR was performed by using assay-on-demand kits (Applied Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: Cardosin B coding sequence (EMBL no. AJ237674) without the native signal peptide was synthesized by NZYTech (NZYTech, Portugal) with the addition of NcoI and SalI restriction sites at the 5’and 3’ends respectively ...
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bioRxiv - Neuroscience 2021Quote: ... in TBS-T or 5%BSA (NZYTech) in TBS-T for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... from 10 ng of RNA was performed using the One-step NZYTECH RT-qPCR Green kit according to manufactureŕs protocol (NZYTECH, MB34302). RT-PCR was normalized using the housekeeping genes hypoxanthine phosphoribosyl-transferase 1 (HPRT1 ...
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bioRxiv - Neuroscience 2024Quote: ... and bovine serum albumin 5% (BSA, MB04602; NZYtech) in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... for 5 min at 95°C in the presence of 50 mM DTT and protein standard (NZYTech, MB09002) was used ...
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bioRxiv - Evolutionary Biology 2024Quote: For sequencing the genome and 5’ ends of JUv2572_vlc RNA was extracted using the Viral RNA Isolation Kit (NZYTech) following manufacturer instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Tris-buffered saline with 0.1% Tween 20 was used for washing and antibody incubation as well as membrane blocking with 5% bovine serum albumin (cat. no. MB04602, NZYTech). The chemiluminescent signals were acquired by incubating the membrane with SuperSignal West Femto Maximum Sensitivity Substrate (cat ...
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bioRxiv - Cell Biology 2023Quote: ... 1-5 μg total RNA was used for cDNA synthesis using the NZY First-strand cDNA Synthesis Kit (ref. MB40001, NZYTech, Portugal), primed with a mix of oligo (dT)18 to enrich the samples with cDNA from mature mRNAs and hexamers ...