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Citations for Froggabio :
1 - 19 of 19 citations for hsa mir 30b RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... PCR was performed with primers P243 and P472 and separated on 2% agarose gels stained with 0.05% Redsafe (FroggaBio). See Supplementary Dataset 1 for primer sequences.
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bioRxiv - Neuroscience 2023Quote: ... mRNA was reverse transcribed into single-stranded cDNA using the SensiFAST cDNA Synthesis Kit for RT-PCR (Cat. No BIO-65054, FroggaBio). After conversion to cDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... a 10 μl qPCR reaction is set up using SensiFAST SYBR No-ROX kit (FroggaBio/Bioline, catalogue number BIO-98050), forward and reverse primers (Table 2) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-qPCR was completed using SensiFAST™ SYBR® No-ROX Kit (FroggaBio BIO-98020). Optimal annealing temperatures for each primer were tested in temperature gradients followed by a dilution series to determine primer efficiencies ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR analysis was carried out using the Lo-Rox SYBR 2x mix (FroggaBio Inc #CSA-01195) under the following conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... All other qRT-PCR analyses were conducted using cDNA mixed with SensiFAST SYBR Green PCR Master Mix (FroggaBio) and primers described in Table 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was amplified using primers specific to different genetically engineered alleles (Supplementary Table 1) using FroggaMix (FroggaBio FBTAQM). PCR conditions started with 95°C for 3 min ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was performed using SensiMix SYBR No-Rox master mix (Froggabio, Toronto, Canada) on a QuantStudio5 instrument (Applied biosystems ...
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bioRxiv - Genetics 2020Quote: ... PCR was conducted using the 2×Taq FroggaMix kit (#FBTAQM, FroggaBio) in the S1000 thermal cycler (Catalog # 184-0048 ...
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bioRxiv - Synthetic Biology 2023Quote: ... we amplified the cassette by PCR using Taq (Froggabio T-500) or Q5 (NEB M0491 ...
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bioRxiv - Cell Biology 2024Quote: ... Regular PCR was performed using 2X Taq FroggaMix (Froggabio, Concord, ON, Canada) with gene-specific primers (Table 1).
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bioRxiv - Biochemistry 2021Quote: ... and positive transformants were identified by colony PCR using FroggaBio Taq DNA polymerase (FroggaBio). Plasmid sequence was confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was verified on a 1% agarose gel with a 100 bp ladder (Froggabio) for reference.
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bioRxiv - Genetics 2020Quote: ... We used 2.5 μL of isolated genomic DNA in 25 μL PCR reactions (2X FroggaMix; FroggaBio) utilizing primers flanking the guide target regions at ∼400 bp upstream and downstream of the sgRNA target sequences ...
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bioRxiv - Genetics 2019Quote: ... We used 2.5 μL of isolated genomic DNA in 25 μL PCR reactions (2X FroggaMix; FroggaBio) utilizing primers flanking the guide target regions at ~400 bp upstream and downstream of the sgRNA target sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR amplification of the Jak3 pseudokinase domain was performed using MyTaq HS Red Master Mix (FroggaBio) and primers FWD ...
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bioRxiv - Neuroscience 2023Quote: ... real-time PCR was performed using the SensiFAST SYBR No-ROX Kit (Cat. No BIO-98005, FroggaBio) using a Rotor-Gene 6000 (Corbett Life Science) ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of both cell lines was subjected to mycoplasma testing (e-Myco™ VALiD Mycoplasma PCR Detection Kit, Froggabio, 25239) and confirmed negative.
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bioRxiv - Plant Biology 2023Quote: ... was performed on an Agilent AriaMx RealTime PCR instrument using the SensiFAST SYBR No-ROX qPCR 2X master mix (FroggaBio, Canada) according to the manufacturer’s protocol ...