Labshake search
Citations for New England Biolabs :
301 - 350 of 380 citations for 6 6' Di O tert butyldimethylsilyl D lactal since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and 2U of Uracil-D-glycosylase (New England Biolabs, USA). Conditions of PCR were as follows ...
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bioRxiv - Molecular Biology 2022Quote: ... oligo-d(T)25-Magnetic Beads (S14195, New England Biolabs) were used ...
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bioRxiv - Genetics 2024Quote: ... One microliter of oligo d(T)23VN primer (50 μM) (NEB) was added to 200 ng/µL of RNA (170 ng worm RNA + 35 ng yeast RNA ...
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bioRxiv - Microbiology 2023Quote: ... mRNA molecules are captured using magnetic Oligo d(T)beads (NEB). Following purification ...
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bioRxiv - Cancer Biology 2021Quote: ... mRNAs were enriched using Oligo d(T)25 Magnetic Beads (NEB S1419S) following manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... mRNA was enriched with Oligo d(T)25 Magnetic Beads (New England Biolabs) and fragmented by heating at 94 °C for 3 min in 5× First Strand Buffer (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 15 µl of Oligo d(T)25 Magnetic Beads (S1419S, New England Biolabs), were combined with 50 µl of binding buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... pGEMHE-DEST containing TaHKT1;5-D was linearized using sbfI-HF (New England Biolabs) before synthesising cRNA using the mMESSAGE mMACHINE T7 Kit (Ambion ...
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bioRxiv - Genomics 2021Quote: ... samples were incubated with 20 μl Oligo d(T) Magnetic Beads (NEB, S1419S) in PCR strips for selection of polyadenylated (poly(A) ...
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bioRxiv - Genomics 2021Quote: ... mRNAs were incubated with Oligo d(T) Magnetic Beads (New England BioLabs S1419), then fragmented in 2x Superscript III first-strand buffer (Thermo Fisher Scientific with 10mM DTT (Thermo Fisher Scientific 18080044 ...
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bioRxiv - Genomics 2022Quote: ... mRNA was isolated using Oligo d(T)25 Magnetic Beads (New England Biolabs) and fragmented ...
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bioRxiv - Genomics 2022Quote: ... mRNAs were enriched by incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and then fragmented/eluted by incubation at 94°C for 9 min ...
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bioRxiv - Immunology 2024Quote: ... mRNAs were enriched by incubation with Oligo d(T)25 Magnetic Beads (NEB). To fragment Poly A-enriched mRNA ...
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bioRxiv - Immunology 2020Quote: PBMCs were co-emulsified with oligo d(T)25 magnetic beads (New England Biolabs) in lysis buffer (100mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... samples were incubated with 20 μl Oligo d(T)25 Magnetic Beads (NEB, S1419S) in PCR strips for selection of polyadenylated (poly(A) ...
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bioRxiv - Genomics 2024Quote: ... Poly(A) mRNA was selected using Oligo d(T)25 Magnetic Beads (NEB, S1419S) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: PBMCs were co-emulsified with oligo d(T)25 magnetic beads (New England Biolabs) in lysis buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The pENTR/D construct was then digested with the MluI restriction enzyme (NEB, Cat# R0198S) and recombined into a modified gateway destination vector harboring Hygromycin drug resistance as described in Law et al ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 µg of total RNA was used with D(T)23VN primer (New England Biolabs), 10 mM dNTPs (New England Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... Poly(A)+ RNA was purified using Oligo d(T)25 Magnetic Beads (New England Biolabs). For northern blotting ...
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bioRxiv - Cancer Biology 2024Quote: ... and WT SETD8 or T140A/D SETD8 cDNA were ligated using T4 ligase (NEB #M0202). T140A/D SETD8 cDNAs were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit according to the manufacturer’s instructions (Agilent #210518).
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bioRxiv - Genomics 2020Quote: ... Further processing for mRNA selection was performed with Oligo-d(T)25 Magnetic Beads (NEB, E7490) and the integrity of the RNA was validated once more with Agilent 4200 TapeStation (Agilent ...
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bioRxiv - Genomics 2021Quote: ... the rest was subjected to pull-down with oligo-d(T)x25 magnetics beads (NEB-S1419S), 1ml of slurry per 1L of cell culture during 1 hour at 4°C ...
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bioRxiv - Genomics 2022Quote: ... polyadenylated RNA was isolated from total RNA using oligo d(T)25 Magnetic Beads (NEB, #S1419S) at a 1μg Total RNA to 10μg beads ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... and the D-AlaR cassette (pGGF003) were combined in a ’Greengate reaction’ using BsaI-HF (NEB), T4 DNA-Ligase (Thermo Scientific) ...
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bioRxiv - Genomics 2022Quote: ... and mRNA was selected using 75 μl of Oligo d(T)25 magnetic beads (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... We extracted RNA using Monarch® Total RNA Miniprep Kit (New England BioLabs GmbH, Frankfurt, D, E2010). Material was ground in Mixer Mill 200 (Retsch GmbH ...
