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Citations for New England Biolabs :
1 - 42 of 42 citations for Ion Exchange Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the allelic exchange vector pMQ30 was first digested with SmaI (New England BioLabs) and ∼1.5kb flanking regions of the targeted gene were amplified using Phusion polymerase (New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... Exchange of V5/His to myc/His was performed using the Q5 site-directed mutagenesis kit (NEB #E0554) according to the manufacturer’s protocol resulting in pEF1-ZAP-S-myc/His and pEF1-ZAP-L-myc/His ...
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bioRxiv - Microbiology 2019Quote: ... Chromosomal deletion strains were constructed using the allele exchange vector pKAS32 digested with KpnI and SacI (NEB, High Fidelity). Luciferase reporters were constructed using the luciferase reporter vector pBBRlux digested with BamHI and SpeI (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... Single-amino acid exchanges were generated by Q5® site-directed mutagenesis (New England Biolabs GmbH, Frankfurt am Main) using the appropriate primer pairs which were designed by using NEBaseChanger ...
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bioRxiv - Developmental Biology 2024Quote: A Primer Exchange Reaction concatemerization reaction (1X PBS; 10 mM MgSO4; dNTP, 0.6 mM of A, C, and T, NEB-N0446S ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with media containing 3 µM SNAP-Cell block (New England BioLabs cat. # S9106S) and cells maintained at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... performed to exchange the previous gRNA sequence was achieved using a Q5 mutagenesis kit (New England Biolabs, Ipswich, Massachusetts, USA). gRNA sequences were inserted into pTREX-n-Cas9 using primers specific to the gene of interest in Supplementary Table 4 ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: The allelic exchange vector for mutation of the SphR binding site in the cerN promoter was built using HiFi assembly (NEB) from two PCR products amplified from PA14 genomic DNA and HindIII and KpnI cut pMQ30 ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: The allelic exchange vector for insertion of nLuc as part of the plcHR operon was built using HiFi assembly (NEB) from two PCR products generated from PA14 genomic DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a library preparation with the NEBNext Fast DNA Library Prep Set for Ion Torrent (NEB). cDNA libraries were sequenced on an Ion Torrent Proton.
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bioRxiv - Genomics 2021Quote: Libraries were prepared using the NEBNext Fast DNA Library Prep Set for Ion Torrent (New England Biolabs) with IonXpress Barcode Adapters (Ion Torrent ...
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bioRxiv - Immunology 2022Quote: ... Sequencing libraries were prepared using NEBNext® Fast DNA Library Prep Set for Ion Torrent™ (New England BioLabs), diluted to the same concentration and pooled ...
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bioRxiv - Neuroscience 2022Quote: ... and libraries were prepared using the NEBNext Fast DNA Library Prep Set for Ion Torrent (New England BioLabs, UK) and the Ion Xpress Barcode Adapters 1-96 Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... The P1 coding sequence of O/BY/CHA/2010 was selected as the site for exchange with the corresponding region of pO-FMDV using the restriction endonucleases AfIII and Clal (New England Biolabs, Ipswich, Massachusetts, USA); the recombinant plasmid was named prVP3/FMDV ...
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bioRxiv - Microbiology 2019Quote: ... barcoded sequencing libraries were constructed using the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent (New England Biolabs), and templated using the Ion PI Hi-Q Chef kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: Sequencing libraries were prepared using NEBNext® Fast DNA Library Prep Set for Ion Torrent(tm) Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... whereas KAPA HyperPlus libraries and libraries sequenced on the Ion Torrent PGM platform were quantified by qPCR using the NEBNext Library Quant Kit for Illumina (New England BioLabs) or the KAPA Library Quantification Kit for Ion Torrent platforms (Figure 1) ...
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bioRxiv - Microbiology 2021Quote: ... NGS libraries were generated using the NEBNext Fast DNA Library Prep Set for Ion Torrent (New England Biolabs, Hitchin, UK). Pooled samples were size selected with the LabChip XT (LabChip XT DNA 300 Assay Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... and sequenced on a NextSeq 2000 instrument (Srp2-HTP & Dbp2-HTP) or with the NEBNext Fast DNA Library Prep Set for Ion Torrent Kit (NEB) and sequenced on an Ion Torrent Proton (Life Technologies ...
