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Citations for Protean :
1 - 41 of 41 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
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bioRxiv - Cell Biology 2021Quote: ... with a constant voltage of 100 V for 1 h (Mini Protean Tetra Cell). The immuno-detection was performed by a SNAP i.d ...
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bioRxiv - Bioengineering 2019Quote: ... PAGE separation was carried out in 1 mm vertical mini gel format (Bio-Rad Mini-PROTEAN Tetra Cell) with a discontinuous buffer system under denaturing conditions ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in buffer containing 1% NP-40 and proteins were separated using SDS-PAGE (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad), transferred to low fluorescence PVDF membranes (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... 75 μg of total proteins were separated by 15 % SDS–polyacrylamide gel electrophoresis and afterwards transferred onto polyvinylidene difluoride membranes using a transblot transfer chamber (X-Cell SureLock™, Invitrogen Novex Mini-cell, or Mini-PROTEAN Tetra Cell, Biorad). Western blots were probed with anti-MART-1 antibodies (1:1000) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µg of extracted proteins mixed with 2× SDS sample buffer were separated on 10% SDS PAGE gel (Mini PROTEAN TGX Precast Gel ...
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bioRxiv - Biophysics 2020Quote: ... Inputs (2 μl) and elutions (10 μl) samples were analyzed by 4-15% SDS-PAGE (MINI-PROTEAN TGX stain-free, Bio-Rad) and staining with Quick Coomassie (Generon ...
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bioRxiv - Neuroscience 2023Quote: ... in a wet chamber (Mini-PROTEAN II cell (BIO-RAD)) filled with transfer buffer (25mM Tris base ...
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bioRxiv - Cell Biology 2022Quote: ... in a vertical tank (Mini-PROTEAN® Tetra Cell, Bio-Rad). After transfer ...
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bioRxiv - Plant Biology 2022Quote: ... using a wet blot system (Mini-PROTEAN Tetra Cell, Bio-Rad) in Tris-Gly buffer with 10 % (v/v ...
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bioRxiv - Synthetic Biology 2021Quote: ... with the gel apparatus (Mini-PROTEAN Tetra Cell, BioRad, Hercules, CA). The mating sample and perchlorate sample were diluted to equalize the total amount of protein load per well to a total of 12.5 μg ...
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bioRxiv - Genetics 2019Quote: ... at 300V for 15min (Mini PROTEAN® Tetra Cell, BIO-RAD). The total protein marker was UV-activated for 105 seconds (Fisher Biotech 312nm Transilluminator ...
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bioRxiv - Microbiology 2023Quote: ... in a vertical tank (Mini-PROTEAN® Tetra Cell, Bio-Rad). After transfer ...
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bioRxiv - Neuroscience 2024Quote: ... using a mini-gel apparatus (Bio-Rad Mini Protean II cell, Milano, Italy). Proteins were than electroblotted on Immuno PVDF membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... IPG strips were loaded onto 12% SDS-PAGE gels (PROTEAN Plus Dodeca Cell, BioRad). Gels were scanned for fluorescence as above and stained with Coomassie Blue followed by densitometry ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated by SDS-PAGE (Bio-Rad, Mini-PROTEAN® Tetra cell, #1658000) and transferred onto nitrocellulose membranes (Bio-Rad #1704158 ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates were separated by SDS-PAGE (Bio-Rad 4-20% Mini-PROTEAN TGX Precast Gel) and proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysates were analyzed by 12% SDS-PAGE for 80 min at 100 V (Mini-Protean Cell ...
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bioRxiv - Biochemistry 2023Quote: ... The runs were performed at 10 V cm-1 (Mini-PROTEAN spacer plate, Bio-Rad) for 45 minutes (or until loading dye reached the bottom of the gel ...
