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Citations for GE Life Sciences :
1 - 48 of 48 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... VLCAD (and mutant) and ECSITCTER (and mutants) were purified on Superdex 200 10/300 GL SEC column (GE Healthcare), equilibrated in 25 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant containing toxin was passed through Ni-affinity column (HisTrap FastFlow Crude, GE Healthcare) initially ...
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bioRxiv - Microbiology 2022Quote: ... toxins were purified through Ni2+-affinity chromatography using a HiTrap Heparin HP column (GE Healthcare). Protein capture was verified via visualization of a single band on SDS-PAGE following Coomassie blue staining ...
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bioRxiv - Microbiology 2022Quote: ... control or mutant trophozoites were rinsed once in PBS (GE Life Sciences) and twice in serum free TYI-S-33 medium (SFM) ...
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bioRxiv - Biophysics 2019Quote: ... The mutant histone was purified and labeled with Cy3 maleimide (GE Healthcare) under denaturing conditions (Harada et al. ...
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... Mutant TTR were purified by Ni-affinity chromatography using HisTrap columns (GE Healthcare), followed by gel filtration chromatography using a HiLoad 16/60 Superdex 75 column (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... and ScGrc3 mutants were purified on a HitTrap Q HP column (GE Healthcare). All proteins were further purified by size-exclusion chromatography (Superdex 200 Increase 10/300 ...
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bioRxiv - Genetics 2020Quote: ... rrp1+ and rrp1-CS mutant version were introduced into the pGEX vector(GE Healthcare) by In-Fusion® cloning (Takara Bio) ...
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bioRxiv - Biochemistry 2020Quote: ... except that the DnaBKAEA double mutant was purified using sequential Ni-Sepharose (GE Healthcare) and amylose (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... The K48C mutant of ubiquitin was desalted (HiPrep 26/10 Desalting column, GE Healthcare), and mixed with BFA at a molar ratio of 1:10 in 20 mM pH 7.4 HEPES buffer ...
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bioRxiv - Biophysics 2023Quote: ... The GST-ScLas1 mutants were further purified on a Heparin HP column (GE Healthcare) and ScGrc3 mutants were purified on a HitTrap Q HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... coli BW25113 and the single-gene knockout mutants Δpgm were purchased from Dharmacon (GE Healthcare) and stored in 50% glycerol stock at -80°C ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant WT and mutant INs were analyzed on Superdex 200 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2020Quote: ... PSG or mutants were concentrated and further purified using size exclusion chromatography (S-100, GE Healthcare). Protein purity was quantified by SDS-PAGE and identity by MALDI-TOF mass spectrometry ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-ROC WT and GST-ROC K1347A mutant protein bound to Glutathione Sepharose 4B beads (GE Healthcare) were washed twice with Buffer A (20 mm Tris–HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... WT and mutant DNA gyrases were further purified by gel filtration chromatography (ÄKTA pure, GE Healthcare Bioscience) using a Hi-Load 16/600 Superdex 200 prep grade column (GE Healthcare Bioscience ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant WT and mutant IN molecules were analyzed on Superdex 200 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Cancer Biology 2022Quote: ... WT and mutant Ankrd26 N-Ank modules were generated by cloning into pGEX-6P-1 (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The PhuS or PhuS H212R mutant lysate was applied to a Sepharose-G column (GE Life Sciences) equilibrated with 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: Recombinant WT and mutant IN proteins were analyzed using Superdex 200 10/300 GL column (GE Healthcare) with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2021Quote: ... The amplified wild type and mutant PDE12 cDNAs were cloned into plasmid pGEX-6P-1 (GE Healthcare, USA) at the EcoRI and SalI restriction site ...
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bioRxiv - Cell Biology 2021Quote: ... GST-Bro1V fusion protein and mutants thereof were purified using the Glutathione Sepharose 4B (GE Healthcare, United Kingdom), treated with AcTEV protease (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Multimeric state of WT and mutant INs were analyzed on Superdex 200 10/300 GL column (GE Healthcare) equilibrated with running buffer at a flow rate of 0.2 ml/min.
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bioRxiv - Biophysics 2024Quote: ... The truncated Abl mutants did not bind to either ion exchange Q- or S-sepharose HP (GE Healthcare) columns in ion exchange buffer ...
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bioRxiv - Genetics 2022Quote: ... Mutant and wild-type DASH complexes were first purified via affinity chromatography using either FLAG resin (GE Healthcare Bioscience) or Nickel resin (Bio-Rad) ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: Filtered soluble recombinant wild-type RBPMS-A and mutants were purified on an AKTA protein purification system (GE Healthcare;). Recombinant protein was first purified on HisTrap HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... and ∼ 4 mL of this concentrated ABHD14B mutant was loaded onto a pre-equilibrated HiLoadTM16/60 Superdex200 (GE Healthcare) gel filtration column ...
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bioRxiv - Biochemistry 2021Quote: Single cysteine mutants of ColE9 or the ColE9 DNase domain were desalted on a 5 ml HiTrap column (GE Healthcare) equilibrated with binding buffer (20 mM potassium phosphate pH 7 ...
