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Citations for Bioline :
1 - 43 of 43 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Escherichia coli DH5α (Bioline) was used for cloning and E ...
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bioRxiv - Microbiology 2019Quote: ... Escherichia coli DH5α (Bioline) were used for cloning and grown in lysogeny broth (LB ...
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bioRxiv - Biophysics 2021Quote: ... coli α-Select (Bioline, UK) was used for general plasmid storage and propagation ...
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bioRxiv - Microbiology 2019Quote: ... coli Silver α select cells (Bioline) were used for cloning and were grown in LB medium at 37°C with 25 μg/ml kanamycin or 10 μg/ml tetracycline when appropriate.
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bioRxiv - Synthetic Biology 2020Quote: ... Escherichia coli α-Select (Bioline, UK) was used as a host for plasmid propagation ...
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bioRxiv - Microbiology 2020Quote: ... coli α-select silver efficiency (Bioline) was used to manipulate the pGEM-TEasy library of gene cassettes ...
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bioRxiv - Neuroscience 2019Quote: ... A nested PCR (Table S2) using MyTaq™ HS Red Mix (Bioline; United States) was necessary for a sufficient amplification of a 920 bp region flanking the gRNA1 target site ...
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bioRxiv - Genomics 2021Quote: ... coli cells (NEB C2987 or Bioline BIO-85027). Bacterial clones were prepared for plasmid extraction using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... coli cells (NEB C2987 or Bioline BIO-85027). Bacterial clones were prepped using a midiprep kit ...
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bioRxiv - Genetics 2023Quote: ... coli cells (NEB C2987 or Bioline BIO-85027). Bacterial clones were prepped with a midiprep kit ...
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bioRxiv - Microbiology 2020Quote: ... coli α-Select competent cells were obtained from Bioline Reagents Ltd (UK) ...
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bioRxiv - Biochemistry 2022Quote: ... coli α-select chemically competent cells (Bioline BIO-85026) and colonies were selected on YT glucose plus 50 mg/mL spectinomycin HCl (Sigma-Aldrich S9007) ...
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bioRxiv - Microbiology 2019Quote: ... coli (alpha-select competent cells, Bioline or XL1-Blue, Stratagene) and plasmids were purified using Zyppy Plasmid Kits (Zymo Research Corporation) ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RNA was converted to cDNA using the SensiFAST cDNA kit (#BIO-65053, BioLine) with 10 μL of RNA extract per reaction following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... or GST were transfected in BL21 (DE3) Escherichia coli bacteria (Bioline). Bacterial starter culture was made by inoculation of 4 mL Luria broth with 10 μg/mL ampicillin ...
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bioRxiv - Molecular Biology 2020Quote: ... coli as per manufacturer’s protocol (α-Select Competent Cells, Bioline, Australia). The transformed cells were grown overnight (37° ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant proteins were produced in Escherichia coli BL21 (DE3) cells (Bioline) as described previously (LaCourse ...
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bioRxiv - Plant Biology 2019Quote: ... and transformed into Escherichia coli α-select chemically competent cells (Bioline) via heat shock ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (TOP10- or Stbl3) using Isolate II plasmid miniprep kit (Bioline).
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bioRxiv - Microbiology 2020Quote: ... coli were extracted using the ISOLATE II Plasmid Mini kit (Bioline, Australia) according to manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... Protein overexpression was performed using Escherichia coli BL21 (DE3) pLysS cells (BIOLINE) in super broth supplemented with 200 μg/mL ampicillin (AMRESCO ...
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bioRxiv - Microbiology 2020Quote: ... cDNA synthesis was performed with 0.25-1 µg of RNA in a 20 µL total reaction volume using a random hexamer/oligo dT strand synthesis kit as per the manufacturer’s instructions (10 min at 25°C; 15 min at 42°C; 15 min at 48°C; SensiFast, Bioline). All oligonucleotide sequences are listed in Table 1.
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bioRxiv - Microbiology 2020Quote: ... and cDNA synthesized with 0.25-1μg of RNA in a 20μL total reaction volume using a random hexamer/oligo dT strand synthesis kit in accordance with the manufacturer’s instructions (10 minutes at 25°C; 15 minutes at 42°C; 15 minutes at 48°C; SensiFast, Bioline). PCR amplification of HBV RNAs were performed using primers as previously described43 using a SYBR green real-time PCR protocol (qPCRBIO SyGreen ...
