Labshake search
Citations for Froggabio :
1 - 26 of 26 citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... GenJet Plus transfection reagent was from FroggaBio. μ-Slide 8-well chambered polymer coverslips were from Ibidi ...
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bioRxiv - Cell Biology 2022Quote: ... according to LipoD293 reagent instructions (FroggaBio cat #SL100668.1): Lentiviral packaging plasmids pMD2.g (Addgene #12269) ...
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bioRxiv - Molecular Biology 2019Quote: ... NP-40 detergent was from Biovision and PolyJet DNA transfection reagent was from FroggaBio (Toronto, Ontario, Canada). All other reagents were from Sigma-Aldrich unless otherwise specified ...
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bioRxiv - Microbiology 2021Quote: ... Viruses were produced in HEK293 using Polyjet In Vitro Transfection Reagent (FroggaBio, Cat#SL100688). HEK293 cells were seeded at a density of 106 cells/mL in 6-well plates in complete media and were transfected with 3 µg of pDNA after cells had reached 70% confluence ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were transfected into CHO-K1 cells grown to 80-90% cell confluency using the transfection reagent PolyJetTM (FroggaBio, Concord Ontario), according to the manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... and DNA isolation kits were from FroggaBio (Concord, Canada), and all laboratory chemicals were from Bioshop Canada (Burlington ...
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bioRxiv - Genetics 2020Quote: ... PCR was conducted using the 2×Taq FroggaMix kit (#FBTAQM, FroggaBio) in the S1000 thermal cycler (Catalog # 184-0048 ...
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bioRxiv - Physiology 2024Quote: ... using SensiFAST SYBR No-ROX Kit (Cat no. BIO-98050, Bioline, Froggabio, Canada). The amplification reaction contained 1 μL of diluted cDNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the plasmid DNA was extracted from bacteria using a plasmid extraction kit (FroggaBio) to validate the plasmid single mutant libraries ...
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bioRxiv - Evolutionary Biology 2021Quote: ... plasmid DNA was extracted from each pool with a standard miniprep kit (FroggaBio, Concord, Canada) using 1.5 ml of bacterial suspension as input and eluting in 50 μL kit elution buffer (10 mM Tris-HCl pH 8.5) ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized from 1 µg RNA using SensiFAST cDNA Synthesis Kit (FroggaBio; Concord, Canada). qPCR was performed using a QuantStudio™ 7 Flex Real-Time PCR System in conjunction with a SybrGreen System (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-qPCR was completed using SensiFAST™ SYBR® No-ROX Kit (FroggaBio BIO-98020). Optimal annealing temperatures for each primer were tested in temperature gradients followed by a dilution series to determine primer efficiencies ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were routinely tested for mycoplasma using the e-myco VALID mycoplasma testing kit (FroggaBio, 25239). Cells were maintained at 37°C temperature and 5% CO2 in a humidified atmosphere.
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... and first-strand cDNA was generated using the SensiFast cDNA Synthesis kit (FroggaBio, DD-BIO-65053). Quantitative PCR was then performed using PowerUP SYBR™ Green PCR (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The resulting plasmids were then purified using the Geneaid High-Speed Mini Kit (Froggabio, Concord, ON) and then sequenced at the Université Laval sequencing center ...
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bioRxiv - Microbiology 2022Quote: ... RNA extractions were performed using either a Presto Mini RNA Yeast Kit from FroggaBio (cat. RBYD050) or an RNeasy Mini Kit from Qiagen (cat ...
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bioRxiv - Molecular Biology 2019Quote: ... genomic DNA from yeasts cells was extracted by using Geneaid-Presto Mini gDNA Yeast Kit from FroggaBio Scientific Solutions following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... real-time PCR was performed using the SensiFAST SYBR No-ROX Kit (Cat. No BIO-98005, FroggaBio) using a Rotor-Gene 6000 (Corbett Life Science) ...
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bioRxiv - Developmental Biology 2024Quote: RNA was isolated from a pool of 10 embryos using The GENEzol™ TriRNA Pure Kit (FroggaBio). RNA was then directly used in the Luna® Universal One-Step RT-qPCR Kit (New England BioLabs) ...
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bioRxiv - Systems Biology 2020Quote: ... the plasmid DNA was extracted from bacteria cells following transformation using a mini-prep plasmid extraction kit (FroggaBio). Yeast genomic DNA extraction of the library of ABP1 SH3 mutants was performed using a standard phenol/chloroform protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was extracted from a pool of five heads for each treatment using the GENEzol™ TriRNA Pure Kit (Froggabio). RNA samples were DNase treated using the Invitrogen™ DNA-free™ DNA Removal Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... a 10 μl qPCR reaction is set up using SensiFAST SYBR No-ROX kit (FroggaBio/Bioline, catalogue number BIO-98050), forward and reverse primers (Table 2) ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was reverse transcribed into single-stranded cDNA using the SensiFAST cDNA Synthesis Kit for RT-PCR (Cat. No BIO-65054, FroggaBio). After conversion to cDNA ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was extracted from cultured cells using a commercial kit following the manufacturer’s instructions (Genaid, Froggabio or Nagel-Macherey). One μg of total RNA was reverse-transcribed as per the manufacturer’s instructions (VWR and BioRad) ...
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bioRxiv - Developmental Biology 2024Quote: RNA was isolated from a pool of 10 embryos at 50% epiboly using the GENEzol™ TriRNA Pure Kit (FroggaBio). RNA samples were DNase treated using the Invitrogen™ DNA-free™ DNA Removal Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of both cell lines was subjected to mycoplasma testing (e-Myco™ VALiD Mycoplasma PCR Detection Kit, Froggabio, 25239) and confirmed negative.