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Citations for Boston Biochem :
1 - 32 of 32 citations for 26S Proteasome Non ATPase Regulatory Subunit 10 PSMD10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the bait GST or GST fusion protein was incubated with purified human 26S proteasome (E365; Boston Biochem, Cambridge, MA, USA) at 4 °C for 2 h in binding buffer (1× PBS ...
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bioRxiv - Microbiology 2022Quote: The proteasome inhibitor MG132 (Boston Biochem) was used in this study ...
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bioRxiv - Biochemistry 2021Quote: ... human proteasomes were treated with ubiquitin-vinyl-sulfone (UbVS, Boston Biochem). UbVS was added to the resin at 1-1.5 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was inhibited via addition of 200 μM MG262 (Boston Biochem). Egg extracts were supplemented with recombinant proteins as detailed below and incubated for 10 min at RT before addition of plasmid DNA unless otherwise stated ...
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bioRxiv - Molecular Biology 2019Quote: ... 26 µM Ubiquitin (Boston Biochem U-530), 0.3 µM UBE1 (Boston Biochem E-304-050) ...
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bioRxiv - Molecular Biology 2023Quote: ... in the presence of 5 nM of 20S proteasome (Boston Biochem, E-360), at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... reaction mixture was supplemented with proteasome inhibitor MG132 (20 μM; Boston Biochem; #I-130). Reaction mixtures were incubated at 28°C ...
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bioRxiv - Neuroscience 2021Quote: ... The treated cells were incubated with the 5 µM proteasome activity probe Me4BodipyFl-Ahx3Leu3VS (Boston Biochem) for 2 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... purified proteasome (1 nM final concentration) was activated with PA28 (12nM final concentration; E380 Boston Biochem) followed by addition of Boc-LRR-AMC (50 µM final concentration ...
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bioRxiv - Neuroscience 2024Quote: ... non-phosphorylated PRKN (Boston Biochem, E3-160) and p-S65-Ub monomer (Boston Biochem ...
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bioRxiv - Neuroscience 2024Quote: ... Non-phosphorylated Ub protein (Boston Biochem, U-100H), free non-phosphorylated ...
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bioRxiv - Neuroscience 2020Quote: ... an equal amount of protein lysate (5 μg in 20 μl lysis buffer) was premixed with either 250 ng of proteasome inhibitor (epoxomicin, Boston Biochem, prepared in 50% dimethyl sulfoxide) or an equivalent volume of vehicle (50% dimethyl sulfoxide lacking the inhibitor) ...
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bioRxiv - Plant Biology 2021Quote: ... 10 μg unlabeled ubiquitin (Boston Biochem), and 2 μg MBP-COP1 (previously incubated with 20 μM ZnCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg of Myc-ubiquitin (Boston Biochem), 1 μM ubiquitin aldehyde (Boston Biochem) ...
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bioRxiv - Cell Biology 2021Quote: ... energy regeneration solution (B-10, Boston Biochem) in assay buffer were carefully pipetted directly on the arrays ...
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bioRxiv - Biophysics 2024Quote: ... We added 10 μL of the enzyme:peptide mixture to 10 μL of 250 nM Ub-AMC (7-amino-4-methylcoumarin) (Boston Biochem). DUB activity was measured by monitoring the increase in AMC fluorescence in a plate reader ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μM HA-Ub (Boston Biochem, U-110), and 10 μM MgATP (Boston Biochem ...
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bioRxiv - Cell Biology 2019Quote: ... and 10 μM MgATP (Boston Biochem, B-20), in a final volume of 30 μl of E3 ligase buffer (Boston Biochem ...
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bioRxiv - Cell Biology 2019Quote: ... 200 nM BI2536 and 10 µM MG132 (Boston Biochem). Cycloheximide (CHX ...
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bioRxiv - Genomics 2019Quote: ... For SUMOylation assays an antibody (A-603, Boston Biochem) directed against the SUMO1 substrate peptide (E-25K ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of 10 mg/ml Ub solution (Boston Biochem; #U-100H), and 1μl radiolabeled in vitro translated protein of interest ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of 10 mg/ml Ubiquitin (Ub) solution (Boston Biochem; #U-100H) and 1 µl radiolabeled IVT product of interest ...
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bioRxiv - Cancer Biology 2022Quote: ... The antibodies used were as follows: UBE2C (Boston Biochem, Cat# A650) and beta-actin (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... were charged with 10 µg ubiquitin or N-terminally fluorescein-labelled Ub (FluoUb, Boston Biochem) using 0.02 µg ubiquitin-activating enzyme 1 (His6-E1 ...
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bioRxiv - Neuroscience 2021Quote: ... Sf 21 (baculovirus)-derived human USP30 protein) (10 nM; final assay concentration = 5 nM; Boston Biochem) was prepared in USP30 activity assay buffer (50 mM Tris base ...
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bioRxiv - Biochemistry 2022Quote: ... 0.16 μM myc-Cyclin D1-CDK4 complex and 10 mM MgATP solution (Boston Biochem B-20) in E3 ligase reaction buffer (Boston Biochem B-71) ...
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bioRxiv - Molecular Biology 2020Quote: ... translations were performed in the presence of 10 μM HA- or His-tagged Ubiquitin (Boston Biochem, USA). Denaturing immunoprecipitation of the ubiquitinated species was performed as previously described (Yanagitani et al. ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Commercial antibodies used for Western blotting analysis were as follow: anti-Ube2C (A-650, Boston Biochem), anti-ubiquitin (P4D1 ...
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bioRxiv - Cancer Biology 2022Quote: ... UBE2C protein levels were assessed by immunohistochemical (IHC) staining with UBE2C antibody (Boston Biochem, Cat# A650) or Ki-67 (D2H10 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 mM Na3VO4) at rt for 5-10 min before addition of master mix (42 pmol E1 (UBE1, Boston Biochem or Ubiquitin-Proteasome Biotechnologies) ...
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bioRxiv - Biochemistry 2023Quote: ... 25 ×106 cells per condition) and subjected to immunoprecipitation using 5 µg of ISG15 antibody (Boston Biochem #A-380) plus 25 μL of protein G Sepharose slurry (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... phosphor and protease inhibitor cocktails (25 x 106cells per condition) and subjected to immunoprecipitation using 5 μg ISG15 antibodies (Boston Biochem #A-380) plus 25 μL of protein G Sepharose slurry (Invitrogen ...