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bioRxiv - Systems Biology 2020Quote: Genomic DNA libraries were prepared using the NEBnext Fast DNA Fragmentation and Library Prep Set for Ion Torrent (New England Biolabs, MA). Fragments of 450 bp size were separated and extracted with an E-Gel ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and used this as template to construct DNA libraries using a NEBNext Fast DNA Library Prep Set for Ion Torrent kit (New England Biolabs, Canada). Each sample library contained a unique barcode adapter (NEXTflex DNA Barcodes for Ion Torrent ...
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bioRxiv - Synthetic Biology 2020Quote: ... and media were obtained from New England Biolabs (NEB) unless otherwise noted ...
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bioRxiv - Evolutionary Biology 2021Quote: ... each culture was recovered in provided outgrowth media (NEB) by shaking at 37°C at 250 rpm for 1.5 hours ...
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bioRxiv - Genomics 2021Quote: Library preparation was performed with 150 ng of starting material using NEBNext Fast DNA Library Prep Set for Ion Torrent (New England Biolabs, Massachusetts, USA) and following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The obtained cDNA was used to prepare a NEBNext DNA Library Prep Set for Ion Torrent (New England Biolabs, Ipswich, MA, USA) and barcoded with the use of Ion Code Barcode Adapters (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 100 uL of room temperature SOC media (New England Biolabs) was added to the cells ...
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bioRxiv - Immunology 2019Quote: ... Pooled DNA (100 ng) was fragmented and barcoded using the NEBNext→ Fast DNA Fragmentation and Library Prep Set for Ion Torrent™ (NEB Inc, UK) and IonXpress Barcode Adapters kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... and subjected as an input for library preparation process using the NEBNext® Fast DNA Library Prep Set for Ion Torrent™ (New England Biolabs Inc. USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were labelled in full media with SNAP-Surface 549 (New England Biolabs) for 30 minutes at 37°C and imaged in RPMI without phenol red in a Nikon Eclipse Ti spinning disk confocal microscope with an ORCA-Flash 4.0 camera (Hamamatsu ...
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bioRxiv - Bioengineering 2020Quote: ... Electroporated cells were immediately transferred into pre-warmed SOC recovery media (New England Biolabs) and recovered for 1-2 hours at 37 °C with 250 rpm of shaking ...
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bioRxiv - Bioengineering 2019Quote: ... the culture media mix was TSB with 1 mg/mL BSA (New England Biolabs) and 50 μM calcein ...
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bioRxiv - Microbiology 2019Quote: ... cells recovered for three hours at 37°C in SOC media (NEB, cat. # B9020S). An aliquot was then taken to titer transformants and the remaining cells (860 µl ...
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bioRxiv - Cell Biology 2019Quote: ... the HeLa cells were kept in HeLa culture media treated overnight with 0.2 U/mL of □2-3,6,8,9 Neuraminidase (New England Biolabs) to cleave sialic acid from the cell surface glycans.
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bioRxiv - Biochemistry 2022Quote: ... Deglycosylated cell lysates or conditioned media were prepared by treatment of PNGase F (New England Biolabs). Samples containing around 30 μg proteins was loaded to SDS-PAGE ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples collected from culture media were treated with PNGase F enzyme (New England Biolabs, P0709S) to remove the N-linked oligosaccharides from the TECTA protein according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2022Quote: Lysates (20 μg) and media immunoprecipitates were diluted to 10 μL in 2X glycoprotein denaturing buffer (NEB), and boiled for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... washed once with media and released for 8 hours with 24 mM dCTP (New England Biolabs # N0441S). 2 mM of thymidine or 0.1 mg/ml of Nocodazole (Millipore-Sigma # SML1665 ...
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bioRxiv - Cell Biology 2020Quote: ... coli CJ236 was picked into 1 ml of 2YT media (Roth, 6676.2) supplemented with M13KO7 helper phage (NEB, N0315) to a final concentration of 1 × 108 pfu/ml and ampicillin (final concentration 100 µg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... 6 μM kifunensine was added to the ExpiCHO culture media at the time of transfection and Endo Hf (NEB) treatment of LC-kappa purified protein (∼10,000 U/mg protein at 4°C for 3 hours ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 30 min at 37°C in appropriate media containing 3µM of cell-permeable SNAP-Cell® 647-SiR (New England BioLabs). Cells were then rinsed twice with fresh media and incubated for 30min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in CO2-balanced pre-warmed media supplied with 1 µM O6-benzylguanine (BG)-coupled Alexa Fluor 647 (NEB, S9136S) for 15 min at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2024Quote: ... Thymidine was then removed by washing cells with culture media and blocking of existing CENP-A was performed by treatment with 10 mM SNAP-Cell® Block BTP (S9106S, NEB) for 30 min ...