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Citations for Nzytech :
1 - 37 of 37 citations for 7 Chloro 3 4 2 hydroxyethyl 1 piperazinyl 1 2 propoxyethyl pyrido 3 4 b pyrazin 2 1H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... (1) 2 hrs at RT or O/N at 4°C in 75 µg/ml of CBM3a-GFP (CZ00571, Nzytech), (2 ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were visualised using 2 % (w/v) agarose/TBE gels stained with 3 μL Greensafe premium nucleic acid stain (NZYTech, Lisboa, Portugal). TBE gels consisted of 100 mL 1x TBE buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... the coverslips were blocked for 1 h at RT with 2% BSA (NZYTech, MB04602), 2% FBS (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... 3% BSA (Nzytech), 0.5% Triton X100 (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 3% BSA (Nzytech), 0.5% triton X100 (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with 2% BSA in PBS (NZYTech) blocking solution for 1h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 3% BSA (MB04602, Nzytech), 0.5% Triton X100 (T8787 ...
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bioRxiv - Molecular Biology 2022Quote: ... 18 μl of water and 2 μl TaqαI enzyme (NZYTech) at 10U/ μl ...
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bioRxiv - Molecular Biology 2022Quote: ... a reaction mixture was prepared with: 2 μl of Buffer A (NZYTech), 12 μl pk1 PCR product ...
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bioRxiv - Cancer Biology 2021Quote: ... After one wash in 1% bovine serum albumin (BSA; NZYtech) in PBS 1x ...
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bioRxiv - Biochemistry 2021Quote: ... Clones were visualized after electrophoresis on 2% agarose using GreenSafe Premium (MB13201, NZYTech) on Chemidoc Touch (Biorad) ...
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bioRxiv - Microbiology 2020Quote: ... The libraries were run on 2 % agarose gels stained with GreenSafe (NZYTech, Lisboa, Portugal) and imaged under UV light to verify the library size ...
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bioRxiv - Microbiology 2024Quote: ... Library sizes were confirmed using 2% agarose gels stained with GreenSafe (NZYTech, Lisboa, Portugal). The libraries were subsequently purified using Mag-Bind RXNPure Plus magnetic beads (Omega Biotek ...
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bioRxiv - Microbiology 2024Quote: ... Correct insertion was checked by colony PCR using vector-specific primers (Forward: 5’-GAGAACCCACTGCTTACTGGC-3’; Reverse: 5’-AGGGTCAAGGAAGGCACG-3’) and the NZYTaq II 2x Green Master Mix (NZYtech). Plasmids with correct insertions were checked by Sanger (Eurofins ...
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bioRxiv - Neuroscience 2023Quote: ... and 3% bovine serum albumin (BSA, #MB04602, NZYTech) for 1.5h was performed to block nonspecific binding sites ...
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bioRxiv - Biochemistry 2021Quote: ... Relative gene expression was determined from cDNA using NZYTaq 2× Green Master Mix (MB03903, NZYtech) on MicroAmp Optical 96-well reaction plates (N8010560 ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked with 3% (w/v) BSA (NZYTech), 1xTris Base Solution/0.05% Tween (TBST) ...
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bioRxiv - Cell Biology 2021Quote: ... and was further used to amplify RNase2 using NZYTaq II 2×Green master mix (Nzytech, MB358). Genomic DNA was subjected to PCR (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... Digestion with TaqαI was performed with the following reaction mixture: 2 μl of Buffer C (NZYTech), 10 μl of the PCR product ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR product was analysed by electrophoresis on a 2% agarose gel stained with Green Safe Premium (NZYTech).
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bioRxiv - Immunology 2021Quote: ... Products derived from PCR were visualized on a 2% agarose/TBE gel with GreenSafe premium nucleic acid stain (NZYTech, Portugal). For primary diagnostics ...
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bioRxiv - Microbiology 2023Quote: ... mk+) supE44 thi-1 recA1 gyrA96 relA1 lac[F′ proA+B+ lacIq ΔlacZM15:Tn10(TcR)]) chemically competent cells (NZYtech) were used as an intermediate host for cloning purposes and they were grow in LB medium at 37 °C under agitation ...
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bioRxiv - Cell Biology 2020Quote: Cellulose was labeled with GFP-CBM3a (family 3 carbohydrate binding module; NZYtech, Lisboa, Portugal) following fixation with 1.5% PFA (PFA ...
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bioRxiv - Cell Biology 2020Quote: ... Then membranes were incubated in blocking buffer containing 3% BSA (Bovine Serum Albumin, MB04602, Nzytech) in TBS-T for 1h at RT ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was incubated in 5% (w/v) skimmed milk and 3% (w/v) BSA (NZYTech, Portugal) in phosphate buffered saline containing 0.1% (v/v ...
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bioRxiv - Plant Biology 2021Quote: Cardosin B coding sequence (EMBL no. AJ237674) without the native signal peptide was synthesized by NZYTech (NZYTech, Portugal) with the addition of NcoI and SalI restriction sites at the 5’and 3’ends respectively ...
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bioRxiv - Microbiology 2023Quote: ... Isopropyl β-D-1-thiogalactopyranoside (IPTG) was purchased from NZYTech (MB026). The anti-human NF-κB p65 C-terminal domain (c-20 ...
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bioRxiv - Neuroscience 2024Quote: ... from 10 ng of RNA was performed using the One-step NZYTECH RT-qPCR Green kit according to manufactureŕs protocol (NZYTECH, MB34302). RT-PCR was normalized using the housekeeping genes hypoxanthine phosphoribosyl-transferase 1 (HPRT1 ...
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bioRxiv - Molecular Biology 2019Quote: First-strand cDNA was synthesized from 1 μg of total RNA using reverse trancriptase (NZYtech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA (1 μg) was reversely transcribed using the NZYTech Reverse Transcriptase cDNA Synthesis kit (NZYTech, Portugal) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were produced as periplasmic components in 1 L of the NZY Auto-Induction TB medium (NZytech) according to the manufacturer’s recommendations and were purified by immobilized metal affinity chromatography on a HiTrap TALON® crude 1 mL column (Cytiva) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA with NZY First-Strand cDNA Synthesis Kit (NZYTech). Quantitative real-time PCR was performed in a 7500 Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA with NZY-First Strand cDNA Synthesis Kit (NZYTech) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Agarose gels were prepared in TAE buffer (1% w/v) and visualized using GreenSafe® Premium (NZYtech, Portugal). Polyplex formulations were loaded in each well and electrophoresis was carried out for approximately 60 minutes at +90 mV ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplification was carried out using primers listed in Supplementary Table 1 (relate to all figures) and Taq polymerase (NZYTech).
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bioRxiv - Developmental Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription into complementary DNA (cDNA) using NZY Reverse Transcriptase enzyme (NZYTech #MB124) and random hexamer mix (NZYTech #MB12901 ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized from 1 μg of total RNA by using a NZY First-Strand cDNA Synthesis Kit (NZYTech, Lisbon, Portugal). Quantitative real-time PCR was performed by using assay-on-demand kits (Applied Biosystems) ...