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Citations for New England Biolabs :
1 - 28 of 28 citations for QT Nickel Plating Process Agent For Removing Copper Impurity since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... 20 units of DpnI (NEB; for removing template plasmid), and 5μl of 10x-CutSmart buffer (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... after removing Cas9 with Restriction Endonucleases: NheI (NEB R0131L) and BamHI-HF (NEB R3136L) ...
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bioRxiv - Molecular Biology 2020Quote: ... removing DNA overhangs with the Quick Blunting™ Kit (NEB), and circularization with T4 Ligase (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The process involved using SalI-HF (New England Biolabs, cat. R3138S) and AatII (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... pGEM-N-SNAPf was first constructed by removing SNAPf from pSNAPf (NEB, N9183S) with AgeI-XhoI and inserting it into the AgeI-XhoI site of pGEM-HE (51) ...
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bioRxiv - Systems Biology 2023Quote: ... The tagmentation process was promptly neutralised using the Monarch PCR & DNA Cleanup Kit (NEB). The samples were then eluted in a final volume of 20 μL using the Elution buffer.
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bioRxiv - Bioengineering 2021Quote: ... The sample was chilled to halt the denaturation process and GlycoBuffer 2 (New England Biolabs), NP-40 ...
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bioRxiv - Molecular Biology 2022Quote: ... CaMKAR was isolated from soluble lysate by nickel chromatography NEBExpress columns (NEB). Soluble protein was quantified by Pierce BCA assay (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was cloned into pCP20 digested with PstI and EcoRV (removing the ampicillin resistance cassette) using Gibson Assembly (NEB; protocol as described by the manufacturer) ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA libraries were prepared by removing the ribosomal RNA with NEBNext rRNA depletion kit (New England Biolabs) and NEBNext Ultra II Directional RNA Library Prep kit for Illumina (New England Biolabs) ...
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bioRxiv - Bioengineering 2021Quote: A standard PCR process was performed using Q5 high-fidelity DNA polymerase from NEB (cat. no. M0491S). All PCR primers were purchased from IDT (Supplementary Table S1) ...
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bioRxiv - Biochemistry 2024Quote: ... The transcription process was driven by T7 polymerase using HiScribe T7 High Yield RNA Synthesis Kit (NEB). After 2h transcription ...
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bioRxiv - Genetics 2022Quote: ... the sgRNA sequences were inserted into the pBSMVγPDS plasmid after removing the PDS fragment by PacI and NotI digestion (New England BioLabs, Ipswich ...
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bioRxiv - Cell Biology 2021Quote: ... Nluc-CD63 construct was obtained by removing RFP sequence from RFP-CD63 (kind gift of Walther Mothes) using AgeI and XhoI restriction enzymes (New England Biolabs) and by replacing it with Nluc sequence using the same enzymes ...
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bioRxiv - Cancer Biology 2022Quote: ... we used Addgene Plasmid #48140 as a transient expression vector backbone by removing Cas9 and GFP with EcoRI-AgeI digestion (NEB), and inserting three overlapping gBlock dsDNA fragments for JAK1 ...
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bioRxiv - Immunology 2022Quote: ... Control EGFR and EGFR-CXCR5 were generated by removing the CER from the above construct using the NEBuilder HiFi DNA Assembly (New England Biolabs). CER 104 and CER 131 were generated by adding the human DAP 12 signaling domain ( GenBank™ accession number NP_001166985 ...
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bioRxiv - Biochemistry 2021Quote: The Saccharomyces cerevisiae Mtr4ΔF was constructed by removing residues 667-813 and inserting a three-glycine linker using the Q5 Site-Directed Mutagenesis Kit (NEB). All Mtr4 proteins were recombinantly expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL (Stratagene ...
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bioRxiv - Biophysics 2024Quote: ... The expression plasmid for CbpF mutant Δ142-149 was prepared from pASK-IBA2C-CbpF by removing the DNA sequence that codes for the loop using KLD mutagenesis (New England Biolabs). All sequences were verified by Sanger sequencing (LGC Genomics) ...
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bioRxiv - Biochemistry 2024Quote: ... The Mtr4ΔFist was constructed as previously described (26) by removing residues 667-813 and inserting a three-glycine linker using the Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Cell Biology 2020Quote: ... which was linearized by removing the EGFP coding sequence by enzymatic digestion (NotI and BsrGI, New England BioLabs, Ipswich, MA, USA) and was purified with the QIAquick Gel Extraction Kit (QIAGEN AG ...
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bioRxiv - Biochemistry 2020Quote: ... (DU67916) was made by removing the HA and replacing with Twinstrep 3C using Q5 site-directed mutagenesis (New England Biolabs E0552S) in pcDNA5D FRT/TO HA SARS-CoV-2 Nsp3 743-1072 (PL-pro ...
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bioRxiv - Molecular Biology 2021Quote: ... The Sox2 promoter was cloned by first removing the Ef1a promoter from the 3-SB-EF1-PBBAR-SB vector using NdeI (NEB, R0111) and SalI (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was subsequently sent for either library preparation and RNA-sequencing to Genewiz or cDNA preparation via a two-step process beginning with Lunascript RT Supermix Kit (#E3010, New England Biolabs). qRT-PCR was performed on cDNA from samples for genes of interest using the Luna Universal qPCR Master Mix (#M3003 ...
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bioRxiv - Neuroscience 2021Quote: ... The NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used to process the samples according to the protocol NEB #E7420S/L (New England Biolabs) to fragments of 300-500 bps ...
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bioRxiv - Biochemistry 2024Quote: ... DNA libraries were prepared from cDNA using a two-step PCR process (Q5 Hot Start High-Fidelity DNA Polymerase, New England Biolabs). PCR 1 used Step-1 Forward primer and Step-1 Reverse primer ...
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bioRxiv - Biophysics 2024Quote: The extracted genomic DNA was then subjected to NGS library preparation through a two-step PCR process using Q5 High-Fidelity 2× Master Mix (New England Biolabs). The first PCR step involved amplifying the genomic loci and attaching adapter sequences (primers listed in Table S10) ...
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bioRxiv - Genetics 2022Quote: Half of ChIP-enriched DNA or one sixth of input DNA were used as starting materials for DNA end repair process for library construction with NEBNext UltraTM End repair/dA-Tailling Module (NEB, E7442). Then the products were ligated to adapters (NEBNEXT Multiplex Oligos for Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were mixed with a 6X loading buffer without SDS and reducing agents (BioLabs, B7025S) and without boiling loaded onto Native-PAGE gels (NativePAGE ...