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Citations for Boston Biochem :
1 - 40 of 40 citations for 5 OXO 5 6 7 8 TETRAHYDRO NAPHTHALENE 1 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... pH 7.5 and 0.5 mM ethylenediaminetetraacetic acid) containing a chymotryptic substrate (80 μM Suc-Leu-Leu-Val-Tyr -AMC; Boston Biochem). Reactions were incubated at 25°C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... 5 µM Ub (U-100H, Boston Biochem) and 5 µM UbcH7 (E2-640 ...
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bioRxiv - Biochemistry 2020Quote: ... or 5 µM tetra-ubiquitin (Boston Biochem) at RT ...
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bioRxiv - Microbiology 2020Quote: ... 5 µM Ub (U-100H, Boston Biochem), 5 µM UbcH7 (E2-640 ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mg His-ubiquitin (BB-U-530, Boston Biochem), 2 mg purified MBP-KEG fusion protein (as E3 enzyme) ...
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bioRxiv - Biochemistry 2023Quote: ... UBE1 (0.2 μL, 5 μM, Boston Biochem. Inc., E-305-025), UBE2D1 (2.6 μL ...
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bioRxiv - Biochemistry 2023Quote: ... UBE1 (0.4 μL, 5 μM, Boston Biochem. Inc., E-305-025), UBE2D1 (5.2 μL ...
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bioRxiv - Microbiology 2023Quote: ... reaction mixtures (12.5 μl) containing 5 μg of recombinant human Ub (Boston Biochem), 80 nM recombinant human E1 (Boston Biochem) ...
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bioRxiv - Molecular Biology 2023Quote: ... in the presence of 5 nM of 20S proteasome (Boston Biochem, E-360), at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... The treated cells were incubated with the 5 µM proteasome activity probe Me4BodipyFl-Ahx3Leu3VS (Boston Biochem) for 2 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Sf 21 (baculovirus)-derived human USP30 protein) (10 nM; final assay concentration = 5 nM; Boston Biochem) was prepared in USP30 activity assay buffer (50 mM Tris base ...
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bioRxiv - Biochemistry 2021Quote: ... We added 29 µM of the enzyme:peptide mixture to 1 mL of 30 µM Ub-AMC (7-amino-4- methylcoumarin) (Boston Biochem), for a final concentration of 200 nM DUBm ...
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bioRxiv - Biochemistry 2023Quote: ... 25 ×106 cells per condition) and subjected to immunoprecipitation using 5 µg of ISG15 antibody (Boston Biochem #A-380) plus 25 μL of protein G Sepharose slurry (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 mM Na3VO4) at rt for 5-10 min before addition of master mix (42 pmol E1 (UBE1, Boston Biochem or Ubiquitin-Proteasome Biotechnologies) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Ubiquitin−7-amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15−7-amino-4-methylcourmarin (ISG15-AMC ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human ISG15−7-amino-4-methylcourmarin (ISG15-AMC) was purchased from Boston Biochem. Lys48 linked tetra-Ub were obtained from Boston Biochem ...
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bioRxiv - Cancer Biology 2020Quote: ... phosphor and protease inhibitor cocktails (25 x 106cells per condition) and subjected to immunoprecipitation using 5 μg ISG15 antibodies (Boston Biochem #A-380) plus 25 μL of protein G Sepharose slurry (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... an equal amount of protein lysate (5 μg in 20 μl lysis buffer) was premixed with either 250 ng of proteasome inhibitor (epoxomicin, Boston Biochem, prepared in 50% dimethyl sulfoxide) or an equivalent volume of vehicle (50% dimethyl sulfoxide lacking the inhibitor) ...
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bioRxiv - Immunology 2021Quote: ... E3 (0.5 μM, 6 μl, His-cIAP1, Boston Biochem, E3-280) and 15 μl of purified FLAG-IL-1β in Ubiquitin assay buffer containing 2 mM ATP (total volume of 30 μl) ...
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bioRxiv - Microbiology 2022Quote: ... The reactions were initiated by the addition of 250 nM ubiquitin-7-amido-4-methylcoumarin (Ub-AMC, Boston Biochem) substrate to PfUBP-1 wt or catalytic inactive PfUBP-1 C3179A mutant ...
