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Citations for Boston Biochem :
1 - 34 of 34 citations for Dengue Virus NS1 Protein Serotypes 1 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... We added 29 µM of the enzyme:peptide mixture to 1 mL of 30 µM Ub-AMC (7-amino-4- methylcoumarin) (Boston Biochem), for a final concentration of 200 nM DUBm ...
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bioRxiv - Synthetic Biology 2021Quote: ... 4 μM human UbcH5α/UBE2D1 (Boston Biochem), 3 μM uAb (or equivalent control protein) ...
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bioRxiv - Neuroscience 2024Quote: ... Non-phosphorylated Ub protein (Boston Biochem, U-100H), free non-phosphorylated ...
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bioRxiv - Biochemistry 2021Quote: ... Tetra-phospho-ubiquitin chains (pUb)4 were obtained from Boston Biochem.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Ubiquitin−7-amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15−7-amino-4-methylcourmarin (ISG15-AMC ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were incubated with 50 μl reaction mixture (Boston Biochem), 2 mM Mg-ATP (abcam) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human ISG15−7-amino-4-methylcourmarin (ISG15-AMC) was purchased from Boston Biochem. Lys48 linked tetra-Ub were obtained from Boston Biochem ...
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bioRxiv - Microbiology 2021Quote: ... with the following substitutions: USP7 assays contained 4 nM USP7 and 0.5 uM Ub-AMC (Boston Biochem); USP14 assays contained 1.7 uM USP14 ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant human BAP1-FL-WT protein was purchased from Boston Biochem (E-345-050). The Duet-His-ULD/AB protein complex was purified under native purification conditions using Cobalt beads (Talon) ...
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bioRxiv - Cancer Biology 2022Quote: ... UBE2C protein levels were assessed by immunohistochemical (IHC) staining with UBE2C antibody (Boston Biochem, Cat# A650) or Ki-67 (D2H10 ...
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bioRxiv - Neuroscience 2021Quote: ... Sf 21 (baculovirus)-derived human USP30 protein) (10 nM; final assay concentration = 5 nM; Boston Biochem) was prepared in USP30 activity assay buffer (50 mM Tris base ...
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bioRxiv - Microbiology 2022Quote: ... The reactions were initiated by the addition of 250 nM ubiquitin-7-amido-4-methylcoumarin (Ub-AMC, Boston Biochem) substrate to PfUBP-1 wt or catalytic inactive PfUBP-1 C3179A mutant ...
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bioRxiv - Molecular Biology 2024Quote: ... and the rest was rotated overnight at 4°C with 50 µL commercial ubiquitin-conjugated TUBE beads (Boston Biochem). The beads were washed three times with 10 ml high salt TAP buffer (50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Developmental Biology 2022Quote: ... through detection of the 7-amino-4-methylcoumarin (AMC) labeled fluorogenic peptide substrates Z-LLE-AMC (Boston Biochem #S-230) and LLVY-AMC (Chemicon #APT280) ...
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bioRxiv - Biophysics 2024Quote: ... We added 10 μL of the enzyme:peptide mixture to 10 μL of 250 nM Ub-AMC (7-amino-4-methylcoumarin) (Boston Biochem). DUB activity was measured by monitoring the increase in AMC fluorescence in a plate reader ...
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bioRxiv - Cell Biology 2021Quote: ... the bait GST or GST fusion protein was incubated with purified human 26S proteasome (E365; Boston Biochem, Cambridge, MA, USA) at 4 °C for 2 h in binding buffer (1× PBS ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μM ubiquitin aldehyde (Boston Biochem), 10 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μM Ub-VS (Boston Biochem) was added to the proteasomes binding resin and incubated for 2 h at 30 °C ...
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bioRxiv - Neuroscience 2024Quote: ... the supernatant of each single B cell well was screened for antigen specificity through direct ELISA for targeting full-length monomeric p-S65-Ub protein (Boston Biochem, U-102), free p-S65-Ub peptides 1 and 2 and BSA-conjugated p-S65-Ub peptides 1 and 2 (provided by 21st Century Biochemicals) ...
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bioRxiv - Biochemistry 2021Quote: ... RPN2 (Boston Biochem AP-104, 1:1000), RPN13 (custom made13 ...
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bioRxiv - Neuroscience 2020Quote: ... an equal amount of protein lysate (5 μg in 20 μl lysis buffer) was premixed with either 250 ng of proteasome inhibitor (epoxomicin, Boston Biochem, prepared in 50% dimethyl sulfoxide) or an equivalent volume of vehicle (50% dimethyl sulfoxide lacking the inhibitor) ...
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bioRxiv - Cell Biology 2024Quote: ... The DUBs used in this study are USP2 (Enzo, BML-UW9850-0100, 1 µM and Boston Biochem, E-504-0650, 1 µM), vOTU (1 µM) ...
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bioRxiv - Neuroscience 2019Quote: ... 1 µM His-Ubiquitin (Boston Biochem, Cat#U-530), 50 nM mouse E1 enzyme ...
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bioRxiv - Immunology 2019Quote: ... and 1 µg of mouse E2 (UBE2E3, Boston Biochem) in 40 µl of reaction buffer (Boston Biochem ...
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bioRxiv - Cell Biology 2023Quote: ... 225 nM E1-UBE-1 (Boston Biochem, E-305), 1.25 μM E2-UbcH5b (Boston Biochem ...
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bioRxiv - Immunology 2021Quote: ... E2 (1 μM, 1.2 μl, UbcH5a, Boston Biochem, E2-616), E3 (0.5 μM ...
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bioRxiv - Biochemistry 2021Quote: ... 1.4 μM WW-HECT and 1 mg/ml ubiquitin (Boston Biochem) were incubated in reaction buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Microbiology 2024Quote: ... MgCl (1 mM) and 1x Ubiquitination buffer (Boston Biochem; Table S4) were added and the reaction was incubated at 30°C for 10 min ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of 10 mg/ml Ub solution (Boston Biochem; #U-100H), and 1μl radiolabeled in vitro translated protein of interest ...
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bioRxiv - Biochemistry 2021Quote: ... using 0.02 µg ubiquitin-activating enzyme 1 (His6-E1, Boston Biochem Inc.), 0.5 mM ATP and 10 mM MgCl2 in 50 mM Tris/HCl pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of 10 mg/ml Ubiquitin (Ub) solution (Boston Biochem; #U-100H) and 1 µl radiolabeled IVT product of interest ...
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bioRxiv - Biochemistry 2021Quote: ... USP8 was mixed with 1-μM ubiquitin-AMC (Boston Biochem or Life Sensors) and 10-mM DTT in TBS in 96-well medium-binding ...
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bioRxiv - Biochemistry 2021Quote: USP8 immunoprecipitates or His-tagged FRIP8 precipitates were incubated with 1-μM ubiquitin-VME (Boston Biochem) in TBS supplemented with 1-mM DTT at 37°C for 5 min or 30 min ...
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bioRxiv - Microbiology 2023Quote: ... purified proteasome (1 nM final concentration) was activated with PA28 (12nM final concentration; E380 Boston Biochem) followed by addition of Boc-LRR-AMC (50 µM final concentration ...