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Citations for New England Biolabs :
1 - 18 of 18 citations for Bordetella Pertussis Toxin Frozen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Frozen RNA was sent for cDNA library preparation (using NEB Next® Ultra RNA Library Prep Kit for Illumina ...
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bioRxiv - Biochemistry 2021Quote: Toxins were expressed with (His)6-tags at their C-termini in competent C43 (DE3) Escherichia coli cells (NEB) for HlgA and in BL21 (DE3 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... coli BL21 (DE3) were transformed with the appropriate toxin construct cloned into the SapI/BamHI sites of pTwin1 (New England Biolabs). Freshly transformed colonies were used to inoculate a 10-ml starter in LB containing 0.1mg/ml Carbenicillin and 0.03mg/ml chloramphenicol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coli BL21 (DE3) were transformed with the appropriate toxin construct cloned into the SapI/BamHI sites of pTwin1 (New England Biolabs). Freshly transformed colonies were used to inoculate a 10-ml starter in LB containing 0.1mg/ml Carbenicillin and 0.03mg/ml chloramphenicol ...
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bioRxiv - Genetics 2023Quote: ... Samples were homogenized frozen in 1X DNA/RNA Protection Reagent (NEB T2011L), then processed according to kit instructions including on-column DNase digestion ...
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bioRxiv - Bioengineering 2023Quote: ... Frozen cell pellets were resuspended in 4 mL of IMAC buffer (NEB, Ipswich, MA, USA) on ice and dispersed using an ultrasonic disruptor (Sonics ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from 30mg frozen tumour using the Monarch Total RNA Miniprep Kit (NEB). RNA library preparation and sequencing was performed by Novogene (UK ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from flash frozen samples with the Monarch® Genomic DNA Purification Kit (New England Biolabs), including the on-column RNase treatment ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was extracted from flash-frozen samples with the Monarch® Total RNA Miniprep Kit (New England Biolabs) including the DNase treatment step ...
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bioRxiv - Molecular Biology 2024Quote: ... fresh or frozen cell pellets were osmotically lysed in presence of 150mL of Nuclei Prep Buffer (NEB, Cat.T3052) supplemented with 5.5 mL of Rnase A (NEB ...
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bioRxiv - Genomics 2023Quote: ... Commercial and breeding program samples were extracted from frozen tissue using the Monarch Genomic DNA Purification Kit (NEB), as per manufacturer’ s instructions using the enzymatic cleanup protocol with both proteinase K and RNase A ...
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bioRxiv - Microbiology 2021Quote: ... Sorted transduced cells were frozen down for later use or subjected to EnGen mutation detection kit (New England BioLabs) for on-target gene editing confirmation ...
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bioRxiv - Microbiology 2021Quote: ... Frozen pellets from the time course sampling were normalized by OD600 for resuspension in 1x blue loading buffer (NEB) supplemented with 1x reducing agent (DTT) ...
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bioRxiv - Plant Biology 2021Quote: ... the DNA of the two parents that generated the cross was extracted from flash-frozen young leaves using NEBNext Ultra II kit (NEB) and sequenced to ~40x coverage as 150 bp reads on an Illumina MiSeq at the Biological Nanostructures Lab in the California NanoSystem Institute at UC Santa Barbara ...
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bioRxiv - Plant Biology 2023Quote: ... Frozen plant tissue was ground to a powder and RNA was extracted using Monarch ® Total RNA Miniprep kits (NEB), RNA integrity and concentration were assessed by agarose gels and a Nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: Population genomic DNA was extracted from frozen cell pellets using the Monarch Genomic DNA Purification Kit (New England Biolabs, Beverly, MA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: RNA was isolated from frozen hippocampal samples stored at -80°C using the Monarch® RNA Total Isolation kit (NEB T2010S). Hippocampus from the left hemisphere was used where possible ...
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bioRxiv - Genomics 2019Quote: The genomic DNA was extracted from 10 mg frozen brain cortex samples using NEB Monarch genomic DNA® purification kit (NEB, Ipswich, MA, USA). Four microliters of RNase A (100mg/mL ...