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bioRxiv - Bioengineering 2020Quote: ... 1D separation was run according to the O’Farrell protocol63 (adapted for Bio-Rad Mini-PROTEAN Tube Cell). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ... 2D separation was run according to the Laemmli protocol64 (adapted for Bio-Rad Mini-PROTEAN Tetra Cell). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Whole-cell lysates were separated on SDS-PAGE on 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Whole-cell lysates were subjected to SDS–PAGE on 4–20% gradient gels (Mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Whole-cell lysates were subjected to SDS–PAGE on 4–20% gradient gels (Mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: Denatured total cellular protein samples (95°C, 5 min) from cells or brain tissue were separated on SDS polyacrylamide gels (4–15% Mini-PROTEAN® TGX Stain-Free™ gels ...
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bioRxiv - Microbiology 2022Quote: All native electrophoresis gels were run in a Tetra Vertical Electrophoresis Chamber (Mini-PROTEAN® Tetra Cell, Bio-Rad, CA, USA). Recombinant PNPase (100 pmols ...
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bioRxiv - Bioengineering 2021Quote: ... A sample containing 1-20µg of protein was loaded into a 4-15% precast gel (Bio-Rad Mini-ProTEAN) and run at 60V for 20min followed by 160V for 1 hour ...
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bioRxiv - Synthetic Biology 2019Quote: ... The purity and size of the proteins were validated on SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20° C ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μg of cell lysates were separated on a 4-20% resolving gel (Bio-Rad Mini-PROTEAN TGX Precast Gel #456-1094), transferred to a PVDF membrane for analysis using anti-FLAG (Sigma Aldrich #F3165 ...
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bioRxiv - Bioengineering 2019Quote: Free FAM NHS ester remaining after purification was quantified by polyacrylamide gel electrophoresis (PAGE) run according to the Laemmli protocol51 (adapted in BioRad Mini-PROTEAN Tetra Cell manual). Briefly ...
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bioRxiv - Neuroscience 2019Quote: ... boiled for 1 min and an equivalent of ∼1.5 brains was immediately loaded onto a 10% acrylamide gel for SDS-PAGE at 80V (Mini-Protean Cell, BioRad, Hercules, CA). Separated proteins were blotted onto nitrocellulose membranes at 20 V for 6 minutes using an iBlot system (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... The purity and size of the proteins were validated on a SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20° C.
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bioRxiv - Synthetic Biology 2023Quote: ... The purity and size of the proteins were validated on an SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at 4° C.
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bioRxiv - Microbiology 2022Quote: ... The pellet obtained was dissolved in 10 μL of 20 mM Tris-HCl (pH 7.2) and 10 μL of Laemmli sample buffer (Mini-PROTEAN® Tetra Cell-Bio-Rad). The solubilized proteins were heated (95 °C ...
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bioRxiv - Microbiology 2023Quote: ... One microliter of each sample was mounted on a layer of 1.2% (w/v) agarose in 1:3 (vol/vol) TSB/PBS placed on a glass plate (Bio-Rad Mini-PROTEAN Short Plate) with a coverslip placed on top of each sample ...
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bioRxiv - Plant Biology 2023Quote: Protein samples of whole cell extracts (0.5 µg chlorophyll or 10 µg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Plant Biology 2024Quote: Protein samples of whole cell extracts (5 μg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, US) and blotted onto nitrocellulose membranes ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µg of each protein sample was separated in one dimension using 4-20% tris-glycine gels (Mini-PROTEAN TGX, Bio-Rad Laboratories, Inc.). A 1:6 dilution of the PageRuler Plus pre-stained protein ladder (10 µl ...
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bioRxiv - Biochemistry 2023Quote: ... different amounts of TTR1st in the range 0.1 1−1.10 μg were loaded in a native 4-15% PAGE gel (Mini-PROTEAN® TGX™ Precast Gel, Bio-Rad). The gel contained colored molecular weight standards (Precision Plus Protein Dual Color Standards ...
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bioRxiv - Synthetic Biology 2023Quote: ... The reactions were run at 125 V for 2.5 hours on a denaturing (6 M urea) 20% (w/v) polyacrylamide gel in 50 mM NaOAc pH 5.2 running buffer (using the Biorad Mini- PROTEAN tetra gel, 1 mm gel thickness). The gels were stained with Sybr Gold (Invitrogen ...