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Citations for New England Biolabs :
551 - 600 of 2189 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... GGGMcm3 (in association with the other Mcm2-7 subunits) was cleaved off the resin with 500 units of 7×His-TEV protease (NEB; rotation overnight at 4°C). The flow-through was collected and applied to 0.4 mL volume Ni-NTA Agarose resin (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... were added to each sample in a 9-cycle PCR reaction prepared by adding 12.5 μl Phusion 2X Master Mix (NEB) and 2 μl each of Nextera XT S5 and N7 adapters ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µl was added to a 9 µl Luna® Universal One-Step RT-qPCR reaction (NEB, Cat# E3005L). RT-qPCR was carried out to manufacturer specifications in a CFX96 Touch Real Time PCR Machine (Bio-Rad) ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 9 cDNA libraries were prepared with an NEBNext Ultra II RNA Library Prep kit (NEB, cat:E7770S) and sequenced with an Illumina HiSeq4000 ...
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bioRxiv - Bioengineering 2020Quote: ... Then 5 μl extracted RNA was fragmented at 94°C for 9 minutes by using 0.2× fragmentation buffer (NEB) before conducting the nanoswitch detection ...
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bioRxiv - Cell Biology 2024Quote: ... the sequence encoding a sgRNA targeting the 3’ end of arkx was assembled by annealing two complementary oligonucleotides (gRNA_ARKx_a and gRNA_ARKx_b) and cloned into the BsaI-digested pHF-gC backbone (9) using T4 DNA ligase (New England Biolabs #M0202). The donor plasmid pD_ARKx_icKD was produced by assembling four DNA fragments in a Gibson Assembly reaction (77 ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed from 2.5 ng of DNA and prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina with 9 PCR cycles (NEB #E7645S, New England Biolabs). Library quality was assessed by High Sensitivity DNA Assay on an Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... Qualifying samples (RNA Integrity Number ≥ 9) were then prepped following the standard protocol for the NEB next Ultra ii Stranded mRNA (New England Biolabs). Sequencing was performed on the Illumina NextSeq 500 with Paired End 42bp × 42bp reads ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR was carried out with primers targeting UPF1 exons 9 and 12 using Q5® High-Fidelity DNA Polymerase (NEB) (Table S5) ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Transcription reactions were set up with RNA NTPs (5 mM each ATP, CTP, UTP, 9 mM GTP) (NEB, Ipswitch, MA), 0.004 U/µL Thermostable Inorganic Pyrophosphatase (NEB ...
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bioRxiv - Genomics 2022Quote: ... PCR enrichment of adaptor ligated DNA was performed for 9 cycles using the NEBNext Multiplex Oligos for Illumina (NEB #E7600S) kit to add Illumina dual index sequences ...
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bioRxiv - Physiology 2022Quote: ... 1 L of diluted extracts were incubated with 9 ml of packed amylose resin (catalog no. E8021S, New England Biolabs) and incubated at 4°C for 2h with gentle rotation ...
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bioRxiv - Microbiology 2021Quote: ... Amplification-free indexed Illumina libraries were prepared (9) using the NEBNext Ultra II DNA Library Prep kit (New England BioLabs). The libraries were quantified using the Accuclear Ultra High Sensitivity dsDNA Quantitative kit (Biotium ...
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bioRxiv - Microbiology 2021Quote: ... primers that contained restriction sites for BamHI and XmaI or XbaI (Table S5, primers No. 9-16 and 95-98) and the high-fidelity polymerase Q5 (New England Biolabs) according to vendor’s manual ...
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bioRxiv - Microbiology 2020Quote: ... before resuspending the beads in 25 μl RNA ligation mix (9 μl H2O, 3 μl 10x T4 RNA ligase buffer (NEB), 0.3 μl 0.1M ATP ...
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bioRxiv - Evolutionary Biology 2021Quote: ... CDS plus 7–9 bp of untranslated sequence were amplified using High Fidelity Phusion Taq (New England BioLabs, NEB, USA), 3% DMSO vol/vol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... CDS plus 7–9 bp of untranslated sequence were amplified using High Fidelity Phusion Taq (New England BioLabs, NEB, USA), 3% DMSO vol/vol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1B) driving intestinal Pun-PGP-9 expression were assembled using the NEB HIFI DNA Assembly Kit (New England Biolabs Inc.) according to the manufacturer’s instructions into the supplied pUC19 vector linearized by SmaI (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were generated by 7-9 rounds of PCR amplification using NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter ...
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bioRxiv - Systems Biology 2024Quote: ... We digested ∼2ug of each of 24 barcoded ORF plasmid pools (2 replicate pools of each of 9 hORFs and 3 vORFs) overnight with I-SceI (NEB) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... and PCR amplified for 7-9 cycles depending on the samples using NEBNext High Fidelity 2× PCR master mix (New England Biolabs). The optimal cycle number was determined empirically each time by qPCR ...