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bioRxiv - Plant Biology 2022Quote: ... and 3xHA::3C::BLRP tag encoding DNAs were respectively ligated into pENTR/D constructs using AscI (NEB) digestion site in the pENTR/D vector ...
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bioRxiv - Genomics 2024Quote: ... 30 μg per replicate of poly-adenylated mRNA was isolated using oligo d(T) beads (NEB E7490L). Resulting mRNA was split into 6 separate reactions and cDNA was synthesized using a gene-specific primer (5’-CTCATCAATGTATCTTATCATGTCTG-3’ ...
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bioRxiv - Neuroscience 2020Quote: ... Poly(A)+ RNAs were enriched from total RNAs using Oligo d(T)25 magnetic beads (New England Biolabs), according to the manufacturer’ s instructions ...
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bioRxiv - Immunology 2020Quote: The second PCR product (V, D, J heavy genes) was cloned in frame by HiFi assembly (NEB, UK) into the KpnI/PstI (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... This was followed by one round poly(A) purification using oligo d(T) magnetic beads (New England Biolabs). 1.5-2 µg of mRNA was fragmented to 100-150 nts using RNA Fragmentation Reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... polyA mRNA was enriched by two successive rounds of incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and then fragmented/eluted by incubation at 94°C for 9 min ...
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bioRxiv - Biochemistry 2022Quote: Native 3’ end extension assays experiments where set-up as describe in the D-loop assays with the exception that dNTP’s (1 mM) and Klenow (exo-)(NEB) were added after 10 minutes of D-loop formation ...
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bioRxiv - Cell Biology 2020Quote: ... Additional S/A and S/D mutations were generated by using Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from total RNA of 10-d-old seedlings using M-MuLV reverse transcriptase (New England Biolabs); each gene was amplified using specific primers (see Supplemental Table 2 ...
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bioRxiv - Immunology 2021Quote: ... except after the Ni-NTA step it was concentrated (1 mg/ml in 0.2 ml) and shaved with endoglycosidase D (10 μl, 100 unit) (New England BioLabs) for 16 hrs at 4°C prior to gel filtration ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µL of RNase H (5,000 U/mL) (New England Biolabs, Fig. 2f and Extended Data Fig. 2c,d) were treated with 1.5 of 10× RNase H buffer in the sample by adjusting with Milli-Q H2O to total 15 µL ...
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bioRxiv - Genomics 2023Quote: ... The poly-A+ fraction of RNA was isolated using Oligo d(T)25 Magnetic Beads (New England BioLabs Inc.) following the commercial protocol for Mammalian Cells provided by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼40 µg of bulk RNA was subjected to poly(A) selection using Oligo d(T)25 magnetic beads (NEB) followed by on-bead 5’ ligation of a biotinylated REL5 linker sequence using T4 RNA ligase 1 (NEB ...
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bioRxiv - Plant Biology 2024Quote: Mutations (D to V) of the MHD motif were carried out using inverse PCR with primers containing AarI (NEB) or BsmBI (NEB ...
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bioRxiv - Developmental Biology 2024Quote: Polyadenylated RNA was purified from the DNase-treated total RNA using oligo d(T)25 magnetic beads (NEB, S1419), and then used for the library preparation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was reverse transcribed into cDNA using the LunaScript RT Master Mix Kit and d(T)23VN primers according to manufacturer’s instructions (New England Biolabs). Negative controls lacking the reverse transcriptase (RT ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (3 μg) was applied for poly(A) mRNA purification by using oligo-d(T) magnetic beads (S1419S, NEB). RNA fragmentation ...
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bioRxiv - Biochemistry 2021Quote: ... 1000 base pairs upstream and downstream from the clpP2 gene were amplified by PCR using the A–B and C–D primer pairs from table 3 (Phusion polymerase, GC buffer, New England Biolabs). The PCR products were purified with E.Z.N.A ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from HEK293T cells using Bio Tri RNA reagent (Bio-lab) and mRNA was captured using Oligo d(T)25 magnetic Beads (NEB) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: AP-DNA was prepared by incubating 50 μM 7-mer ssDNA [d(GTCUGGA]] with 10 U of uracil DNA glycosylase (UDG, New England Biolabs) in UDG Buffer at 37 °C for 1.5 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was submitted to mRNA Isolation and RNA fragmentation with NEBNext Oligo d(T)25 beads (NEW ENGLAND BioLabs) and cDNA synthesis ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Combinations of indexed Ion Torrent primers were used to amplify barcodes in the library for each time-point (Figure S5 D) using Phusion High-Fidelity PCR (New England BioLabs). To avoid sampling bias during PCR amplification ...
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bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction was isolated from 4.5 μg of DNAse-treated total RNA using NEBNext Oligo d(T)25 Magnetic beads kit (NEB, USA), according to manufacturer’s recommendations ...