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Citations for New England Biolabs :
1951 - 2000 of 4087 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... miRE-Rv (5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’) and Q5 Hot Start High-Fidelity DNA polymerase (NEB M049S). The final amplicon was then digested and cloned into LT3GEPIR in-between XhoI and EcoRI restriction sites as previously described2 ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing Cdt1 were further purified on a 5 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Cancer Biology 2024Quote: ... All ngRNAs were cloned into pLKO.5 Puro-2A-RFP between BsmBI (New England Biolabs) cut sites ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The 10-µl reactions contained: 5 µl Luna Universal qPCR Master Mix (New England Biolabs), 0.25 µl forward primer (10 µM) ...
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bioRxiv - Biochemistry 2024Quote: ... and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs) and self-ligated by T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of LongAmp Taq 2x master mix (New England Biolabs, Ipswich, Massachusetts, United States), and 2.5 µl of the PCR product as template ...
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bioRxiv - Plant Biology 2024Quote: ... dephosphorylated F4 (5 µM) was incubated with γ-[32P]-ATP and T4 polynucleotide kinase (NEB) for 45 min at 37°C ...
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bioRxiv - Genomics 2024Quote: ... DNA was circularized at a concentration of 5 ng/µl with T4 DNA ligase (NEB), and treated with a cocktail of exonucleases ...
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bioRxiv - Molecular Biology 2023Quote: ... 55 Excess adaptor was removed by incubation with 5′ deadenylase and the exonuclease RecJf (NEB). TIS11B protein-RNA complexes were eluted from the beads by heating at 70°C for one minute ...
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bioRxiv - Cancer Biology 2023Quote: ... 1pmol ssDNA substrate 5’ DY782 ATTATTATTATTATTATTATTTCATTTATTTATTTATTTA-3’ (Eurofins, UK) and 0.75U uracil-DNA glycosylase (NEB) were added to 10µg protein lysate at 37°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Nef deletion Reverse: 5’-AGATCTACAGCTGCCTTGTAAGTCATTGG-3’) using Q5® Site-Directed Mutagenesis Kit (NEB #E0554S).
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bioRxiv - Molecular Biology 2023Quote: ... Probes were synthesized as ssDNA oligos and 5’ 32P-radiolabeled with T4 polynucleotide-kinase (NEB) and ψ-32P-ATP (6000 Ci/mmol ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′ Iq competent Escherichia coli (NEB, Cat# C2992H) and extracted using the PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Molecular Biology 2023Quote: RNA was denatured for 5 min at 95°C in 1x RNA Loading Dye (NEB) and separated on denaturing polyacrylamide gels (12% ...
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bioRxiv - Cell Biology 2023Quote: ... using 5 μl of the reaction with 1 μl of loading dye (New England Biolabs). Agarose gel was electrophoresed at 70V until resolution of bands required was reached ...
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bioRxiv - Molecular Biology 2022Quote: ... and Phusion DNA polymerase after 20 cycles of amplification (1.2×10−5) (NEB, product literature). Because the error rate from the RNA polymerase was much higher than the RT and PCR steps ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified using a Monarch PCR & DNA Cleanup Kit (5 μg) from NEB and then visualized on an 0.8 to 1.2 % percent agarose gel resolved in Tris-acetate-EDTA electrophoresis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... dynein coated beads were incubated with 5 μM SNAP-Cell TMR (New England BioLabs, #S9105S) in the column for 10 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2022Quote: ... qPCR was performed using 5 µL of 2X Luna Universal qPCR Master Mix (NEB, #M3003E), 2 µL of 10 µM primer pair mix and 2 µL of 5 ng/µL cDNA per reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... the binding reaction was added to 200 μl of 5% (v/v) amylose beads (NEB) that has been blocked overnight with 1 mg/ml BSA and 0.5 mg/ml tRNA in 20 mM HEPES ...
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bioRxiv - Genetics 2023Quote: ... DNA was circularized at a concentration of 5 ng/μL with T4 DNA ligase (NEB), and treated with a cocktail of exonucleases ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... the isolated RNA was treated with 1 μl (5 units) RppH enzyme (New England Biolabs) in NEB Buffer 2 (50 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were amplified in chemically competent 5-alpha F’Iq Escherichia coli cells (New England Biolabs) after 42°C heat shock transformation and extracted using the GenElute HP Plasmid Miniprep Kit (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... Ligation was performed by increasing the volume 5 times with T4 DNA ligase buffer (NEB) to a final concentration of 1X ...
