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Citations for Boston Biochem :
1 - 25 of 25 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... proTAME (APC/C inhibitor, I-440, Boston Biochem), Cytochalasin B (actin inhibitor ...
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bioRxiv - Plant Biology 2021Quote: ... 10 μg unlabeled ubiquitin (Boston Biochem), and 2 μg MBP-COP1 (previously incubated with 20 μM ZnCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg of Myc-ubiquitin (Boston Biochem), 1 μM ubiquitin aldehyde (Boston Biochem) ...
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bioRxiv - Cell Biology 2021Quote: ... energy regeneration solution (B-10, Boston Biochem) in assay buffer were carefully pipetted directly on the arrays ...
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bioRxiv - Biophysics 2024Quote: ... We added 10 μL of the enzyme:peptide mixture to 10 μL of 250 nM Ub-AMC (7-amino-4-methylcoumarin) (Boston Biochem). DUB activity was measured by monitoring the increase in AMC fluorescence in a plate reader ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μM HA-Ub (Boston Biochem, U-110), and 10 μM MgATP (Boston Biochem ...
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bioRxiv - Cell Biology 2019Quote: ... and 10 μM MgATP (Boston Biochem, B-20), in a final volume of 30 μl of E3 ligase buffer (Boston Biochem ...
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bioRxiv - Cell Biology 2019Quote: ... 200 nM BI2536 and 10 µM MG132 (Boston Biochem). Cycloheximide (CHX ...
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bioRxiv - Cell Biology 2022Quote: The reactions were run at 37°C for 90 min using 60 µM Ubiquitin (Boston Biochem) in the presence of 100 nM E1 (UBE1 ...
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bioRxiv - Genomics 2019Quote: ... For SUMOylation assays an antibody (A-603, Boston Biochem) directed against the SUMO1 substrate peptide (E-25K ...
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bioRxiv - Molecular Biology 2024Quote: ... and the rest was rotated overnight at 4°C with 50 µL commercial ubiquitin-conjugated TUBE beads (Boston Biochem). The beads were washed three times with 10 ml high salt TAP buffer (50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of 10 mg/ml Ub solution (Boston Biochem; #U-100H), and 1μl radiolabeled in vitro translated protein of interest ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of 10 mg/ml Ubiquitin (Ub) solution (Boston Biochem; #U-100H) and 1 µl radiolabeled IVT product of interest ...
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bioRxiv - Cell Biology 2019Quote: ... APC/C inhibition at anaphase onset on hTERT-RPE1 cells was achieved with a combination of both pro-TAME (Boston Biochem) and Apcin (Tocris Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... The antibodies used were as follows: UBE2C (Boston Biochem, Cat# A650) and beta-actin (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Ubiquitination assays were performed at 37 °C for 90 minutes using loaded E1 (~ 80 ng, 3 μl, Boston Biochem kit, K-995), E2 (1 μM ...
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bioRxiv - Biochemistry 2021Quote: ... were charged with 10 µg ubiquitin or N-terminally fluorescein-labelled Ub (FluoUb, Boston Biochem) using 0.02 µg ubiquitin-activating enzyme 1 (His6-E1 ...
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bioRxiv - Neuroscience 2021Quote: ... Sf 21 (baculovirus)-derived human USP30 protein) (10 nM; final assay concentration = 5 nM; Boston Biochem) was prepared in USP30 activity assay buffer (50 mM Tris base ...
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bioRxiv - Biochemistry 2022Quote: ... 0.16 μM myc-Cyclin D1-CDK4 complex and 10 mM MgATP solution (Boston Biochem B-20) in E3 ligase reaction buffer (Boston Biochem B-71) ...
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bioRxiv - Molecular Biology 2020Quote: ... translations were performed in the presence of 10 μM HA- or His-tagged Ubiquitin (Boston Biochem, USA). Denaturing immunoprecipitation of the ubiquitinated species was performed as previously described (Yanagitani et al. ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Commercial antibodies used for Western blotting analysis were as follow: anti-Ube2C (A-650, Boston Biochem), anti-ubiquitin (P4D1 ...
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bioRxiv - Cancer Biology 2022Quote: ... UBE2C protein levels were assessed by immunohistochemical (IHC) staining with UBE2C antibody (Boston Biochem, Cat# A650) or Ki-67 (D2H10 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 mM Na3VO4) at rt for 5-10 min before addition of master mix (42 pmol E1 (UBE1, Boston Biochem or Ubiquitin-Proteasome Biotechnologies) ...
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bioRxiv - Biochemistry 2023Quote: ... 25 ×106 cells per condition) and subjected to immunoprecipitation using 5 µg of ISG15 antibody (Boston Biochem #A-380) plus 25 μL of protein G Sepharose slurry (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... phosphor and protease inhibitor cocktails (25 x 106cells per condition) and subjected to immunoprecipitation using 5 μg ISG15 antibodies (Boston Biochem #A-380) plus 25 μL of protein G Sepharose slurry (Invitrogen ...