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Citations for Boston Biochem :
1 - 46 of 46 citations for Human Eukaryotic Translation Termination Factor 1 ETF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... translations were performed in the presence of 10 μM HA- or His-tagged Ubiquitin (Boston Biochem, USA). Denaturing immunoprecipitation of the ubiquitinated species was performed as previously described (Yanagitani et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... 50 μM human ubiquitin (Boston Biochem), 4 mM ATP and 1 mM DTT in 20 mM MOPs ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 mM human ubiquitin (Boston Biochem) and 2 mM ATP (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 20 µM human ubiquitin (Boston Biochem) and 5 mM ATP as indicated in 40 µL ubiquitination assay buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant human UBE1 (E-305, Boston Biochem), recombinant human UbcH5a/UBE2D1 (E2-616 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 4 μM human UbcH5α/UBE2D1 (Boston Biochem), 3 μM uAb (or equivalent control protein) ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged human recombinant ubiquitin (Boston Biochem) was added to egg extracts at a final concentration of 0.1 μg/μL unless otherwise stated ...
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bioRxiv - Microbiology 2023Quote: ... 80 nM recombinant human E1 (Boston Biochem), 400 nM recombinant human E2 enzymes (Boston Biochem ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant human UbcH5a/UBE2D1 (E2-616, Boston Biochem), Myc-ubiquitin (U-115 ...
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bioRxiv - Microbiology 2020Quote: ... 100 µM of human wild-type (Boston Biochem) or K63R ubiquitin (2B Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... or human His6-ubiquitin (Boston Biochem, U-530), mouse E1 (Ube1 ...
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bioRxiv - Microbiology 2023Quote: ... 400 nM recombinant human E2 enzymes (Boston Biochem) and 400 nM purified E3 ligases in 50 mM Tris-Cl (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... human proteasomes were treated with ubiquitin-vinyl-sulfone (UbVS, Boston Biochem). UbVS was added to the resin at 1-1.5 mM ...
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bioRxiv - Biochemistry 2020Quote: ... Pure recombinant human RNF114 was purchased from Boston Biochem (K-220). RNF114 (0.25 μg ...
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bioRxiv - Neuroscience 2024Quote: ... the supernatant of each single B cell well was screened for antigen specificity through direct ELISA for targeting full-length monomeric p-S65-Ub protein (Boston Biochem, U-102), free p-S65-Ub peptides 1 and 2 and BSA-conjugated p-S65-Ub peptides 1 and 2 (provided by 21st Century Biochemicals) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human ISG15−7-amino-4-methylcourmarin (ISG15-AMC) was purchased from Boston Biochem. Lys48 linked tetra-Ub were obtained from Boston Biochem ...
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bioRxiv - Microbiology 2023Quote: ... reaction mixtures (12.5 μl) containing 5 μg of recombinant human Ub (Boston Biochem), 80 nM recombinant human E1 (Boston Biochem) ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant human BAP1-FL-WT protein was purchased from Boston Biochem (E-345-050). The Duet-His-ULD/AB protein complex was purified under native purification conditions using Cobalt beads (Talon) ...
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bioRxiv - Neuroscience 2021Quote: ... Sf 21 (baculovirus)-derived human USP30 protein) (10 nM; final assay concentration = 5 nM; Boston Biochem) was prepared in USP30 activity assay buffer (50 mM Tris base ...
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bioRxiv - Synthetic Biology 2021Quote: Ubiquitination assays were performed as previously described 45 in the presence of 0.1 μM purified human UBE1 (Boston Biochem), 4 μM human UbcH5α/UBE2D1 (Boston Biochem) ...
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bioRxiv - Microbiology 2021Quote: The selectivity of the most potent inhibitors was tested against the human deubiquitinating enzymes USP7 and USP14 (Boston Biochem). Assay conditions were similar to the PLpro primary assay ...
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bioRxiv - Cell Biology 2021Quote: ... the bait GST or GST fusion protein was incubated with purified human 26S proteasome (E365; Boston Biochem, Cambridge, MA, USA) at 4 °C for 2 h in binding buffer (1× PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant human ubiquitin, His6-ubiquitin, and ubiquitin KR mutants (K6R, K11R, K27R, K29R, K33R, K48R, K63R, K0) were purchased from Boston Biochem.
