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Citations for Boston Biochem :
1 - 22 of 22 citations for Cell Division Cycle 2 Like Protein Kinase 6 CDC2L6 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Boc-L-R-R-AMC (trypsin-like activity) and Z-L-L-E-AMC (caspase-like activity) (Boston Biochem). The proteasome samples were incubated with 50 μM AMC-peptide in the respective purification buffers (standard or the exogenous nucleotide depleted buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... UBE2C protein levels were assessed by immunohistochemical (IHC) staining with UBE2C antibody (Boston Biochem, Cat# A650) or Ki-67 (D2H10 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were synthesized by solid-phase synthesis through iterative cycles of coupling and deprotection using the previously optimized procedure.27Ub-AMC and ISG15-AMC were purchased from Boston Biochem (catalog # U-550-050 and UL-553-050 respectively).
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bioRxiv - Immunology 2021Quote: ... E3 (0.5 μM, 6 μl, His-cIAP1, Boston Biochem, E3-280) and 15 μl of purified FLAG-IL-1β in Ubiquitin assay buffer containing 2 mM ATP (total volume of 30 μl) ...
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bioRxiv - Neuroscience 2024Quote: ... the supernatant of each single B cell well was screened for antigen specificity through direct ELISA for targeting full-length monomeric p-S65-Ub protein (Boston Biochem, U-102), free p-S65-Ub peptides 1 and 2 and BSA-conjugated p-S65-Ub peptides 1 and 2 (provided by 21st Century Biochemicals) ...
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bioRxiv - Biochemistry 2023Quote: ... 25 ×106 cells per condition) and subjected to immunoprecipitation using 5 µg of ISG15 antibody (Boston Biochem #A-380) plus 25 μL of protein G Sepharose slurry (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Non-phosphorylated Ub protein (Boston Biochem, U-100H), free non-phosphorylated ...
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bioRxiv - Biophysics 2021Quote: ... E2 was charged at 37°C for 30 min (for yeast: reaction contained mg/ml Uba1, 2 mg/ml Ubc4, 2 mg/ml methylated ubiquitin from Boston Biochem #Y-501 ...
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bioRxiv - Biochemistry 2019Quote: ... 2 mg/ml Methylated ubiquitin (Boston Biochem #U-501) and 1 mM ATP at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were incubated with 50 μl reaction mixture (Boston Biochem), 2 mM Mg-ATP (abcam) ...
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bioRxiv - Genomics 2019Quote: ... For SUMOylation assays an antibody (A-603, Boston Biochem) directed against the SUMO1 substrate peptide (E-25K ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant human BAP1-FL-WT protein was purchased from Boston Biochem (E-345-050). The Duet-His-ULD/AB protein complex was purified under native purification conditions using Cobalt beads (Talon) ...
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bioRxiv - Neuroscience 2021Quote: ... Sf 21 (baculovirus)-derived human USP30 protein) (10 nM; final assay concentration = 5 nM; Boston Biochem) was prepared in USP30 activity assay buffer (50 mM Tris base ...
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bioRxiv - Cancer Biology 2022Quote: ... The antibodies used were as follows: UBE2C (Boston Biochem, Cat# A650) and beta-actin (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... the bait GST or GST fusion protein was incubated with purified human 26S proteasome (E365; Boston Biochem, Cambridge, MA, USA) at 4 °C for 2 h in binding buffer (1× PBS ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Commercial antibodies used for Western blotting analysis were as follow: anti-Ube2C (A-650, Boston Biochem), anti-ubiquitin (P4D1 ...
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bioRxiv - Neuroscience 2020Quote: ... an equal amount of protein lysate (5 μg in 20 μl lysis buffer) was premixed with either 250 ng of proteasome inhibitor (epoxomicin, Boston Biochem, prepared in 50% dimethyl sulfoxide) or an equivalent volume of vehicle (50% dimethyl sulfoxide lacking the inhibitor) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with 50 μM Apcin (I-444, Boston Biochem) and 20 μM proTAME (I-440 ...
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bioRxiv - Cancer Biology 2020Quote: ... phosphor and protease inhibitor cocktails (25 x 106cells per condition) and subjected to immunoprecipitation using 5 μg ISG15 antibodies (Boston Biochem #A-380) plus 25 μL of protein G Sepharose slurry (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... The treated cells were incubated with the 5 µM proteasome activity probe Me4BodipyFl-Ahx3Leu3VS (Boston Biochem) for 2 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed with cold PBS and lysed in 300 µL 1x Ubiquitination buffer (Boston Biochem; Table S4) containing 1% Triton X-100 ...
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bioRxiv - Cell Biology 2019Quote: ... APC/C inhibition at anaphase onset on hTERT-RPE1 cells was achieved with a combination of both pro-TAME (Boston Biochem) and Apcin (Tocris Bioscience ...