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Citations for Boston Biochem :
1 - 35 of 35 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Sf 21 (baculovirus)-derived human USP30 protein) (10 nM; final assay concentration = 5 nM; Boston Biochem) was prepared in USP30 activity assay buffer (50 mM Tris base ...
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bioRxiv - Synthetic Biology 2021Quote: ... 50 μM human ubiquitin (Boston Biochem), 4 mM ATP and 1 mM DTT in 20 mM MOPs ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 mM human ubiquitin (Boston Biochem) and 2 mM ATP (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 20 µM human ubiquitin (Boston Biochem) and 5 mM ATP as indicated in 40 µL ubiquitination assay buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant human UBE1 (E-305, Boston Biochem), recombinant human UbcH5a/UBE2D1 (E2-616 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 4 μM human UbcH5α/UBE2D1 (Boston Biochem), 3 μM uAb (or equivalent control protein) ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged human recombinant ubiquitin (Boston Biochem) was added to egg extracts at a final concentration of 0.1 μg/μL unless otherwise stated ...
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bioRxiv - Microbiology 2023Quote: ... 80 nM recombinant human E1 (Boston Biochem), 400 nM recombinant human E2 enzymes (Boston Biochem ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant human UbcH5a/UBE2D1 (E2-616, Boston Biochem), Myc-ubiquitin (U-115 ...
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bioRxiv - Microbiology 2020Quote: ... 100 µM of human wild-type (Boston Biochem) or K63R ubiquitin (2B Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... or human His6-ubiquitin (Boston Biochem, U-530), mouse E1 (Ube1 ...
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bioRxiv - Microbiology 2023Quote: ... 400 nM recombinant human E2 enzymes (Boston Biochem) and 400 nM purified E3 ligases in 50 mM Tris-Cl (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... human proteasomes were treated with ubiquitin-vinyl-sulfone (UbVS, Boston Biochem). UbVS was added to the resin at 1-1.5 mM ...
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bioRxiv - Biochemistry 2020Quote: ... Pure recombinant human RNF114 was purchased from Boston Biochem (K-220). RNF114 (0.25 μg ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human ISG15−7-amino-4-methylcourmarin (ISG15-AMC) was purchased from Boston Biochem. Lys48 linked tetra-Ub were obtained from Boston Biochem ...
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bioRxiv - Microbiology 2023Quote: ... reaction mixtures (12.5 μl) containing 5 μg of recombinant human Ub (Boston Biochem), 80 nM recombinant human E1 (Boston Biochem) ...
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bioRxiv - Plant Biology 2021Quote: ... 10 μg unlabeled ubiquitin (Boston Biochem), and 2 μg MBP-COP1 (previously incubated with 20 μM ZnCl2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant human BAP1-FL-WT protein was purchased from Boston Biochem (E-345-050). The Duet-His-ULD/AB protein complex was purified under native purification conditions using Cobalt beads (Talon) ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg of Myc-ubiquitin (Boston Biochem), 1 μM ubiquitin aldehyde (Boston Biochem) ...
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bioRxiv - Cell Biology 2021Quote: ... energy regeneration solution (B-10, Boston Biochem) in assay buffer were carefully pipetted directly on the arrays ...
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bioRxiv - Biophysics 2024Quote: ... We added 10 μL of the enzyme:peptide mixture to 10 μL of 250 nM Ub-AMC (7-amino-4-methylcoumarin) (Boston Biochem). DUB activity was measured by monitoring the increase in AMC fluorescence in a plate reader ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μM HA-Ub (Boston Biochem, U-110), and 10 μM MgATP (Boston Biochem ...
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bioRxiv - Cell Biology 2019Quote: ... and 10 μM MgATP (Boston Biochem, B-20), in a final volume of 30 μl of E3 ligase buffer (Boston Biochem ...
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bioRxiv - Cell Biology 2019Quote: ... 200 nM BI2536 and 10 µM MG132 (Boston Biochem). Cycloheximide (CHX ...
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bioRxiv - Synthetic Biology 2021Quote: Ubiquitination assays were performed as previously described 45 in the presence of 0.1 μM purified human UBE1 (Boston Biochem), 4 μM human UbcH5α/UBE2D1 (Boston Biochem) ...
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bioRxiv - Microbiology 2021Quote: The selectivity of the most potent inhibitors was tested against the human deubiquitinating enzymes USP7 and USP14 (Boston Biochem). Assay conditions were similar to the PLpro primary assay ...
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bioRxiv - Cell Biology 2021Quote: ... the bait GST or GST fusion protein was incubated with purified human 26S proteasome (E365; Boston Biochem, Cambridge, MA, USA) at 4 °C for 2 h in binding buffer (1× PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant human ubiquitin, His6-ubiquitin, and ubiquitin KR mutants (K6R, K11R, K27R, K29R, K33R, K48R, K63R, K0) were purchased from Boston Biochem.
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bioRxiv - Microbiology 2023Quote: ... each 25 μl pelleted resin was resuspended with 4μg of recombinant human homotypic-linked polyubiquitin chains Ub3-7 (K63 or K48, 4μg/600μl reaction volume, Boston Biochem, R&D) and 500μM ε-NAD in 1X ART buffer for 2 hrs with gentle end-over-end rotation at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of 10 mg/ml Ub solution (Boston Biochem; #U-100H), and 1μl radiolabeled in vitro translated protein of interest ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of 10 mg/ml Ubiquitin (Ub) solution (Boston Biochem; #U-100H) and 1 µl radiolabeled IVT product of interest ...
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bioRxiv - Biochemistry 2021Quote: ... were charged with 10 µg ubiquitin or N-terminally fluorescein-labelled Ub (FluoUb, Boston Biochem) using 0.02 µg ubiquitin-activating enzyme 1 (His6-E1 ...
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bioRxiv - Biochemistry 2022Quote: ... 0.16 μM myc-Cyclin D1-CDK4 complex and 10 mM MgATP solution (Boston Biochem B-20) in E3 ligase reaction buffer (Boston Biochem B-71) ...
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bioRxiv - Molecular Biology 2020Quote: ... translations were performed in the presence of 10 μM HA- or His-tagged Ubiquitin (Boston Biochem, USA). Denaturing immunoprecipitation of the ubiquitinated species was performed as previously described (Yanagitani et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 mM Na3VO4) at rt for 5-10 min before addition of master mix (42 pmol E1 (UBE1, Boston Biochem or Ubiquitin-Proteasome Biotechnologies) ...