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Citations for New England Biolabs :
51 - 100 of 3370 citations for D Iditol 1 13C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... polyA mRNA was enriched by two successive rounds of incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and then fragmented/eluted by incubation at 94°C for 9 min ...
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bioRxiv - Cell Biology 2020Quote: ... Additional S/A and S/D mutations were generated by using Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from total RNA of 10-d-old seedlings using M-MuLV reverse transcriptase (New England Biolabs); each gene was amplified using specific primers (see Supplemental Table 2 ...
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bioRxiv - Genomics 2023Quote: ... The poly-A+ fraction of RNA was isolated using Oligo d(T)25 Magnetic Beads (New England BioLabs Inc.) following the commercial protocol for Mammalian Cells provided by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼40 µg of bulk RNA was subjected to poly(A) selection using Oligo d(T)25 magnetic beads (NEB) followed by on-bead 5’ ligation of a biotinylated REL5 linker sequence using T4 RNA ligase 1 (NEB ...
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bioRxiv - Plant Biology 2024Quote: Mutations (D to V) of the MHD motif were carried out using inverse PCR with primers containing AarI (NEB) or BsmBI (NEB ...
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bioRxiv - Developmental Biology 2024Quote: Polyadenylated RNA was purified from the DNase-treated total RNA using oligo d(T)25 magnetic beads (NEB, S1419), and then used for the library preparation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was reverse transcribed into cDNA using the LunaScript RT Master Mix Kit and d(T)23VN primers according to manufacturer’s instructions (New England Biolabs). Negative controls lacking the reverse transcriptase (RT ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (3 μg) was applied for poly(A) mRNA purification by using oligo-d(T) magnetic beads (S1419S, NEB). RNA fragmentation ...
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bioRxiv - Biochemistry 2021Quote: ... 1000 base pairs upstream and downstream from the clpP2 gene were amplified by PCR using the A–B and C–D primer pairs from table 3 (Phusion polymerase, GC buffer, New England Biolabs). The PCR products were purified with E.Z.N.A ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from HEK293T cells using Bio Tri RNA reagent (Bio-lab) and mRNA was captured using Oligo d(T)25 magnetic Beads (NEB) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: AP-DNA was prepared by incubating 50 μM 7-mer ssDNA [d(GTCUGGA]] with 10 U of uracil DNA glycosylase (UDG, New England Biolabs) in UDG Buffer at 37 °C for 1.5 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was submitted to mRNA Isolation and RNA fragmentation with NEBNext Oligo d(T)25 beads (NEW ENGLAND BioLabs) and cDNA synthesis ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Combinations of indexed Ion Torrent primers were used to amplify barcodes in the library for each time-point (Figure S5 D) using Phusion High-Fidelity PCR (New England BioLabs). To avoid sampling bias during PCR amplification ...
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bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction was isolated from 4.5 μg of DNAse-treated total RNA using NEBNext Oligo d(T)25 Magnetic beads kit (NEB, USA), according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... and an equimolar mix of primers P5-IR2a-d primer (10 µM) in a reaction with 50 ng of adaptor ligated template and Phusion DNA polymerase (NEB) in a thermocycler with the following program 98°C 3 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 25 μg of total RNAs from 12-day-old Arabidopsis leaves were extracted using the Trizol method and incubated with 30 μl of pre-equilibrated oligo d(T)25 magnetic beads (NEB) for 15 minutes at room temperature with continuous rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... and mRNA fragments with a poly(A) tail were isolated by Oligo d(T)25 Magnetic Beads (NEB Biolab, S1419S). Illumina sequence adaptors were added during reverse transcription with SMRT methods (Wang et al. ...
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bioRxiv - Systems Biology 2024Quote: ... first a highly active HIS4-targeting sgRNA was cloned into D-227 vector by digesting the plasmid with BbVCI enzyme (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... 1000 bp directly upstream and downstream of the HIS4-targeting sgRNA on the genome was PCR amplified and cloned on the upstream and downstream of the pENO1-Cas9-PptefT on D-227 vector using New England BioLabs (NEB) NEBuilder® HiFi DNA Assembly Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... Polyadenylated RNA was then isolated from the whole-cell RNA using NEB Oligo d(T)25 Magnetic beads (NEB, S1419S) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2023Quote: ... and eukaryotic mRNA was removed by binding and discarding the eukaryotic mRNA polyA region to oligo d(T)25 magnetic beads (England Biolabs). Finally ...
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bioRxiv - Plant Biology 2023Quote: The PCR products and the pENTR-D-TOPO vector were digested using the restriction enzymes NotI-HF y AscI (New England BioLabs) following the instructions of the supplier and the digested vector was dephosphorylated by TSAP enzyme to avoid its recircularization (Promega ...
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bioRxiv - Microbiology 2023Quote: ... These PCRs were performed with the specified primer sets (a, c and e or b, d and f) and Q5 High-Fidelity Polymerase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... mRNAs were enriched by incubation with Oligo d(T) Magnetic Beads (New England Biolabs, Cat. No. S1419S, Ipswich, MA, USA) and then fragmented/eluted by incubation at 94°C for 9 min ...
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bioRxiv - Molecular Biology 2022Quote: Polyadenylated RNA was purified from the DNase-treated total RNA using the oligo d(T)25 magnetic beads (NEB, S1419) and used for the library preparation ...
