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Citations for Froggabio :
1 - 34 of 34 citations for rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... real-time PCR was performed using the SensiFAST SYBR No-ROX Kit (Cat. No BIO-98005, FroggaBio) using a Rotor-Gene 6000 (Corbett Life Science) ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was reverse transcribed into single-stranded cDNA using the SensiFAST cDNA Synthesis Kit for RT-PCR (Cat. No BIO-65054, FroggaBio). After conversion to cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of both cell lines was subjected to mycoplasma testing (e-Myco™ VALiD Mycoplasma PCR Detection Kit, Froggabio, 25239) and confirmed negative.
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-qPCR was completed using SensiFAST™ SYBR® No-ROX Kit (FroggaBio BIO-98020). Optimal annealing temperatures for each primer were tested in temperature gradients followed by a dilution series to determine primer efficiencies ...
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bioRxiv - Genetics 2020Quote: ... PCR was conducted using the 2×Taq FroggaMix kit (#FBTAQM, FroggaBio) in the S1000 thermal cycler (Catalog # 184-0048 ...
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bioRxiv - Cell Biology 2022Quote: ... Detection was performed with Western Lightning® Plus-ECL substrate (NEL103001EA, FroggaBio) and exposed to x-ray film (sc-201697 ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR analysis was carried out using the Lo-Rox SYBR 2x mix (FroggaBio Inc #CSA-01195) under the following conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... All other qRT-PCR analyses were conducted using cDNA mixed with SensiFAST SYBR Green PCR Master Mix (FroggaBio) and primers described in Table 2 ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was performed using SensiMix SYBR No-Rox master mix (Froggabio, Toronto, Canada) on a QuantStudio5 instrument (Applied biosystems ...
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bioRxiv - Synthetic Biology 2023Quote: ... we amplified the cassette by PCR using Taq (Froggabio T-500) or Q5 (NEB M0491 ...
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bioRxiv - Cell Biology 2024Quote: ... Regular PCR was performed using 2X Taq FroggaMix (Froggabio, Concord, ON, Canada) with gene-specific primers (Table 1).
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bioRxiv - Biochemistry 2021Quote: ... and positive transformants were identified by colony PCR using FroggaBio Taq DNA polymerase (FroggaBio). Plasmid sequence was confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was verified on a 1% agarose gel with a 100 bp ladder (Froggabio) for reference.
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bioRxiv - Genetics 2020Quote: ... We used 2.5 μL of isolated genomic DNA in 25 μL PCR reactions (2X FroggaMix; FroggaBio) utilizing primers flanking the guide target regions at ∼400 bp upstream and downstream of the sgRNA target sequences ...
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bioRxiv - Genetics 2019Quote: ... We used 2.5 μL of isolated genomic DNA in 25 μL PCR reactions (2X FroggaMix; FroggaBio) utilizing primers flanking the guide target regions at ~400 bp upstream and downstream of the sgRNA target sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR amplification of the Jak3 pseudokinase domain was performed using MyTaq HS Red Master Mix (FroggaBio) and primers FWD ...
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bioRxiv - Genetics 2019Quote: ... PCR was performed with primers P243 and P472 and separated on 2% agarose gels stained with 0.05% Redsafe (FroggaBio). See Supplementary Dataset 1 for primer sequences.
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bioRxiv - Plant Biology 2023Quote: ... was performed on an Agilent AriaMx RealTime PCR instrument using the SensiFAST SYBR No-ROX qPCR 2X master mix (FroggaBio, Canada) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and DNA isolation kits were from FroggaBio (Concord, Canada), and all laboratory chemicals were from Bioshop Canada (Burlington ...
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bioRxiv - Physiology 2024Quote: ... using SensiFAST SYBR No-ROX Kit (Cat no. BIO-98050, Bioline, Froggabio, Canada). The amplification reaction contained 1 μL of diluted cDNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the plasmid DNA was extracted from bacteria using a plasmid extraction kit (FroggaBio) to validate the plasmid single mutant libraries ...
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bioRxiv - Evolutionary Biology 2021Quote: ... plasmid DNA was extracted from each pool with a standard miniprep kit (FroggaBio, Concord, Canada) using 1.5 ml of bacterial suspension as input and eluting in 50 μL kit elution buffer (10 mM Tris-HCl pH 8.5) ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized from 1 µg RNA using SensiFAST cDNA Synthesis Kit (FroggaBio; Concord, Canada). qPCR was performed using a QuantStudio™ 7 Flex Real-Time PCR System in conjunction with a SybrGreen System (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were routinely tested for mycoplasma using the e-myco VALID mycoplasma testing kit (FroggaBio, 25239). Cells were maintained at 37°C temperature and 5% CO2 in a humidified atmosphere.
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... and first-strand cDNA was generated using the SensiFast cDNA Synthesis kit (FroggaBio, DD-BIO-65053). Quantitative PCR was then performed using PowerUP SYBR™ Green PCR (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The resulting plasmids were then purified using the Geneaid High-Speed Mini Kit (Froggabio, Concord, ON) and then sequenced at the Université Laval sequencing center ...
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bioRxiv - Microbiology 2022Quote: ... RNA extractions were performed using either a Presto Mini RNA Yeast Kit from FroggaBio (cat. RBYD050) or an RNeasy Mini Kit from Qiagen (cat ...
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bioRxiv - Molecular Biology 2019Quote: ... genomic DNA from yeasts cells was extracted by using Geneaid-Presto Mini gDNA Yeast Kit from FroggaBio Scientific Solutions following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: RNA was isolated from a pool of 10 embryos using The GENEzol™ TriRNA Pure Kit (FroggaBio). RNA was then directly used in the Luna® Universal One-Step RT-qPCR Kit (New England BioLabs) ...
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bioRxiv - Systems Biology 2020Quote: ... the plasmid DNA was extracted from bacteria cells following transformation using a mini-prep plasmid extraction kit (FroggaBio). Yeast genomic DNA extraction of the library of ABP1 SH3 mutants was performed using a standard phenol/chloroform protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was extracted from a pool of five heads for each treatment using the GENEzol™ TriRNA Pure Kit (Froggabio). RNA samples were DNase treated using the Invitrogen™ DNA-free™ DNA Removal Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... a 10 μl qPCR reaction is set up using SensiFAST SYBR No-ROX kit (FroggaBio/Bioline, catalogue number BIO-98050), forward and reverse primers (Table 2) ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was extracted from cultured cells using a commercial kit following the manufacturer’s instructions (Genaid, Froggabio or Nagel-Macherey). One μg of total RNA was reverse-transcribed as per the manufacturer’s instructions (VWR and BioRad) ...
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bioRxiv - Developmental Biology 2024Quote: RNA was isolated from a pool of 10 embryos at 50% epiboly using the GENEzol™ TriRNA Pure Kit (FroggaBio). RNA samples were DNase treated using the Invitrogen™ DNA-free™ DNA Removal Kit (Thermo Fisher Scientific ...