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Citations for Boston Biochem :
1 - 41 of 41 citations for Recombinant E. coli LT IIa His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 buffer (10% sucrose ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged human recombinant ubiquitin (Boston Biochem) was added to egg extracts at a final concentration of 0.1 μg/μL unless otherwise stated ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant human UBE1 (E-305, Boston Biochem), recombinant human UbcH5a/UBE2D1 (E2-616 ...
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bioRxiv - Biochemistry 2021Quote: USP8 immunoprecipitates or His-tagged FRIP8 precipitates were incubated with 1-μM ubiquitin-VME (Boston Biochem) in TBS supplemented with 1-mM DTT at 37°C for 5 min or 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... translations were performed in the presence of 10 μM HA- or His-tagged Ubiquitin (Boston Biochem, USA). Denaturing immunoprecipitation of the ubiquitinated species was performed as previously described (Yanagitani et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant human BAP1-FL-WT protein was purchased from Boston Biochem (E-345-050). The Duet-His-ULD/AB protein complex was purified under native purification conditions using Cobalt beads (Talon) ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged ubiquitin mutants (Boston Biochem) were added at final concentrations of 1 µg/µL ...
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bioRxiv - Plant Biology 2021Quote: ... 1.25 µg Flag tagged ubiquitin (Boston Biochem), 2 µg purified GST-PUB25 and PUB26 or HIS-PUB26 were added to 30 µL of ubiquitination reaction buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... and STAMBPL1 (Boston Biochem #E-551). Reactions were measured with similar conditions as were used for measuring STAMBPJAMM inhibition ...
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bioRxiv - Biochemistry 2021Quote: ... inhibition was evaluated with full-length STAMBP (Boston Biochem #E-549) (activated with STAM1 (Boston Biochem #E-550)) and STAMBPL1 (Boston Biochem #E-551) ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.85 µM UBE1 (Boston Biochem E-304-050), 5 mM adenosine triphosphate ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.3 µM UBE1 (Boston Biochem E-304-050), 1.5 µM UBE2T (Boston Biochem E2-695) ...
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bioRxiv - Neuroscience 2019Quote: ... 1 µM His-Ubiquitin (Boston Biochem, Cat#U-530), 50 nM mouse E1 enzyme ...
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bioRxiv - Cell Biology 2023Quote: ... UbcH5c and His-ubiquitin were purchased from Boston Biochem. E1 (50 nM) ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mg His-ubiquitin (BB-U-530, Boston Biochem), 2 mg purified MBP-KEG fusion protein (as E3 enzyme) ...
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bioRxiv - Microbiology 2023Quote: ... 80 nM recombinant human E1 (Boston Biochem), 400 nM recombinant human E2 enzymes (Boston Biochem ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.1 μg of UBE1 (Boston Biochem. Inc, E-305), 0.1 μg UBE2D1 (Boston Bichem ...
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bioRxiv - Cell Biology 2020Quote: ... 0.2 nM (#E-582 residues 57-517, Boston Biochem) was added and the plates pre-incubated for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 μg of UBE1 (Boston Biochem. Inc., E-305), 0.1 μg UBE2D1 (Boston Bichem ...
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bioRxiv - Cell Biology 2023Quote: ... 225 nM E1-UBE-1 (Boston Biochem, E-305), 1.25 μM E2-UbcH5b (Boston Biochem ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant human UbcH5a/UBE2D1 (E2-616, Boston Biochem), Myc-ubiquitin (U-115 ...
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bioRxiv - Neuroscience 2024Quote: Recombinant p-S65-PRKN (Boston Biochem, E3-166), non-phosphorylated PRKN (Boston Biochem ...
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bioRxiv - Microbiology 2023Quote: ... 400 nM recombinant human E2 enzymes (Boston Biochem) and 400 nM purified E3 ligases in 50 mM Tris-Cl (pH 7.5) ...
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bioRxiv - Immunology 2021Quote: ... E3 (0.5 μM, 6 μl, His-cIAP1, Boston Biochem, E3-280) and 15 μl of purified FLAG-IL-1β in Ubiquitin assay buffer containing 2 mM ATP (total volume of 30 μl) ...
