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Citations for New England Biolabs :
1 - 44 of 44 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The slides were then equilibrated in 1X Neutral Electrophoresis Buffer (NEB) buffer (1 M Tris base ...
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bioRxiv - Microbiology 2022Quote: ... After electrophoresis and gel extraction by Monarch DNA Gel Extraction Kit (NEB), the PCR product was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Biophysics 2020Quote: For the gel electrophoresis we added Gel Loading Dye Purple (6x) (NEB) to pBR322 DNA ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and gel electrophoresis with λ DNA-HindIII Digest marker (New England BioLabs). Samples were then sent to Novogene Corporation (Beijing ...
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bioRxiv - Cancer Biology 2023Quote: ... All gel electrophoresis was completed using a 1517bp-100bp ladder (BioLabs, N3231L). To analyze protein cellular protein ...
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bioRxiv - Microbiology 2022Quote: ... and resolved by electrophoresis in TBE buffer with low range ssRNA ladder (NEB). Gels were post-stained with SYBR gold for 1 hr at room temperature and imaged using BioRad Gel Doc EZ Imager.
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bioRxiv - Microbiology 2022Quote: ... After electrophoresis and gel extraction by Monarch DNA Gel Extraction Kit (T1020S; NEB), the PCR product was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (E2040S ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were then isolated via gel electrophoresis and assembled using HiFi assembly mix (NEB #E2621L). Plasmids were verified by DNA sequencing and stored at -20°C until use in NanoBRET assays.
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bioRxiv - Cancer Biology 2023Quote: ... the DNA fragments were purified from gel electrophoresis using the Monarch DNA gel extraction kit (NEB), fragments and destination plasmid were digested using the respective restriction enzymes (NEB ...
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bioRxiv - Systems Biology 2022Quote: ... Before electrophoresis, extracted plasmids were mixed with Purple Gel Loading Dye (6X, no SDS) (New England Biolabs) and loaded into wells in the agarose gel ...
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bioRxiv - Genetics 2021Quote: ... the fragments were analyzed by gel electrophoresis and purified using a Monarch DNA Gel Extraction Kit (NEB). All strains had the expected variants in the sequenced fragment ...
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bioRxiv - Genetics 2022Quote: ... PCR-products were gel purified after agarose gel electrophoresis with Monarch DNA Gel Extraction Kit (New England Biolabs) and submitted for sequencing ...
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bioRxiv - Genetics 2020Quote: ... Fragments for assembly were purified using agarose electrophoresis and gel extraction (Monarch DNA Gel Extraction Kit, NEB T1020L). For assembly ...
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bioRxiv - Genetics 2023Quote: ... PCR products were purified after agarose gel electrophoresis using the Monarch DNA Gel Extraction Kit (New England Biolabs) and submitted for Sanger sequencing to Eurofins Genomics ...
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bioRxiv - Biochemistry 2022Quote: ... Cleavage fragments were resolved by 0.8% agarose gel electrophoresis with 1 μL of 1 kb Plus DNA Ladder (NEB) and visualized by ethidium bromide staining ...
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bioRxiv - Developmental Biology 2019Quote: ... Genotyping was performed by agarose gel electrophoresis-mediated detection following PCR reaction by Taq polymerase (M0273, New England Biolabs). qPCR was performed using GoTaq® qPCR Master Mix (A6001 ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were separated by electrophoresis on 1.5% agarose gels stained with Green Gene Safe DNA Dye (Southern BioLabs). PCR products were purified using Zymo’s DNA Clean and Concentrator kit and sequenced with their respective primer sets to confirm correct insertion of the fluorescent tag.
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bioRxiv - Synthetic Biology 2023Quote: ... pH 8.0) agarose gel electrophoresis migration of each sample mixed with 6X loading buffer (Gel Loading Dye Purple NEB). DNA degradation was appreciated after ethidium bromide coloration (2µg/mL final concentration ...
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bioRxiv - Biochemistry 2021Quote: ... RNA integrity was assessed by performing 1% TBE agarose gel electrophoresis with samples that had been boiled for 95°C for 5 min in RNA loading dye (New England Biolabs). Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR products were resolved by agarose gel electrophoresis and purified using the Monarch Gel Extraction kit (New England Biolabs). Purified PCR products were Sanger sequenced by Genewiz using the BPNT1_F_Seq primer ...
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bioRxiv - Microbiology 2019Quote: ... The integrity of extracted DNA was confirmed by agarose gel electrophoresis by loading equal amount of extracted DNA on agarose gel along with גHindIII digest marker (NEB) 27 ...
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bioRxiv - Microbiology 2019Quote: ... DNA fragments were purified by agarose gel electrophoresis and assembled using Gibson Assembly mix (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... PCR product of the expected size was purified from the agarose gel after electrophoresis using the Monarch DNA Gel Extraction Kit (NEB), and cloned into the pDONR207 vector (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Products were identified with gel electrophoresis using a molecular weight marker (Low molecular weight marker, New England Biolabs, Frankfurt, Germany). As a positive control ...