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bioRxiv - Biophysics 2023Quote: Oligonucleotides used as substrates in D-loop assays were radiolabelled at their 5′-end using T4 Polynucleotide Kinase (T4 PNK - NEB) and adenosine triphosphate [γ-32P] (Perkin Elmer ...
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bioRxiv - Molecular Biology 2023Quote: ... was isolated from total RNA or crude cell lysates by 2 rounds of affinity chromatography using oligo d(T)25-derivatized magnetic beads (NEB). RNA yields were quantified by ultraviolet (UV ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% Nonidet P-40) and incubated with pre-washed anti-D-lactyllysine antibody-conjugated protein A agarose beads (PTM Biolabs) at 4 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... amplicons were assembled as in-frame fusions with emGFP PCR product into a pLenti6/V5-D-TOPO vector using NEBuilder HiFi DNA Assembly (New England Biolabs). cDNA sequences were confirmed by Sanger Sequencing (Australian Genome Research Facility ...
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bioRxiv - Genetics 2024Quote: ... mRNA was isolated via two rounds of purification with Dynabeads Oligo d(T)25 according to the manufacturer (New England Biolabs) from 5 flies or 10 head/thoraces.
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bioRxiv - Genetics 2024Quote: ... mRNA was isolated from the total RNA using Oligo-d(T)25 Magnetic Beads (New England Biolabs, Ipswich, MA, USA), fragmented by heat and magnesium ...
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bioRxiv - Cell Biology 2021Quote: ... 500ng-1 μg total RNA was enriched in poly-A tailed RNA transcripts by double incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and fragmented for 9 min at 94°C in 2X Superscript III first-strand buffer containing 10mM DTT (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... and BGH reverse (5’-TAG AAG GCA CAG TCG AGG -3’) primers from the pcDNA3.1+/-C-(K)-D vector using standard methods (NEB 2x Q5) which include 5-10 ng DNA per reaction ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 ng total RNA was enriched for poly-A tailed RNA transcripts by double incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and fragmented for 9 min at 94°C in 2X Superscript III first-strand buffer containing 10 mM DTT (Invitrogen ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The mRNA was then isolated through two rounds of poly-A selection using the oligo-d(T)25 beads (NEB #S1419S). The RNA was digested with nuclease P1 (Wako USA #145-08221 ...
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bioRxiv - Molecular Biology 2022Quote: ... depletion was achieved by taking the unbound supernatant after chRNA was incubated with the oligo-d(T) beads from the NEBNext Poly(A) mRNA magnetic isolation module (NEB, E7490L). Then ...
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bioRxiv - Neuroscience 2024Quote: ... RNA isolation from C20 cells was carried out as described above and total RNA was enriched in poly-A tailed RNA transcripts by double incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and fragmented for 9 minutes at 94°C in 2X Superscript III first-strand buffer containing 10 mM DTT (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... flanking and targeting exon 3 (2nd coding exon) of gasdermin d (Q9D8T2) were designed and templates for transcription were derived by PCR using Q5-Polymerase (Biolabs, M0491S). Transcription was performed using the HiScribeT7 kit (Biolabs ...
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bioRxiv - Pathology 2022Quote: ... Total RNA was subjected to two rounds of poly(A) selection using oligo-d(T)25 magnetic beads (New England Biolabs, CA, USA). Illumina compatible RNAseq library was prepared using NEB next ultra RNAseq library preparation kit ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted using TRIzol from 14 d old seedlings and poly(A) enriched before library preparation (NEB E7490 and E7765). Libraries were sequenced on Illumina Hiseq 2500 in a 2×100 bp format ...
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bioRxiv - Biochemistry 2021Quote: ... 1000 base pairs upstream and downstream from the C-terminus of clpP1 were amplified by PCR using the A–B and C–D primer pairs from Table 2 (Phusion polymerase, GC buffer, New England Biolabs, Ipswich, United States). For clpP2 ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of Exonuclease 1 (NEB, M0293S) was added to each PCR product and incubated at 37C for 15 min to digest any single-stranded product ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× NEB buffer 1 (NEB, B7001S) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1% cholorophorm was added together with 10 μg mL-1 DNase 1 (NEB) and 1 μg mL-1 RNase A ...
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bioRxiv - Molecular Biology 2024Quote: ... The four PCR products were then assembled in a 1:1:1:1 ratio using HiFi assembly (NEB, E5520S) according to the manufacturer’s instructions to create the pLenti-STARR vector (pAS5018) ...
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bioRxiv - Microbiology 2022Quote: The recombinant constructs (pETDuet-1-TaPHB-1 and pETDuet-1-TaPHB-1-RUVBL1 were separately transformed into E. coli Lemo21(DE3) (NEB) chemical competent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Unincorporated ddRTP molecules were inactivated by adding 1 µL of Buffer 2 (80 mM MgCl2) and 1 µL of 1:1 rSAP (NEB):50% glycerol ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μL T4 RNA Ligase 1 (NEB, M0437M), 1 μL SuperAseIn (Invitrogen AM2694) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM MgCl2) supplemented with 1 mM ATP and 1 U/μL RNase Inhibitor (NEB). Reaction was incubated for 1 hour at 37°C ...