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Citations for New England Biolabs :
1 - 31 of 31 citations for Corning Kanamycin Sulfate Powder since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 6 mM Magnesium Sulfate (MgSO4 – NEB #B1003), 1.4 mM deoxynucleotide (dNTP ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 0.5% sodium dodecyl sulfate) and Proteinase K (NEB). Reactions were incubated at room temperature overnight ...
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bioRxiv - Biochemistry 2022Quote: ... the pUC57-Kanamycin were also digested by restriction enzymes (NEB, Frankfurt am Main, Germany) and gel extracted using the QIAprep Gel Extraction kit (QIAGEN ...
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bioRxiv - Plant Biology 2020Quote: ... The powder was dissolved in a cold native extraction buffer (NEB: Tris HCl 100 mM pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... Heparan sulfate proteoglycans were detected after Heparinase III digest (NEB) using a monoclonal antibody (3G10 ...
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bioRxiv - Genetics 2022Quote: ... The kanamycin resistance cassette-gDNA junctions were prepared for sequencing using NEB Ultra I kit (New England Biolabs). Following adapter ligation and treatment with the NEB USER Enzyme ...
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bioRxiv - Microbiology 2021Quote: ... a kanamycin resistance gene flanked by FRT sites was amplified from pFKM1 using Q5 high-fidelity polymerase (NEB) and primers with 75 bp oligonucleotide tails homologous to the sequences up and downstream of the arn operon (KM9H9-F ...
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bioRxiv - Microbiology 2022Quote: ... of the SNAP-Tag-SCE-I-KanR shuttle sequence with a nine amino acid linker (HTEDPPVAT) and subsequent second recombination to clear the Kanamycin resistance and restore the SNAP-Tag sequence (NEB; for complete insertion sequence see Table 1) ...
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bioRxiv - Genetics 2020Quote: ... Each of them was made by assembling a synthetic gBlock DNA fragment with a PCR-amplified Kanamycin resistant backbone using NEBuilder DNA Assembly (New England Biolabs). The region to be PCR-amplified in each vector contains a gRNA scaffold followed by either a tRNA or the U6:3 promoter ...
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bioRxiv - Synthetic Biology 2021Quote: ... were amplified from pSerOTSλ and placed under the control of the glnS* promoter and assembled with the kanamycin selection marker and origin of replication from pSerOTSλ by gibson assembly using NEBuilder HiFi DNA Assembly master mix (NEB). tRNA expression cassettes (1x- ...
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bioRxiv - Microbiology 2020Quote: ... these fragments were assembled to flank a kanamycin resistance cassette via isothermal assembly using the NEBuilder HiFi DNA Assembly MasterMix (New England Biolabs) and cloned into pMRKO ...
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bioRxiv - Genetics 2023Quote: ... The primers contained 60 bases pairs of homologies for the downstream and upstream region of the arcZ gene as well as homology with the kanamycin resistance gene (ArcZkanfor and ArcZkan) PCR was carried out with the Q5 2X Master Mix (New England Biolabs). The mixture was run in the thermocycler with a denaturation temperature of 95°C ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid-carrying bacterial cells were transferred from their respective agar stabs onto agar plates containing 100 µg/mL of Ampicillin or 50µg/mL of Kanamycin and incubated overnight at 37°C (DH5alpha) and 27°C (NEB stable). Alternatively ...
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bioRxiv - Microbiology 2024Quote: ... All flanking regions and kanamycin resistance cassettes were amplified using the manufacturer’s protocol for Q5® High-Fidelity DNA Polymerase (New England Biolabs) and with primer concentrations of 10μM ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting DNA molecules were ligated into a pET28a vector (kanamycin resistance) with Instant Sticky-end Ligase Master Mix (NEB) and transformed for amplification in DH5α E ...
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bioRxiv - Molecular Biology 2023Quote: ... were grown out in LB medium with 50 µg/mL Kanamycin and then pelleted before plasmids were isolated using the Monarch Plasmid Miniprep Kit (NEB, T1010L). Plasmids were sequenced (Sanger ...
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bioRxiv - Biophysics 2024Quote: ... 55 The samples were mixed with 6 × purple loading dye without sodium dodecyl sulfate (NEB) and with 10 × TBE to make a 1 × solution ...
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bioRxiv - Plant Biology 2023Quote: ... Frozen plant tissue was ground to a powder and RNA was extracted using Monarch ® Total RNA Miniprep kits (NEB), RNA integrity and concentration were assessed by agarose gels and a Nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... 75 roots were ground to a powder using a cooled pestle and mortar and homogenized in 20 ml Nuclei Extraction buffer (NEB) (20 mM MOPS (pH 7) ...
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bioRxiv - Microbiology 2021Quote: ... 40 μL of 10 μM FISH probes in hybridization buffer (10% dextran sulfate [Sigma], 2mM vanadyl ribonucleoside complexes [#514025, NEB], 0.02% RNAse-free BSA [NEB] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted with a Sodium Dodecyl Sulfate (SDS) protocol from whole bodies and sequencing libraries were built with NEBNext DNA Library Prep Kits (New England Biolabs) by Novogene (Hong Kong).
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bioRxiv - Biochemistry 2022Quote: ... D-loops were deproteinized by adding 2 μL of 5% lithium dodecyl sulfate and 1 μL of 20 mg/mL proteinase K (New England Biolabs), and incubating the mixture at 37°C for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... 40 μL of 10 μM FISH probes in hybridization buffer (10% dextran sulfate [Sigma], 2mM vanadyl ribonucleoside complexes [#514025, NEB] ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent probes diluted in Hybridization Buffer (10% dextran sulfate, 0.1mg/ml salmon sperm ssDNA, 100 μl vanadyl ribonucleoside (NEB biolabs), 20ug/ml RNAse-free BSA ...
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bioRxiv - Developmental Biology 2020Quote: ... Probes were diluted in Hybridization Buffer (10% dextran sulfate, 0.1mg/ml salmon sperm ssDNA, 100 µl vanadyl ribonucleoside (NEB biolabs), 20ug/ml RNAse-free BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... Probes were hybridised in FISH hybridization buffer (50% formamide, 20% dextran sulfate, 2X SSC, 1 μg/μL BSA (New England Biolabs), 10 mM vanadyl-ribonucleoside complex ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% sodium dodecyl sulfate) and reverse crosslinked with proteinase K overnight at 65C and subsequently treated with RNAse A (NEB) for 1 hour at 37°C ...
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bioRxiv - Genomics 2024Quote: ... 1: 120) diluted in the hybridization buffer (10% deionized formamide, 2X SSC, 100mg tRNA, 5% dextran sulfate, 2mM VRC (NEB), 0,2mg/mL BSA) ...
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bioRxiv - Microbiology 2020Quote: ... Crude and purified proteins were further analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and its molecular weight (Broad range 11 to 245 kDa, BioLabs, England) was determined [14].
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bioRxiv - Genomics 2024Quote: ... of 0.1 μM polyT-rootV2 oligo diluted in hybridization buffer (2x SSC, 1 mg/mL yeast RNA, 10% dextran sulfate, 1:1000 Murine RNase inhibitor NEB M0314) on a parafilm sheet placed on a glass plate ...
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bioRxiv - Developmental Biology 2023Quote: ... and then hybridized overnight at 37°C in hybridization buffer (2xSSC, 10% dextran sulfate [Sigma-Aldrich, D8906], 1mg/mL yeast tRNA [Sigma-Aldrich, R8759], 2mM Vanadyl Ribonucleoside complex [New England Biolabs, S1402S] ...