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bioRxiv - Microbiology 2023Quote: ... each 25 μl pelleted resin was resuspended with 4μg of recombinant human homotypic-linked polyubiquitin chains Ub3-7 (K63 or K48, 4μg/600μl reaction volume, Boston Biochem, R&D) and 500μM ε-NAD in 1X ART buffer for 2 hrs with gentle end-over-end rotation at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μM ubiquitin aldehyde (Boston Biochem), 10 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μM Ub-VS (Boston Biochem) was added to the proteasomes binding resin and incubated for 2 h at 30 °C ...
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bioRxiv - Biochemistry 2021Quote: ... RPN2 (Boston Biochem AP-104, 1:1000), RPN13 (custom made13 ...
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bioRxiv - Molecular Biology 2019Quote: Purified xlCRL4CSA was neddylated in vitro using the NEDD8 Conjugation Initiation Kit (Boston Biochem) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... The DUBs used in this study are USP2 (Enzo, BML-UW9850-0100, 1 µM and Boston Biochem, E-504-0650, 1 µM), vOTU (1 µM) ...
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bioRxiv - Neuroscience 2019Quote: ... 1 µM His-Ubiquitin (Boston Biochem, Cat#U-530), 50 nM mouse E1 enzyme ...
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bioRxiv - Immunology 2019Quote: ... and 1 µg of mouse E2 (UBE2E3, Boston Biochem) in 40 µl of reaction buffer (Boston Biochem ...
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bioRxiv - Cell Biology 2023Quote: ... 225 nM E1-UBE-1 (Boston Biochem, E-305), 1.25 μM E2-UbcH5b (Boston Biochem ...
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bioRxiv - Cell Biology 2023Quote: The in vitro ubiquitination assay was performed using an ubiquitin kit (Boston Biochem, #K-230). His-tagged S5a protein (provided in the kit as positive control ...
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bioRxiv - Immunology 2021Quote: ... E2 (1 μM, 1.2 μl, UbcH5a, Boston Biochem, E2-616), E3 (0.5 μM ...
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bioRxiv - Biochemistry 2021Quote: ... 1.4 μM WW-HECT and 1 mg/ml ubiquitin (Boston Biochem) were incubated in reaction buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Microbiology 2024Quote: ... MgCl (1 mM) and 1x Ubiquitination buffer (Boston Biochem; Table S4) were added and the reaction was incubated at 30°C for 10 min ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of 10 mg/ml Ub solution (Boston Biochem; #U-100H), and 1μl radiolabeled in vitro translated protein of interest ...
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bioRxiv - Biochemistry 2021Quote: ... using 0.02 µg ubiquitin-activating enzyme 1 (His6-E1, Boston Biochem Inc.), 0.5 mM ATP and 10 mM MgCl2 in 50 mM Tris/HCl pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of 10 mg/ml Ubiquitin (Ub) solution (Boston Biochem; #U-100H) and 1 µl radiolabeled IVT product of interest ...
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bioRxiv - Biochemistry 2021Quote: ... USP8 was mixed with 1-μM ubiquitin-AMC (Boston Biochem or Life Sensors) and 10-mM DTT in TBS in 96-well medium-binding ...
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bioRxiv - Biochemistry 2020Quote: ... Assays of GST-RINGRNF213 activity were performed by using the E2Select Ubiquitin Conjugation Kit (Boston Biochem, Cat. No. K-982), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The bead-bound PfSkp1GFP and PfCullin-2/cDDHA immunoprecipitates were assessed for ubiquitination activity using a ubiquitin conjugation initiation kit according to the manufacturer’s instructions (Boston Biochem). Briefly ...
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bioRxiv - Immunology 2021Quote: ... Ubiquitination assays were performed at 37 °C for 90 minutes using loaded E1 (~ 80 ng, 3 μl, Boston Biochem kit, K-995), E2 (1 μM ...
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bioRxiv - Biochemistry 2021Quote: USP8 immunoprecipitates or His-tagged FRIP8 precipitates were incubated with 1-μM ubiquitin-VME (Boston Biochem) in TBS supplemented with 1-mM DTT at 37°C for 5 min or 30 min ...
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bioRxiv - Microbiology 2023Quote: ... purified proteasome (1 nM final concentration) was activated with PA28 (12nM final concentration; E380 Boston Biochem) followed by addition of Boc-LRR-AMC (50 µM final concentration ...
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bioRxiv - Biochemistry 2021Quote: ... We added 29 µM of the enzyme:peptide mixture to 1 mL of 30 µM Ub-AMC (7-amino-4- methylcoumarin) (Boston Biochem), for a final concentration of 200 nM DUBm ...