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Citations for New England Biolabs :
1 - 26 of 26 citations for Bordetella Pertussis Toxin Glycerol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... glycerol-free (New England BioLabs, catalog no. P0705L) according to the manufacture’s recommended protocol ...
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bioRxiv - Synthetic Biology 2021Quote: Glycerol stocks of NiCo21(DE3) cells (New England Biolabs) transformed with each GFP variant were streaked onto LB agar plates with added ampicillin (100 μg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µl PNGaseF (P0705L, Glycerol-free, New England Biolabs) was added and incubated for one hour at 57⁰C ...
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bioRxiv - Bioengineering 2024Quote: ... 0.3 U/µL glycerol-free WarmStart RTx (NEB #M0439B-HC1), 1X Chai Green (Chai Biosciences #R01200) ...
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bioRxiv - Cell Biology 2020Quote: ... treated with 1,000 units of glycerol-free PNGaseF (NEB, Cat# P0705S), and incubated for 2-hr at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... glycerol-free peptidyl-N-glycosidase F (PNGase F) (New England Biolabs), Endoglycosidase Hf (Endo Hf ...
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bioRxiv - Bioengineering 2024Quote: ... 0.32 U/µL glycerol-free WarmStart Bst 2.0 (NEB #M0402B-BM), 0.3 U/µL glycerol-free WarmStart RTx (NEB #M0439B-HC1) ...
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bioRxiv - Immunology 2020Quote: The fusion protein was treated with glycerol free PNGase F (NEB, P0705S) in non-denaturing reaction conditions ...
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bioRxiv - Cancer Biology 2019Quote: ... 2.5mM Na2P2H2O7 and 20mM β-glycerol phosphate) and 10mM ATP (New England Biolabs) to a total volume of 50µl ...
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bioRxiv - Biochemistry 2021Quote: ... 4% Glycerol and 0.1 mM DTT) with 75 µM S-Adenosylmethionine (SAM, NEB), varying amounts ssRNA and duplex RNA (see above) ...
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bioRxiv - Biochemistry 2021Quote: Toxins were expressed with (His)6-tags at their C-termini in competent C43 (DE3) Escherichia coli cells (NEB) for HlgA and in BL21 (DE3 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... coli BL21 (DE3) were transformed with the appropriate toxin construct cloned into the SapI/BamHI sites of pTwin1 (New England Biolabs). Freshly transformed colonies were used to inoculate a 10-ml starter in LB containing 0.1mg/ml Carbenicillin and 0.03mg/ml chloramphenicol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coli BL21 (DE3) were transformed with the appropriate toxin construct cloned into the SapI/BamHI sites of pTwin1 (New England Biolabs). Freshly transformed colonies were used to inoculate a 10-ml starter in LB containing 0.1mg/ml Carbenicillin and 0.03mg/ml chloramphenicol ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% Igepal CA-630 and 5% glycerol) with 1 U/µL murine RNase inhibitor (NEB), 0.1 µg/µL T7 RNA polymerase (purified in-house ...
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bioRxiv - Systems Biology 2019Quote: ... 5 mM CaCl2 and 25% (v/v) glycerol)) containing MNase ((micrococcal nuclease (M0247S, New England Biolabs)) and supplemented with protease inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... after which 2 µL of Recombinant PNGaseF (Glycerol-free) (New England Biolabs, Ipswich, MA; catalog # P0709L) was added to each sample ...
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bioRxiv - Biochemistry 2023Quote: Removal of N-linked oligosaccharides in mini-procollagens was performed with PNGase F (New England BioLabs, glycerol-free). Ten micrograms of mini-procollagens were first denatured at 100 °C for 10 min in Glycoprotein Denaturing Buffer provided with the enzyme ...
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bioRxiv - Microbiology 2021Quote: ... 5 mM MgCl2, 10% Glycerol, 0.05% NP-40, 2 mM DTT, 10 mM Ribonucleoside-Vanadyl complex [New England Biolabs, MA USA] ...
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bioRxiv - Microbiology 2021Quote: ... 40 μL purified supernatant were combined with 2,500 units recombinant glycerol-free PNGase F and Glycobuffer 2 (New England Biolabs) following the manufacturer’s protocol for non-denaturing digestion and incubated at 37°C for 5 hours ...
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bioRxiv - Biophysics 2023Quote: ... Reaction was terminated with 3 μL of stop buffer (5% sarkosyl, 0.025% bromophenol blue, 50% glycerol) and incubated with 1.6 units of Proteinase K (NEB, P8107S) for 15 minutes at 37 degrees before resolving on 1% agarose TAE gel stained with SybrSafe (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Protein G magnetic beads were washed 2-3x with 1ml blocking buffer (20 mM Hepes, 150 mM KCl, 20% Glycerol, 0.5 % Tween, BSA (Biolabs), Heparin 0.2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Equilibration buffer (GF150 buffer supplemented with 0.1 mM Mg-ATP and 10% glycerol) was used to equilibrate 20 μL of SNAP-Capture Magnetic Beads (New England Biolabs) slurry in 1.5 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Cell Biology 2023Quote: ... Unbound material was removed by washing the samples 5x for 5min with head over head rotation at 4°C in wash buffer plus detergent (1x buffer XT, 1% digitonin, 2 mM PMSF and 10% glycerol) using a magnetic separator (New England Biolabs). Bound material was finally eluted with Biotin elution buffer (1x buffer Biotin XT elution ...
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bioRxiv - Biophysics 2021Quote: ... The surface is then passivated by incubating bovine serum albumin (BSA, New England Biolabs, 10 mg/ml in PBS and 50% glycerol) for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and lysed with 2× concentrated lysis buffer (0.25% Triton X-100, 50 mM KCL, 100 mM Tris-HCl pH 7.4, 40% glycerol, 1/50 volume of RNase inhibitor; NEB, Cat. No. M0314S) at room temperature for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB, 45% glycerol 80% and 30% dH2O). and incubated for 30 min at 37°C and for 15 min at 80°C ...