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Citations for GE Life Sciences :
1 - 34 of 34 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... plus ATP (100 μM) (GE healthcare). After mixing ...
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bioRxiv - Cell Biology 2023Quote: ... and 6,000 Ci/mmol γ-[32P] ATP (GE Healthcare). Reactions were quenched by the addition of Laemmli sample buffer and analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Cancer Biology 2020Quote: ... 1 mM ATP and 0.25 µM dCTP-Cy3 (GE healthcare). The mixtures were then applied to biochips functionalized with a series of plasmids containing specific lesions ...
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bioRxiv - Biochemistry 2021Quote: ... NTPs (ATP, GTP, CTP, and UTP) were purchased from GE Healthcare Life Sciences.
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bioRxiv - Biochemistry 2022Quote: ... Excess ATP was removed using Microspin G-25 columns (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... 10 μM ATP) and bound to a HiTrapQ column (GE Healthcare). The proteins were eluted with a NaCl gradient (0.01 to 1 M) ...
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bioRxiv - Microbiology 2024Quote: ... 2 µMnt ssDNA (Ovio 45) and 2 mM ATP (GE Healthcare) in a 40 µL reaction solution containing 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 8.0 and 0.2 mM EDTA and analyzed by fluorescein scanning (Typhoon; GE Healthcare, Inc.).
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bioRxiv - Molecular Biology 2023Quote: ... Unbound [γ-32P]-ATP was removed by MicroSpin G-25 columns (GE Healthcare). The RNA was separated by electrophoresis in a denaturing 18 % polyacrylamide gel ...
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bioRxiv - Cell Biology 2020Quote: ... 0.2mM ATP) and subjected to gel filtration chromatography using a Superdex200 column (GE Healthcare) to remove small filaments ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... After removing excessive ATP with illustra™ MicroSpin™ G-25 Columns (GE healthcare), the protein was added to an extra vial containing 10 μM of the phosphate acceptor constructs (PKH497QR1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Unincorporated ATP was removed by Illustra MicroSpin G-25 Columns (GE Healthcare Life Sciences, UK). The radiolabeled RNA probe was denatured in buffer (50 mM Tris-Cl ...
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bioRxiv - Biophysics 2021Quote: ... The MtaLonA-Ig2-MMH8709-ATP-γ-S complex was then loaded onto Superdex 200 (GE Healthcare) column pre-equilibrated with 20 mM Tris-HCl (pH8.0) ...
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bioRxiv - Biophysics 2021Quote: ... Excess P32 gamma-ATP was then separated from labeled AAF DNA by G50 spin column (GE Healthcare). Labeled AAF DNA was mixed 1:4 with unlabeled AAF DNA prior to use for electromobility shift assays (EMSA).
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bioRxiv - Biochemistry 2021Quote: ... ubiquitylation of fluorescein-labelled substrates was visualized with a fluorescent scan of an SDS-PAGE gel with a Typhoon imager (GE Healthcare) and quantified with ImageQuant (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... the labelled oligonucleotides were separated from free [γ-32P] ATP using a custom Sephadex G-25 column (GE Healthcare). Labelled nucleic acids were incubated with nucleases (Rnase A ...
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bioRxiv - Biochemistry 2022Quote: ... and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0, 1M NaCl, 2mM MgCl2, 1mM ATP, 2mM beta-mercaptoethanol) on an ÅKTA system (GE Healthcare). Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Developmental Biology 2020Quote: Three millilitres of ATP-devoid interphase extracts were loaded into a Hiload 16/60 Superdex 200 (GE Healthcare, Life Science). Proteins were eluted with a Tris 50 mM pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Probes were end-labelled using [gamma-32P]-ATP and purified using an Illustra ProbeQuant G-50 micro column (GE Healthcare). A mixture of two different probes was used per target ...
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bioRxiv - Plant Biology 2024Quote: ... Labelled probes were separated from unincorporated γ32P-ATP by gel filtration using G-25 MicroSpin Columns (GE Healthcare Life Sciences). The U6 probe was end-labelled the same way.
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bioRxiv - Cell Biology 2021Quote: ... and the signal of γ-32P-ATP was detected using a PhosphorImager scanned by Typhoon 8600 Variable Mode Imager (GE Healthcare). Bands on the autoradiograms correspond to the molecular weight where Coomassie-stained protein migrates (Figure 1-figure supplement 1).
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bioRxiv - Microbiology 2024Quote: ... fully complementary to the fluorescent strand to avoid it re-hybridizing to the quencher strand) and 5 mM ATP (GE Healthcare) in a 100 µL reaction solution containing 10 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Double stranded Oligonucleotides were end-labeled with T4 polynucleotide kinase in the presence of [γ-32P]ATP and purified using G-25 columns (GE Healthcare). To remove secondary structures present in them ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were dried for one hour and the signal of γ-32P-ATP was detected via a PhosphorImager scanned by Typhoon 8600 Variable Mode Imager (GE Healthcare). For Fig ...
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bioRxiv - Biochemistry 2021Quote: ... Subsequently the gel was washed several times with water to remove residual γ-32P-labelled ATP and phosphoproteins were detected using autoradiography and a Amersham Typhoon RGB biomolecular imager (GE Healthcare). Phosphoprotein signal (P-UPF1 or P-mTORΔN ...
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bioRxiv - Genomics 2021Quote: ... G-25 sephadex™ G-50 DNA grade resin columns were used to remove unincorporated γ-32P ATP according to manufacturer’s instructions (illustra™ GE Healthcare). The purified strand was used for subsequent annealing with equal amount of complementary strand in 50 μL total volume ...
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bioRxiv - Biochemistry 2024Quote: ... at 42°C with 5 µL of γ 32P-ATP end-labeled oligodeoxyribonucleotides and visualized after one day of exposure on a phosphorimager (Typhoon FLA 7000, GE Healthcare). Oligonucleotide KW-pr-610 was used to generate the blot analysis in Fig ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
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bioRxiv - Genomics 2021Quote: ... were 5’ end-labelled with [y-P32]-ATP by T4 polynucleotide kinase (New England Biosciences) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2023Quote: ... The 35 mer DNA oligonucleotide probes were radiolabelled with 32P-ATP at 37°C for 1 hour and then purified over a G50 size exclusion column (GE Healthcare Life Sciences) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... For transphosphorylation assays the kinase was autophosphorylated as described before and excessive ATP was removed using illustra™ MicroSpin™ G-25 Columns (GE healthcare). Afterwards the phosphate receptor protein (10 µM ...
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bioRxiv - Microbiology 2021Quote: ... Primers were labelled using T4 PNK in buffer A and [γ-P32]-ATP (10 mCi/mL, 3,000 Ci/mmol) and purified using G-50 Microspin columns (GE Healthcare, #27-5330-01). Reverse transcription reactions were stopped by adding 40 μL of cold 0.3 M Na-acetate ...
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The LacO-specific probe was end labeled with 32P-γ-ATP for 1 h at 37 °C before cleaning with illustra ProbeQuant G-50 micro column (GE Healthcare; 28-9034-08). The blot was incubated for 2 h at 42 °C in hybridization buffer (ULTRAhyb™ Ultrasensitive Hybridization Buffer [Invitrogen ...