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Citations for Boston Biochem :
1 - 16 of 16 citations for 6 methyl 2 thioxo 2 3 dihydroquinazolin 4 1H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... E2 was charged at 37°C for 30 min (for yeast: reaction contained mg/ml Uba1, 2 mg/ml Ubc4, 2 mg/ml methylated ubiquitin from Boston Biochem #Y-501 ...
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bioRxiv - Biochemistry 2019Quote: ... 2 mg/ml Methylated ubiquitin (Boston Biochem #U-501) and 1 mM ATP at 37°C for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... E3 (0.5 μM, 6 μl, His-cIAP1, Boston Biochem, E3-280) and 15 μl of purified FLAG-IL-1β in Ubiquitin assay buffer containing 2 mM ATP (total volume of 30 μl) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 4 μM human UbcH5α/UBE2D1 (Boston Biochem), 3 μM uAb (or equivalent control protein) ...
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bioRxiv - Cell Biology 2019Quote: ... ii) mock or 0.4 mg/ml of one of the following unlabled Ubiqutin variants: WT (Boston Biochem; #U100-H), Lys11 to Arg mutant (Boston Biochem ...
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bioRxiv - Biochemistry 2021Quote: ... Tetra-phospho-ubiquitin chains (pUb)4 were obtained from Boston Biochem.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Ubiquitin−7-amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15−7-amino-4-methylcourmarin (ISG15-AMC ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence of hydrolyzed K63-linked diUb (TAMRA/QXL position 3 labeled, Boston Biochem #UF-330) was measured on a BioTek synergy LX plate reader equipped with a red filter cube assembly (ex ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human ISG15−7-amino-4-methylcourmarin (ISG15-AMC) was purchased from Boston Biochem. Lys48 linked tetra-Ub were obtained from Boston Biochem ...
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bioRxiv - Microbiology 2021Quote: ... with the following substitutions: USP7 assays contained 4 nM USP7 and 0.5 uM Ub-AMC (Boston Biochem); USP14 assays contained 1.7 uM USP14 ...
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bioRxiv - Microbiology 2022Quote: ... The reactions were initiated by the addition of 250 nM ubiquitin-7-amido-4-methylcoumarin (Ub-AMC, Boston Biochem) substrate to PfUBP-1 wt or catalytic inactive PfUBP-1 C3179A mutant ...
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bioRxiv - Molecular Biology 2024Quote: ... and the rest was rotated overnight at 4°C with 50 µL commercial ubiquitin-conjugated TUBE beads (Boston Biochem). The beads were washed three times with 10 ml high salt TAP buffer (50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Immunology 2021Quote: ... Ubiquitination assays were performed at 37 °C for 90 minutes using loaded E1 (~ 80 ng, 3 μl, Boston Biochem kit, K-995), E2 (1 μM ...
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bioRxiv - Developmental Biology 2022Quote: ... through detection of the 7-amino-4-methylcoumarin (AMC) labeled fluorogenic peptide substrates Z-LLE-AMC (Boston Biochem #S-230) and LLVY-AMC (Chemicon #APT280) ...
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bioRxiv - Biochemistry 2021Quote: ... We added 29 µM of the enzyme:peptide mixture to 1 mL of 30 µM Ub-AMC (7-amino-4- methylcoumarin) (Boston Biochem), for a final concentration of 200 nM DUBm ...
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bioRxiv - Biophysics 2024Quote: ... We added 10 μL of the enzyme:peptide mixture to 10 μL of 250 nM Ub-AMC (7-amino-4-methylcoumarin) (Boston Biochem). DUB activity was measured by monitoring the increase in AMC fluorescence in a plate reader ...