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bioRxiv - Biochemistry 2021Quote: ... inhibition was evaluated with full-length STAMBP (Boston Biochem #E-549) (activated with STAM1 (Boston Biochem #E-550) ...
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bioRxiv - Biochemistry 2023Quote: ... UBE1 (0.4 μL, 5 μM, Boston Biochem. Inc., E-305-025), UBE2D1 (5.2 μL ...
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bioRxiv - Biochemistry 2023Quote: ... UBE1 (0.2 μL, 5 μM, Boston Biochem. Inc., E-305-025), UBE2D1 (2.6 μL ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... Ubiquitin (U-100H) and recombinant DUBs were provided by Boston Biochem (E-519 ...
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bioRxiv - Biochemistry 2020Quote: ... Pure recombinant human RNF114 was purchased from Boston Biochem (K-220). RNF114 (0.25 μg ...
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bioRxiv - Molecular Biology 2023Quote: ... in the presence of 5 nM of 20S proteasome (Boston Biochem, E-360), at 37°C ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... Ubiquitin vinyl sulfone tagged with HA (U-212) and HA-ubiquitin (U-110) purchased from Boston Biochem. For immunoprecipitation anti-HA-Agarose beads (A2095 ...
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bioRxiv - Microbiology 2023Quote: ... reaction mixtures (12.5 μl) containing 5 μg of recombinant human Ub (Boston Biochem), 80 nM recombinant human E1 (Boston Biochem) ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 nM GST-tagged Ubiquitin-activating enzyme E1 and 250 nM Ubiquitin-conjugating enzyme E2 UbcH5 [both from Boston Biochem, USA]) ...
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bioRxiv - Microbiology 2021Quote: ... The SCFFBW7 E3 ligase complex was incubated with recombinant 0.1 mM E1 (UBE1; Boston Biochem), 0.25 mM E2 (cdc34 ...
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bioRxiv - Biochemistry 2021Quote: ... Boc-L-R-R-AMC (trypsin-like activity) and Z-L-L-E-AMC (caspase-like activity) (Boston Biochem). The proteasome samples were incubated with 50 μM AMC-peptide in the respective purification buffers (standard or the exogenous nucleotide depleted buffer ...
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bioRxiv - Cell Biology 2019Quote: Immunopurified FLAG-BIRC6 on beads was incubated with the reaction mixture containing 125 nM GST-UBA6 (Boston Biochem, E-307), 6 μM 6xHis-LC3B (ProSpec Bio ...
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bioRxiv - Cell Biology 2024Quote: The in vitro ubiquitination reaction was performed in a 30 μl mixture containing 200 ng E1 enzyme (BB-E-304-050, Boston Biochem), 200 ng E2 enzyme (BB-E2-616-100 ...
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bioRxiv - Molecular Biology 2019Quote: ... UBE2TC86K was conjugated to ubiquitin through an isopeptide bond in a 200 µL reaction containing 125 µM His-Ubiquitin (Boston Biochem U-530), 125 µM UBE2TC86K ...
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bioRxiv - Biochemistry 2022Quote: The ubiquitination assays were performed in a 25 μl reaction volume with the following components: 100 nM UBE1 (Boston Biochem E-304), 1.5 μM UBCH5C (Boston Biochem E2-627) ...
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bioRxiv - Cell Biology 2024Quote: ... The DUBs used in this study are USP2 (Enzo, BML-UW9850-0100, 1 µM and Boston Biochem, E-504-0650, 1 µM), vOTU (1 µM) ...
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bioRxiv - Microbiology 2023Quote: ... each 25 μl pelleted resin was resuspended with 4μg of recombinant human homotypic-linked polyubiquitin chains Ub3-7 (K63 or K48, 4μg/600μl reaction volume, Boston Biochem, R&D) and 500μM ε-NAD in 1X ART buffer for 2 hrs with gentle end-over-end rotation at 37°C ...