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bioRxiv - Microbiology 2023Quote: ... The 5’ ends were radiolabelled with 20 μCi γ32P-ATP using T4 polynucleotide kinase (NEB) and separated from free nucleotides using a MicroSpin G-50 column (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... These cells that were pre-stained with 5 µM SNAP-Surface 647 (New England Biolabs) for one hour at room temperature if necessary were stained with 5µg/mL of Alexa488 labeled lectins (PNA ...
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bioRxiv - Genetics 2024Quote: All plasmids were transformed into NEB 5-alpha Competent Escherichia coli cells (New England Biolabs) and purified using Qiaprep Spin Miniprep Kits or Plasmid Plus Maxi Kits for plasmid libraries (QIAGEN) ...
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bioRxiv - Biochemistry 2024Quote: In-vitro synthesized pri-miRNAs (100 pmol) were 5’-end labelled by T4-PNK (NEB) using [γ-32P]-ATP (Perkin Elmer ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified RNA (5 μg) was 5ʹ phosphorylated by incubating with T4 polynucleotide kinase (PNK, NEB) in 1 mM ATP at 37 °C for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... The 5’-end adapter (/5Phos/NNNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT/3SpC3/) was first adenylated using Mth RNA Ligase (NEB), purified using Oligo Clean & Concentrator (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... followed by 5 uL of DNAase I (2000 U/mL, New England BioLabs, Cat. M0303S). Samples were then treated with 50 uL of NEB buffer for a 30-minute incubation at 37°C followed by a 10-minute incubation at 74°C to inactive the enzymes ...
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bioRxiv - Immunology 2024Quote: ... and 0.8U/µL of Klenow Fragment (3’-5’ exo-) DNA polymerase (NEB, Cat No. M0212M). Nickase induced linear SDA was performed using 3nM (0.04U/ µL ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were diluted 1:1 and 1:5 respectively in puc19 vector (New England Biolabs). HaloTag expression was confirmed using HaloTag ligand dyes HTL-AF488 ...
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bioRxiv - Biochemistry 2024Quote: ... In short 5’-phosphorylated and 3’-OH RNA was circularized with RNA ligase 1 (NEB) followed by denaturing PAGE purification as described above ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 μg of plasmid DNA of ACT1 was linearized using SpeI restriction enzyme (NEB, USA), purified by Phenol:Chloroform extraction and re-suspended in 10 μl of RNase free sterile water (Raut et al. ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µg of RNA was used to synthesize cDNA by oligo dT (New England Biolabs) and reverse transcriptase (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... and was [32P]-labelled at the 5’-end using T4 polynucleotide kinase (New England BioLabs) and purified as previously described (55) ...
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bioRxiv - Molecular Biology 2024Quote: The 5′ end labelling of RNA was obtained with T4 polynucleotide kinase (New England Biolabs) and [γ32P] adenosine triphosphate as previously described [59] ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription of the endogenous MYC 5’UTR was done with Induro Reverse Transcriptase (NEB) and a reverse primer targeting the coding sequence proximal to the ATG ...
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bioRxiv - Biochemistry 2024Quote: ... the following DNA nucleotide probes were 5’-end 32P-ATP radiolabeled using T4 PNK (NEB): as listed in the Key Resources Table.
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bioRxiv - Genetics 2024Quote: ... The assembled products were transformed into NEB 5-α electrocompetent Escherichia coli cells (NEB, C2989K) by electroporation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and subsequently incubated in 5% normal goat serum (NGS; New England BioLabs, Hitchin, Hertforshire, UK) diluted in 0.1 M phosphate-buffered saline (PBS ...
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bioRxiv - Biophysics 2024Quote: ... The ligation mix was then transformed into NEB 5-alpha competent cells (NEB, Cat. # C2987H) and colonies were selected using 50 µg/mL Kanamycin LB agar plate ...
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bioRxiv - Cell Biology 2024Quote: ... and one set of samples were treated with 5 units of TEV protease (NEB, #P8112) for 30 min on ice ...
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bioRxiv - Genetics 2024Quote: ... The ligated products were electroporated into NEB 5-α electrocompetent Escherichia coli cells (NEB, C2989K), and millions (1000× of pgRNA library size ...
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bioRxiv - Genetics 2024Quote: ... 5 μg of purified plasmid was digested with 250 units of ApaI restriction enzyme (NEB) for 1 hour at 37° ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μg DNA spiked with methylation standards was digested with SmaI endonuclease (NEB) at 25°C for 8 h ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... Q5-high Fidelity 2× master mix was used as polymerase from NEB (# M0429L). PCR condition for the 1st PCR was ...
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bioRxiv - Developmental Biology 2021Quote: ... 200 ng of PCR products in 1X NEB buffer 2 (New England Biolabs) were hybridized under the following conditions ...