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Citations for New England Biolabs :
1 - 42 of 42 citations for Magnesium Chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 6 mM Magnesium Sulfate (MgSO4 – NEB #B1003), 1.4 mM deoxynucleotide (dNTP ...
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bioRxiv - Biochemistry 2020Quote: ... Purified embryo mRNA was fragmented to 100-200 nt RNA fragments by magnesium-buffer based degradation using the NEBNext Magnesium RNA Fragmentation Module (NEB, E6150S). Fragmentation was optimized for time and RNA input amount monitoring RNA size using the mRNA Pico Chip (Agilent ...
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bioRxiv - Plant Biology 2022Quote: ... and was fragmented using Magnesium RNA Fragmentation Module (NEB) at 86 °C for 7 min ...
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bioRxiv - Genomics 2020Quote: ... was fragmented using NEBNext Magnesium RNA fragmentation module (NEB, USA) for 1 minute at 94°C ...
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bioRxiv - Genetics 2019Quote: ... a NEBNext Magnesium RNA Fragmentation Module (New England Biolabs, E6150) was used to linearize the circRNAs in preparation for sequencing ...
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bioRxiv - Microbiology 2020Quote: ... 45 s using the NEBNext Magnesium RNA Fragmentation Module (NEB) followed by RNA purification with the Zymo RNA Clean & Concentrator kit ...
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bioRxiv - Neuroscience 2022Quote: ... 1.2 µl 10 mM zinc chloride (New England Biolabs) was included ...
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bioRxiv - Genomics 2019Quote: RNA fragmentation was done using the NEBNext Magnesium RNA Fragmentation Module (NEB, #E6150S) with incubation time at 94°C for one or two minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... and fragmented for 30 min using the NEBNext Magnesium RNA Fragmentation Module (NEB). Deep sequencing libraries for RNAseq and RP samples were prepared exactly following previously published protocols (Döring et al. ...
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bioRxiv - Immunology 2020Quote: ... After fragmentation of the RNA in a magnesium buffer (NEB Next RNA fragmentation buffer), the RNA fragments were purified using Agencourt RNAClean XP beads ...
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bioRxiv - Genomics 2022Quote: ... Extracted RNA was fragmented using the NEBNext Magnesium RNA Fragmentation module kit (NEB, USA), heating for 2min ...
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bioRxiv - Molecular Biology 2022Quote: ... then RNA was fragmentated to ~150 nt by magnesium RNA fragmentation buffer (New England Biolabs) for 4 min at 94°C ...
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bioRxiv - Immunology 2024Quote: ... Then the poly(A) RNA was fragmented into small pieces using Magnesium RNA Fragmentation Module (NEB) under 86°C for 7 min ...
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bioRxiv - Genomics 2020Quote: Fragmentation of plasma RNA was done by using an NEBNext Magnesium RNA Fragmentation Module (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... A first round of PCR was performed using 1× Standard-Taq magnesium free reaction buffer pack (NEB) with 2 mM MgCl2 (NEB) ...
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bioRxiv - Plant Biology 2023Quote: ... 2µg of polyA enriched RNA was fragmented using NEBNext® Magnesium RNA Fragmentation Module (New England Biolabs) with incubation at 94°C for 1 min ...
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bioRxiv - Biophysics 2023Quote: ... we dilute the stock DNA to a final concentration of 6 mg/mL with 1/10th volume of 10× CutSmart Buffer (0.5 M Potassium Acetate, 0.2 M Tris-acetate, 0.1 M Magnesium Acetate; New England BioLabs), 0.1% Tween (to prevent non-specific interactions) ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM calcium chloride and 200 unit/ml final concentrations of micrococcal nuclease (NEB). Cell lysates were incubated at 25 ℃ for 15 minutes before adding 3 mM final concentration of EGTA was added.
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bioRxiv - Molecular Biology 2023Quote: ... Samples were fragmented at 94°C for 2.75 min as per the NEBNext Magnesium RNA Fragmentation Module (NEB) with subsequent RNA purification using the Zymo RNA Clean & Concentrator kit ...
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bioRxiv - Biochemistry 2022Quote: ... 1× CutSmart buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate,100 μg/mL BSA, pH 7.9, NEB), 20 U RiboLock ...
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bioRxiv - Genetics 2020Quote: The long fractions (with and without rRNA depletion) were further processed with the NEBNext Magnesium RNA Fragmentation Module (NEB), as described in the manual ...
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bioRxiv - Cancer Biology 2023Quote: ... the poly(A)+ RNA was sheared into 100–200-nt fragments using Magnesium RNA Frag mentation Module (NEB, E6150S). A portion of poly(A)+ RNA fragment was directly used as input ...