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bioRxiv - Microbiology 2019Quote: ... fumigatus pksP mutant conidia with an MOI of 10 or 5 in HBSS with Ca2+ and Mg2+ (HyClone, GE Healthcare) on a linear shaker (100 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... Mutants were expressed in BL21 as a standard procedure and then purified with glutathione-sepharose (GE Healthcare, 17-0756-01) as previously described [13] ...
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bioRxiv - Biochemistry 2023Quote: ... GST-ICD or ICD-GST (and alanine-scan mutants) and GST-mEGFP were purified through Glutathione Sepharose beads (GE Healthcare), followed by cation exchange chromatography using a Source 15S column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Candidate mutants defective in vacuolar re-localization of Ost4-GFP were further evaluated using a DeltaVision PersonalDV system (Applied Precision). Strains whose phenotypes were confirmed by reexamination were backcrossed ...
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bioRxiv - Cell Biology 2019Quote: ... Dendra2 was fused to HHO1 or CBF1 in the lys2 deletion mutant from the barcoded YSC1053_KO deletion library (GE Healthcare/dharmacon) (MATa ura3D leu2D his3D1 met15D lys2D::KanR).
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bioRxiv - Developmental Biology 2019Quote: ... Size-exclusion chromatography was performed for the final purification of wild-type and mutant dP75 PWWP using Superdex G75 Hiload 16/60 column (GE Healthcare). Fractions containing target proteins were pooled and concentrated to 10 mg/ml in a buffer containing 10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... REC3 domain (WT and R691A mutant) were further purified by size-exclusion chromatography on a Superdex 200 16/60 column (GE Healthcare) in 20 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... The eluted mutant was then applied to a Superdex 200 10/300 GL size-exclusion chromatography column (Cytiva, Tokyo, Japan (GE Healthcare)) to isolate the monodisperse fraction of the AaRseP mutant ...
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bioRxiv - Microbiology 2022Quote: GF of HisTag-RecAWT and mutants after Nickel-Sepharose columns affinity chromatography continued by size-exclusion chromatography using an ÄKTA FPLC apparatus (GE Healthcare) and a gel filtration column (Superdex 75 16/60 GL ...
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bioRxiv - Biophysics 2019Quote: ... The DTT in the buffer for cysteine mutant of K48-diUb was removed by desalting (HiPrep 26/10 Desalting column, GE Healthcare), and the protein was reacted with the pre-mixed excess Alexa488 and Cy5 dyes ...
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bioRxiv - Neuroscience 2020Quote: SEC-MALS experiments of 100 μM WT and all mutants were carried out by using a Superdex 200 10/300 column (GE Healthcare) in SEC buffer ...
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bioRxiv - Cell Biology 2020Quote: Frozen aliquots of SARS-CoV-2 Spike trimer (Wuhan) and D614G mutant S trimer were thawed and purified by size exclusion chromatography (Superose 6 Increase 10/300, GE Healthcare) using 2 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... and deletion mutant preps were first diluted tenfold with chromatography buffer before being loaded through a Hi Trap Q XL column (GE Healthcare). A linear gradient of sodium chloride from 0 to 1 M was used to purify the chaperonins where each eluted at a conductivity of 5.5 mS/cm ...
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bioRxiv - Molecular Biology 2022Quote: GST-fused bacterial expression plasmid for PAX9 was created by subcloning wild type and mutant (c.336C>G) PAX9 in BamHI and SalI site of pGEX4T1 vector (GE Healthcare, USA). E ...
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bioRxiv - Biochemistry 2023Quote: Oligomerization of ClpL WT and mutants was investigated by size exclusion chromatography (SEC) using a Superose® 6 10/300 GL column (GE Healthcare) at 4°C in assay buffer supplemented with 2 mM ATP ...
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bioRxiv - Immunology 2021Quote: The affinity measurements of hemin towards wild type (hCD36ecto) or mutants (R292A, D372A and Q382A) were performed in MicroCal iTC200 isothermal titration calorimeter (GE Healthcare, Chicago, USA). Unless specified ...
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bioRxiv - Biochemistry 2019Quote: ... OTUB1 and its catalytic mutant (C91S) were further purified by gel filtration chromatography on a HiLoad 16/60 Superdex 75 (GE Healthcare Life Sciences) column equilibrated in 25 mM HEPES pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... DCS experiments with working buffer solutions containing 4 μM of an SBD-mutant and 5 mM substrate were conducted on a VP-DSC Calorimeter (Micro Cal™, GE Healthcare, Buckinghamshire). The melting temperature Tm was determined by ORIGIN 8 (Origin Lab Corp ...
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bioRxiv - Molecular Biology 2021Quote: ... Then 6X-His-tagged wild type SufB precursor and mutant proteins were purified by Ni-NTA affinity column (Ni-NTA His trap, HP GE Healthcare Life Sciences-17524802) (23 ...