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bioRxiv - Genomics 2024Quote: ... Following end repair with NEBNext® End Prep Ultra II Kit (Supplemental Table 9, Cat No. E7645, NEB, Ipswich, MA), custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix (Cat No ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA samples with RQN > 9 were used for mRNA isolation with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). cDNA libraries were then prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Biophysics 2024Quote: ... Positively supercoiled DNA was prepared by incubating with 9°N Reverse Gyrase (5 units/μg DNA, M0200, provided by New England Biolabs) in 35 mM Tris-HCl (pH 7.5 at 25 °C) ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μL GlycoBuffer 2 (10 ×) (NEB), 10 μL 10% NP-40 (NEB) ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μL 10% NP-40 (NEB), 10 μL PNGase F (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL MnCl2 (10 mM NEB), 10 µL phosphatase buffer (10x PMP buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was quality checked using Bioanalyser (RIN>9) and libraries generated using NEBNext Ultra II Library Prep Kit (NEB, E7770) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmid was linearized by PCR and a gene block introducing 9 alanine point mutations was ordered from IDT and cloned into the linearized plasmid by Gibson assembly (NEB E2611L) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos for side 1 and 2 were dimerized separately by mixing 9 μl of OligoA at 100 μM with 9 μl of OligoB at 100 μM and 2 μl of 10x DNA Ligase Buffer (NEB, M0202S) and heating to 95 °C for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... adapter ligation and PCR amplification (9 cycles) was performed using NEBNext Ultra II directional RNA library prep kit for Illumina protocol (NEB, E7760) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 volumes 10 mM MnCl2 and 10 volumes of 10x PMP buffer (NEB). The phosphatase mix for comparing untreated with treated samples contained 1:20 λ-phosphatase in 1 mM MnCl2 and 1x PMP-buffer ...
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bioRxiv - Cell Biology 2020Quote: ... This was immediately followed by ligation of Illumina adaptors and PCR amplification (for 9-12 cycles) using Illumina barcoding primers and Phusion DNA polymerase (NEB, Cat# M0530S). PCR products were double size-selected using the KAPA Pure beads (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... and AAV9-based rep-cap plasmids incorporating individual heptameric peptides (EC1-10) (pACG2-[EC1-10], pACG2-QuadYF+TV-[EC1-10], and pACG9-[EC1-10]) were generated through site-directed mutagenesis (NEB Q5 site-directed mutagenesis kit ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μl 10× T4 Ligase Buffer (NEB; # B0202S), 1 μl dATP solution 100 mM (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... In total 27 libraries (3 species x 9 time points) were prepared according to NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, U.S.A.) followed by paired end sequencing (100bp x 2 ...
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bioRxiv - Genomics 2021Quote: ... followed by with a buffer adapted from (5-9) consisting of 0.1% (1mg/mL) BSA (New England Biolabs, Ipswich, MA, Item No. B9000S) / 0.3% Igepal CA 630 (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... Amplicons prepared for long-Illumina read sequencing (PE300) underwent end repair with NEBNext® End Prep Ultra II Kit (Supplemental Table 9, Cat No. E7645, NEB, Ipswich, MA). Custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix with a 15 min incubation at 20°C (Supplemental Table 6) ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed from 2.5 ng of DNA and prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina with 9 PCR cycles (NEB #E7645S, New England Biolabs). Library quality was assessed by High Sensitivity DNA Assay on an Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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bioRxiv - Genetics 2024Quote: ... 10 µL 10 mg/mL BSA (NEB cat no. B9000s), 840 μL DNA grade H2O (Invitrogen Waltham ...
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bioRxiv - Genomics 2021Quote: 10 μg genomic DNA from transfected mESCs were digested using 10 μl 10 U/μL NlaIII (NEB, #R0125S) in a 50 μL final volume for 3 hours at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Molecular Biology 2021Quote: ... in 50 μl of total reaction volume (10 μl 5X KAPA buffer, 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer ...
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bioRxiv - Molecular Biology 2022Quote: ... in 50 μl of total reaction volume (10 μl 5X KAPA buffer, 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Molecular Biology 2022Quote: ... in 50 μl of total reaction volume (10 μl 5X KAPA buffer, 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 mM MgCl2) and 10 U Klenow (exo-) polymerase (NEB, M0212S) and α-32P-dCTP (Perkin Elmer ...
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bioRxiv - Cancer Biology 2020Quote: ... The ligation reaction (10 μL) consisted of 10 units of BbsI (NEB), 600 units of T4 DNA ligase (NEB) ...