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bioRxiv - Cell Biology 2021Quote: ... and E-gene expression assessed using the SensiFAST Probe No-Rox One Step Kit (Bioline) and the following primers/probes ...
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bioRxiv - Bioengineering 2022Quote: ... E-gene expression was determined using the SensiFAST Probe No-Rox One Step Kit (Bioline) and the following primers/probes ...
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bioRxiv - Bioengineering 2019Quote: ... coli DH5α was used as the cloning strain and performed transformations according to the manufacturer’s instructions (BIOLINE). E ...
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bioRxiv - Cell Biology 2020Quote: ... PDZ4 (1099–1203)) as well as Scribble mutants were expressed using Escherichia coli BL21 (DE3) pLysS cells (BIOLINE) as Glutathione S-transferase or Maltose Binding Protein fusions (Rautureau et al. ...
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bioRxiv - Microbiology 2023Quote: PCR products for the generation of different potential RNase E targets were amplified with MyTaq™ Red Mix 2x (Bioline), and Synechocystis genomic DNA as template (primers T01 to T12) ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated from lung homogenates from mice exposed to E-cigarettes (with and without nicotine) and room air (SHAM) using Isolate II RNA Mini Kit (Bioline) and cDNA was synthesized using SensiFAST cDNA Synthesis kit (Bioline) ...
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bioRxiv - Genomics 2023Quote: ... 1’ 72°C) by MyTaq Red mix (Bioline) using primers that add overhangs with EcoRI (MT024 ...
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bioRxiv - Genomics 2020Quote: ... at 37°C for 30 min and then proteinase K digestion (Bioline, cat. #37084) for 2 h at 56 °C ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour at 37°C and proteinase K (40 ug, Bioline BIO-37084) for 2 hours at 55°C ...
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bioRxiv - Genomics 2019Quote: ... pool C was diluted 1,000-fold and 1.5 fmol were amplified using VELOCITY DNA polymerase (Bioline) with forward primer pool-FP1 and reverse primer pool-RP2 using the following PCR conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed using SensiMix SYBR Low-ROX Kit (Bioline; annealing temperature – 60°C) in a Lightcycler 480 384-well plate (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cross-linking was reversed by overnight incubation at 60°C with proteinase K (Bioline, Lot # BIO-37037). Then 3C libraries were purified by phenol-chloroform followed by chloroform extraction and ethanol-precipitated at −80°C overnight ...
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bioRxiv - Microbiology 2019Quote: ... for 14 hours at 37 °C in the presence of RiboSafe RNAse Inhibitor (Bioline, cat. number BIO-65028). Next ...
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bioRxiv - Genetics 2021Quote: ... and then digested with proteinase K (20 μg per sample at 56°C for 2 h; Bioline, cat. # 37084), and electrophoresed on a 1.5% agarose gel to verify that the majority of fragments were 100-400 bp in size ...
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bioRxiv - Genetics 2019Quote: ... cDNA was diluted in nuclease-free water and stored at −20°C until used in qPCR with the SensiFastTM SYBR® No-ROX kit (Bioline) and a Lightcycler® 480/1536 (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue pieces were digested at 4 °C on an orbital shaker-incubator at 200 × rpm (Bioline Global, New South Wales, Australia). After overnight digestion ...
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bioRxiv - Microbiology 2020Quote: Bacterial cultures were grown in Luria Bertani (LB) broth for 18-24h at 37 °C and DNA was extracted using the Isolate II Genomic DNA Kit (Bioline, UK) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... then incubated overnight at 37°C with staining buffer (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside [X-gal, Bioline, London ...
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bioRxiv - Cell Biology 2019Quote: ... The hPL-PIPC units were distributed into 45 mL aliquots and stored at −20°C in a freezer (Gram BioLine, Vojens, Denmark). In addition ...
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bioRxiv - Physiology 2021Quote: ... was performed with three primers (Figure 1 A, “a”: AAGGCGCATAACGATACCAC, “b”: TACAATGCAGGCTCCAAACAC, “c”: CGAGCACAGGAAGTTCAACA, Eurofins Genomics, Ebersberg/Germany) and the ImmoMix™ kit (Bioline, Cat. No BIO-25020) according to the manufacturer’s instructions.