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Citations for Nzytech :
1 - 34 of 34 citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Products derived from PCR were visualized on a 2% agarose/TBE gel with GreenSafe premium nucleic acid stain (NZYTech, Portugal). For primary diagnostics ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were visualised using 2 % (w/v) agarose/TBE gels stained with 3 μL Greensafe premium nucleic acid stain (NZYTech, Lisboa, Portugal). TBE gels consisted of 100 mL 1x TBE buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... the coverslips were blocked for 1 h at RT with 2% BSA (NZYTech, MB04602), 2% FBS (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... (1) 2 hrs at RT or O/N at 4°C in 75 µg/ml of CBM3a-GFP (CZ00571, Nzytech), (2 ...
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bioRxiv - Neuroscience 2021Quote: ... in TBS-T or 5%BSA (NZYTech) in TBS-T for 1 h at RT ...
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bioRxiv - Microbiology 2024Quote: ... Correct insertion was checked by colony PCR using vector-specific primers (Forward: 5’-GAGAACCCACTGCTTACTGGC-3’; Reverse: 5’-AGGGTCAAGGAAGGCACG-3’) and the NZYTaq II 2x Green Master Mix (NZYtech). Plasmids with correct insertions were checked by Sanger (Eurofins ...
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bioRxiv - Neuroscience 2024Quote: ... and bovine serum albumin 5% (BSA, MB04602; NZYtech) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with 2% BSA in PBS (NZYTech) blocking solution for 1h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... 18 μl of water and 2 μl TaqαI enzyme (NZYTech) at 10U/ μl ...
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bioRxiv - Molecular Biology 2022Quote: ... a reaction mixture was prepared with: 2 μl of Buffer A (NZYTech), 12 μl pk1 PCR product ...
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bioRxiv - Biochemistry 2021Quote: ... Clones were visualized after electrophoresis on 2% agarose using GreenSafe Premium (MB13201, NZYTech) on Chemidoc Touch (Biorad) ...
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bioRxiv - Microbiology 2020Quote: ... The libraries were run on 2 % agarose gels stained with GreenSafe (NZYTech, Lisboa, Portugal) and imaged under UV light to verify the library size ...
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bioRxiv - Microbiology 2024Quote: ... Library sizes were confirmed using 2% agarose gels stained with GreenSafe (NZYTech, Lisboa, Portugal). The libraries were subsequently purified using Mag-Bind RXNPure Plus magnetic beads (Omega Biotek ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was incubated in 5% (w/v) skimmed milk and 3% (w/v) BSA (NZYTech, Portugal) in phosphate buffered saline containing 0.1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... Relative gene expression was determined from cDNA using NZYTaq 2× Green Master Mix (MB03903, NZYtech) on MicroAmp Optical 96-well reaction plates (N8010560 ...
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bioRxiv - Cell Biology 2024Quote: ... for 5 min at 95°C in the presence of 50 mM DTT and protein standard (NZYTech, MB09002) was used ...
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bioRxiv - Cell Biology 2021Quote: ... and was further used to amplify RNase2 using NZYTaq II 2×Green master mix (Nzytech, MB358). Genomic DNA was subjected to PCR (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... Digestion with TaqαI was performed with the following reaction mixture: 2 μl of Buffer C (NZYTech), 10 μl of the PCR product ...
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bioRxiv - Evolutionary Biology 2024Quote: For sequencing the genome and 5’ ends of JUv2572_vlc RNA was extracted using the Viral RNA Isolation Kit (NZYTech) following manufacturer instructions.
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bioRxiv - Molecular Biology 2022Quote: ... PCR product was analysed by electrophoresis on a 2% agarose gel stained with Green Safe Premium (NZYTech).
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bioRxiv - Cancer Biology 2024Quote: ... Tris-buffered saline with 0.1% Tween 20 was used for washing and antibody incubation as well as membrane blocking with 5% bovine serum albumin (cat. no. MB04602, NZYTech). The chemiluminescent signals were acquired by incubating the membrane with SuperSignal West Femto Maximum Sensitivity Substrate (cat ...
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bioRxiv - Cell Biology 2023Quote: ... 1-5 μg total RNA was used for cDNA synthesis using the NZY First-strand cDNA Synthesis Kit (ref. MB40001, NZYTech, Portugal), primed with a mix of oligo (dT)18 to enrich the samples with cDNA from mature mRNAs and hexamers ...
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bioRxiv - Cancer Biology 2021Quote: ... After one wash in 1% bovine serum albumin (BSA; NZYtech) in PBS 1x ...
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bioRxiv - Microbiology 2023Quote: ... Isopropyl β-D-1-thiogalactopyranoside (IPTG) was purchased from NZYTech (MB026). The anti-human NF-κB p65 C-terminal domain (c-20 ...
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bioRxiv - Molecular Biology 2019Quote: First-strand cDNA was synthesized from 1 μg of total RNA using reverse trancriptase (NZYtech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA (1 μg) was reversely transcribed using the NZYTech Reverse Transcriptase cDNA Synthesis kit (NZYTech, Portugal) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were produced as periplasmic components in 1 L of the NZY Auto-Induction TB medium (NZytech) according to the manufacturer’s recommendations and were purified by immobilized metal affinity chromatography on a HiTrap TALON® crude 1 mL column (Cytiva) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA with NZY First-Strand cDNA Synthesis Kit (NZYTech). Quantitative real-time PCR was performed in a 7500 Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA with NZY-First Strand cDNA Synthesis Kit (NZYTech) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Agarose gels were prepared in TAE buffer (1% w/v) and visualized using GreenSafe® Premium (NZYtech, Portugal). Polyplex formulations were loaded in each well and electrophoresis was carried out for approximately 60 minutes at +90 mV ...
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bioRxiv - Microbiology 2023Quote: ... mk+) supE44 thi-1 recA1 gyrA96 relA1 lac[F′ proA+B+ lacIq ΔlacZM15:Tn10(TcR)]) chemically competent cells (NZYtech) were used as an intermediate host for cloning purposes and they were grow in LB medium at 37 °C under agitation ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplification was carried out using primers listed in Supplementary Table 1 (relate to all figures) and Taq polymerase (NZYTech).
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bioRxiv - Developmental Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription into complementary DNA (cDNA) using NZY Reverse Transcriptase enzyme (NZYTech #MB124) and random hexamer mix (NZYTech #MB12901 ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized from 1 μg of total RNA by using a NZY First-Strand cDNA Synthesis Kit (NZYTech, Lisbon, Portugal). Quantitative real-time PCR was performed by using assay-on-demand kits (Applied Biosystems) ...