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bioRxiv - Microbiology 2020Quote: ... An aliquot of each PCR reactions was checked by gel electrophoresis for single amplicons before purifying the remaining reaction using Monarch DNA Gel Extraction Kit (NEB), per manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were verified by agarose gel electrophoresis and purified using the Monarch PCR and DNA Cleanup Kit (New England Biolabs). In vitro transcription was performed using the T7 RiboMAX Express Large Scale RNA Production System (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... The products were analyzed by electrophoresis in a non-denaturing 12% polyacrylamide gel against a 5’-labeled Low Molecular Weight DNA Ladder (New England Biolabs) and quantitated with a phosphorimager (Typhoon FLA 9500 ...
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bioRxiv - Molecular Biology 2022Quote: ... and analyzed by electrophoresis in a non-denaturing 12% polyacrylamide gel with 5’-labeled Low Molecular Weight DNA Ladder (New England Biolabs) run in a parallel lane ...
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bioRxiv - Immunology 2023Quote: ... The pure genomic DNA was separated from any extrachromosomal vaccine DNA by 2D gel electrophoresis and further digested by the AscI restriction Enzyme (R0558S, NEB) since it cuts only DNA propagated in E ...
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bioRxiv - Biochemistry 2022Quote: ... The purity and approximate size of the cloned fragment were assessed by agarose gel electrophoresis after endonuclease digestion simultaneously with BamHI and XbaI (New England Biolabs, in NEBuffer 3.1 at 37° for 1 hour using 1 U of enzyme per 1 μg DNA) ...
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bioRxiv - Plant Biology 2024Quote: ... Amplification specificity was assessed by agarose gel electrophoresis and amplicons were subsequently purified with the Monarch PCR & DNA Clean Up Kit (NEB). Resulting DNA concentrations were spectrophotometrically measured by NanoDrop ...
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bioRxiv - Cell Biology 2020Quote: ... The presence of the PCR products was confirmed by gel electrophoresis and the products were then purified by Monarch® PCR & DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Microbiology 2019Quote: ... DNA fragments with the size of 350-700 bp were separated on an agarose gel during electrophoresis and purified for input into the NEBNext Ultra DNA Library Prep Kit for Illumina® (New England BioLabs). Prepared libraries were quantified using KAPA Library Quantification Kits for Illumina® platforms (KAPA Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Crude and purified proteins were further analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and its molecular weight (Broad range 11 to 245 kDa, BioLabs, England) was determined [14].
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bioRxiv - Molecular Biology 2020Quote: ... The amplicons were analyzed by 1.5% agarose gel electrophoresis with ethidium bromide staining and using a DNA ladder marker (2 log DNA ladder from New England Biolabs, USA). Amplicon bands were observed under UV light ...
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bioRxiv - Synthetic Biology 2023Quote: ... After confirming the PCR product size with gel electrophoresis the PCR product was incubated with DpnI (NEB, Ipswich, MA, Cat #R0176S) for 1 hour at 37°C to digest the plasmid template ...
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bioRxiv - Microbiology 2020Quote: ... we separated the PCR products from the parental genomic DNA by gel electrophoresis and purified the PCR fragments using the Monarch DNA Gel Extraction Kit (New England Biolabs, Evry, Fr). The purified fragments were then mixed and fused together using assembly PCR carried out with the Q5 high-fidelity DNA polymerase ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA purification by gel electrophoresis was followed by EcoRI and HindIII restriction digestion and cohesive end ligation (T4 DNA Ligase, NEB Cat. No. M0202S) for cloning ...
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bioRxiv - Molecular Biology 2021Quote: ... The digested products were separated by 0.8% agarose gel electrophoresis and purified using the Monarch® DNA Gel Extraction Kit (New England Biolabs, Ipswich, MA, USA). The purified products were ligated using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... Approximate size of the cloned fragments was assessed on agarose gel electrophoresis after double endonuclease digestion with XbaI and XhoI (New England Biolabs, Ipswich, MA, USA). The Sanger sequencing of the termini of inserted metagenomic fragments of each purified fosmid was done at Macrogen Ltd ...
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bioRxiv - Genomics 2022Quote: ... The successful amplification of a COI gene fragment of approximately 680 bp was confirmed by agarose gel electrophoresis before the amplicon was purified with the DNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) and sequenced (Eurofins Genomics ...
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bioRxiv - Plant Biology 2024Quote: ... Amplified genes were purified by agarose gel electrophoresis and extraction with the Monarch DNA Gel Extraction Kit (New England Biolabs, Ipswich, MA, USA). Both the synthesized genes and PCR amplified genes were then inserted into a doubly digested BamHI/XhoI pET28b(+ ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... DNA purity and concentrations were determined by UV260/280 spectrophotometry and gel electrophoresis via plasmid digestions using the digestion enzymes EcoRI and XhoI (New England Biolabs # R3101S and R0146S respectively).
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bioRxiv - Genomics 2023Quote: ... The DNA length and RNA absence were assessed by 2% agarose gel electrophoresis (50 V, 90 min) including the Quick-Load 1 kb DNA Ladder (New England Biolabs Inc., Ipswich, USA; Text S1). The extracted gDNA was not fragmented and larger than 10 kb (Fig ...