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bioRxiv - Developmental Biology 2022Quote: ... through detection of the 7-amino-4-methylcoumarin (AMC) labeled fluorogenic peptide substrates Z-LLE-AMC (Boston Biochem #S-230) and LLVY-AMC (Chemicon #APT280) ...
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bioRxiv - Biophysics 2024Quote: ... We added 10 μL of the enzyme:peptide mixture to 10 μL of 250 nM Ub-AMC (7-amino-4-methylcoumarin) (Boston Biochem). DUB activity was measured by monitoring the increase in AMC fluorescence in a plate reader ...
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bioRxiv - Microbiology 2023Quote: ... each 25 μl pelleted resin was resuspended with 4μg of recombinant human homotypic-linked polyubiquitin chains Ub3-7 (K63 or K48, 4μg/600μl reaction volume, Boston Biochem, R&D) and 500μM ε-NAD in 1X ART buffer for 2 hrs with gentle end-over-end rotation at 37°C ...
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bioRxiv - Biochemistry 2023Quote: DDB1 (3.3 uL, 8 μM) was mixed with CUL4A/NEDD8/RBX1 (0.2 uL, 9 μM, Boston Biochem. Inc., E3-441-025), UBE1 (0.2 μL ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μM ubiquitin aldehyde (Boston Biochem), 10 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μM Ub-VS (Boston Biochem) was added to the proteasomes binding resin and incubated for 2 h at 30 °C ...
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bioRxiv - Biochemistry 2021Quote: ... RPN2 (Boston Biochem AP-104, 1:1000), RPN13 (custom made13 ...
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bioRxiv - Cell Biology 2024Quote: ... The DUBs used in this study are USP2 (Enzo, BML-UW9850-0100, 1 µM and Boston Biochem, E-504-0650, 1 µM), vOTU (1 µM) ...
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bioRxiv - Neuroscience 2019Quote: ... 1 µM His-Ubiquitin (Boston Biochem, Cat#U-530), 50 nM mouse E1 enzyme ...
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bioRxiv - Immunology 2019Quote: ... and 1 µg of mouse E2 (UBE2E3, Boston Biochem) in 40 µl of reaction buffer (Boston Biochem ...
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bioRxiv - Cell Biology 2023Quote: ... 225 nM E1-UBE-1 (Boston Biochem, E-305), 1.25 μM E2-UbcH5b (Boston Biochem ...
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bioRxiv - Immunology 2021Quote: ... E2 (1 μM, 1.2 μl, UbcH5a, Boston Biochem, E2-616), E3 (0.5 μM ...
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bioRxiv - Biochemistry 2021Quote: ... 1.4 μM WW-HECT and 1 mg/ml ubiquitin (Boston Biochem) were incubated in reaction buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Microbiology 2024Quote: ... MgCl (1 mM) and 1x Ubiquitination buffer (Boston Biochem; Table S4) were added and the reaction was incubated at 30°C for 10 min ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of 10 mg/ml Ub solution (Boston Biochem; #U-100H), and 1μl radiolabeled in vitro translated protein of interest ...
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bioRxiv - Biochemistry 2021Quote: ... using 0.02 µg ubiquitin-activating enzyme 1 (His6-E1, Boston Biochem Inc.), 0.5 mM ATP and 10 mM MgCl2 in 50 mM Tris/HCl pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of 10 mg/ml Ubiquitin (Ub) solution (Boston Biochem; #U-100H) and 1 µl radiolabeled IVT product of interest ...
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bioRxiv - Biochemistry 2021Quote: ... USP8 was mixed with 1-μM ubiquitin-AMC (Boston Biochem or Life Sensors) and 10-mM DTT in TBS in 96-well medium-binding ...
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bioRxiv - Biochemistry 2021Quote: USP8 immunoprecipitates or His-tagged FRIP8 precipitates were incubated with 1-μM ubiquitin-VME (Boston Biochem) in TBS supplemented with 1-mM DTT at 37°C for 5 min or 30 min ...
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bioRxiv - Microbiology 2023Quote: ... purified proteasome (1 nM final concentration) was activated with PA28 (12nM final concentration; E380 Boston Biochem) followed by addition of Boc-LRR-AMC (50 µM final concentration ...