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Citations for Boston Biochem :
1 - 28 of 28 citations for 8 4 dimethylaminophenyl diazenyl N N dimethyl 10 phenylphenazin 10 ium 2 amine chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... were charged with 10 µg ubiquitin or N-terminally fluorescein-labelled Ub (FluoUb, Boston Biochem) using 0.02 µg ubiquitin-activating enzyme 1 (His6-E1 ...
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bioRxiv - Biophysics 2024Quote: ... We added 10 μL of the enzyme:peptide mixture to 10 μL of 250 nM Ub-AMC (7-amino-4-methylcoumarin) (Boston Biochem). DUB activity was measured by monitoring the increase in AMC fluorescence in a plate reader ...
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bioRxiv - Biochemistry 2021Quote: ... 20 µL of two-fold dilution series of ligand and 0.2 µM N-terminally FITC-labeled Ub (Boston Biochem) prepared in buffer were incubated in wells for 1 hour at 4⁰C ...
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bioRxiv - Plant Biology 2021Quote: ... 10 μg unlabeled ubiquitin (Boston Biochem), and 2 μg MBP-COP1 (previously incubated with 20 μM ZnCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg of Myc-ubiquitin (Boston Biochem), 1 μM ubiquitin aldehyde (Boston Biochem) ...
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bioRxiv - Cell Biology 2021Quote: ... energy regeneration solution (B-10, Boston Biochem) in assay buffer were carefully pipetted directly on the arrays ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μM HA-Ub (Boston Biochem, U-110), and 10 μM MgATP (Boston Biochem ...
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bioRxiv - Cell Biology 2019Quote: ... and 10 μM MgATP (Boston Biochem, B-20), in a final volume of 30 μl of E3 ligase buffer (Boston Biochem ...
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bioRxiv - Cell Biology 2019Quote: ... 200 nM BI2536 and 10 µM MG132 (Boston Biochem). Cycloheximide (CHX ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of 10 mg/ml Ub solution (Boston Biochem; #U-100H), and 1μl radiolabeled in vitro translated protein of interest ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of 10 mg/ml Ubiquitin (Ub) solution (Boston Biochem; #U-100H) and 1 µl radiolabeled IVT product of interest ...
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bioRxiv - Neuroscience 2021Quote: ... Sf 21 (baculovirus)-derived human USP30 protein) (10 nM; final assay concentration = 5 nM; Boston Biochem) was prepared in USP30 activity assay buffer (50 mM Tris base ...
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bioRxiv - Biochemistry 2022Quote: ... 0.16 μM myc-Cyclin D1-CDK4 complex and 10 mM MgATP solution (Boston Biochem B-20) in E3 ligase reaction buffer (Boston Biochem B-71) ...
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bioRxiv - Molecular Biology 2020Quote: ... translations were performed in the presence of 10 μM HA- or His-tagged Ubiquitin (Boston Biochem, USA). Denaturing immunoprecipitation of the ubiquitinated species was performed as previously described (Yanagitani et al. ...
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bioRxiv - Neuroscience 2020Quote: ... an equal amount of protein lysate (5 μg in 20 μl lysis buffer) was premixed with either 250 ng of proteasome inhibitor (epoxomicin, Boston Biochem, prepared in 50% dimethyl sulfoxide) or an equivalent volume of vehicle (50% dimethyl sulfoxide lacking the inhibitor) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 mM Na3VO4) at rt for 5-10 min before addition of master mix (42 pmol E1 (UBE1, Boston Biochem or Ubiquitin-Proteasome Biotechnologies) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 4 μM human UbcH5α/UBE2D1 (Boston Biochem), 3 μM uAb (or equivalent control protein) ...
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bioRxiv - Biochemistry 2021Quote: ... Tetra-phospho-ubiquitin chains (pUb)4 were obtained from Boston Biochem.
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bioRxiv - Biochemistry 2023Quote: DDB1 (3.3 uL, 8 μM) was mixed with CUL4A/NEDD8/RBX1 (0.2 uL, 9 μM, Boston Biochem. Inc., E3-441-025), UBE1 (0.2 μL ...
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bioRxiv - Biophysics 2021Quote: ... E2 was charged at 37°C for 30 min (for yeast: reaction contained mg/ml Uba1, 2 mg/ml Ubc4, 2 mg/ml methylated ubiquitin from Boston Biochem #Y-501 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Ubiquitin−7-amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15−7-amino-4-methylcourmarin (ISG15-AMC ...
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bioRxiv - Biochemistry 2019Quote: ... 2 mg/ml Methylated ubiquitin (Boston Biochem #U-501) and 1 mM ATP at 37°C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human ISG15−7-amino-4-methylcourmarin (ISG15-AMC) was purchased from Boston Biochem. Lys48 linked tetra-Ub were obtained from Boston Biochem ...
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bioRxiv - Microbiology 2021Quote: ... with the following substitutions: USP7 assays contained 4 nM USP7 and 0.5 uM Ub-AMC (Boston Biochem); USP14 assays contained 1.7 uM USP14 ...
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bioRxiv - Microbiology 2022Quote: ... The reactions were initiated by the addition of 250 nM ubiquitin-7-amido-4-methylcoumarin (Ub-AMC, Boston Biochem) substrate to PfUBP-1 wt or catalytic inactive PfUBP-1 C3179A mutant ...
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bioRxiv - Molecular Biology 2024Quote: ... and the rest was rotated overnight at 4°C with 50 µL commercial ubiquitin-conjugated TUBE beads (Boston Biochem). The beads were washed three times with 10 ml high salt TAP buffer (50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Developmental Biology 2022Quote: ... through detection of the 7-amino-4-methylcoumarin (AMC) labeled fluorogenic peptide substrates Z-LLE-AMC (Boston Biochem #S-230) and LLVY-AMC (Chemicon #APT280) ...
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bioRxiv - Biochemistry 2021Quote: ... We added 29 µM of the enzyme:peptide mixture to 1 mL of 30 µM Ub-AMC (7-amino-4- methylcoumarin) (Boston Biochem), for a final concentration of 200 nM DUBm ...