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bioRxiv - Genomics 2022Quote: ... The long RNAs were then fragmented to 70-100 nt by using an NEBNext Magnesium RNA Fragmentation Module (94 °C for 7 min; New England Biolabs). After clean-up by using a Zymo RNA Clean & Concentrator kit (8X ethanol protocol) ...
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bioRxiv - Immunology 2022Quote: ... mRNA was fragmented into short fragments using a NEB Next Magnesium RNA Fragmentation Module (New England Biolabs, Ipswich, MA, USA) at 94°C for 5-7 minutes ...
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bioRxiv - Genomics 2021Quote: ... Purified RNA was fragmented 2-4 minutes (depending on the RNA Integrity Number) using NebNext Magnesium RNA Fragmentation Module (New England Biolabs) and once again purified with the RCC kit (Zymo Research ...
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bioRxiv - Microbiology 2021Quote: RNA-Seq library preparation from rRNA-depleted samples began by fragmenting them with the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs) for 110 s followed by ethanol precipitation ...
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bioRxiv - Molecular Biology 2022Quote: ... the RNA was fragmented at 95°C for 5 min by using a Next Magnesium RNA Fragmentation Module (New England Biolabs), and cleaned up by using a MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNAs from two biological replicates were subjected to poly(A) selection and fragmentation using Magnesium RNA Fragmentation Module (New England Biolabs). Libraries were prepared as described previously (Jin et al ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng DNase I treated total RNA or mRNA of one technical replicate was fragmentated to ~150 nt by magnesium RNA fragmentation buffer (New England Biolabs) for 4 min at 94°C ...
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bioRxiv - Plant Biology 2023Quote: ... At least 50 μg of total RNA was randomly fragmented for 125 s at 90°C to approximately 200 nt using the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs). Fragmented RNA was precipitated with 4 μl of GlycoBlue (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated RNA was fragmented to approximately 200 – 500 bases with the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs E6150S) and precipitated by adding 1/10 volume of 3M sodium acetate pH 5.5 (ThermoFisher AM9740 ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified ribodepleted RNA samples were fragmented for 3 minutes at 94°C in Magnesium RNA Fragmentation Module (New England Biolabs) and 3’ ends were dephosphorylated with T4 polynucleotide kinase (Lucigen) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1× CutSmart buffer (50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 µg/ml BSA or recombinant albumin; NEB), 0.5 mM 1,4-Dithiothreitol (DTT) ...
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bioRxiv - Biophysics 2023Quote: ... The reaction was mix using 1× CutSmart buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 μg/mL BSA; NEB) and 0.5 mM DTT (1,4-dithiothreitol ...
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bioRxiv - Plant Biology 2023Quote: ... Chemical fragmentation of ligated RNA to ≤200nt was performed using the Magnesium RNA fragmentation kit (New England Biolabs, cat#E6150S). 2ul RNA Fragmentation Buffer was added and samples were incubated at 94°C for 5 minutes followed by a transfer to ice and the addition of 2μl of RNA Stop solution ...
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bioRxiv - Neuroscience 2023Quote: ... The mRNA was fragmented using divalent cations under 94°C for 5-7min using the NEBNextTM Magnesium RNA Fragmentation Module (New England Biolabs, #E6150S). RNA fragments were reverse-transcribed using SuperScriptTM II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... the mRNA was fragmented into short fragments using divalent cations under elevated temperature (Magnesium RNA Fragmentation Module (NEB, cat. e6150, USA) under 94℃ 5-7min) ...
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bioRxiv - Microbiology 2021Quote: ... The viral RNA was extracted with phenol/chloroform and digested with the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs, Ipswich, MA) at 94°C for 2.5 min ...
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bioRxiv - Genetics 2021Quote: ... the poly (A) product was broken into pieces at 94 °C for 5-7 min using the Magnesium RNA Fragmentation Module (NEB, MA, USA). The RNA fragments were then reverse-transcribed using the SuperScript™ II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA from HEK293T cells was extracted using TRIzol and fragmented using NEBNext® Magnesium RNA Fragmentation Module (New England BioLabs, E6150S). 250µg of fragmented RNA was incubated with 250µL of m6A Ab conjugated to protein G magnetic beads Reaction Buffer for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... allowing a minimum RNA integrity number (RIN) of 7 (Schroeder et al., 2006) before fragmentation using NEBNext® Magnesium RNA Fragmentation Module (NEB, Ipswich, MA) into short fragments using divalent cations under high temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixed RNA was depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) and fragmented using NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. no. E6150S) for 5 min ...