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Citations for GE Life Sciences :
301 - 350 of 577 citations for 3 Acetyl N ethylpyridinium bromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Digested DNA was separated by electrophoresis on a 0.8 % agarose gel and then transferred onto Hybond N+ membrane (GE Healthcare, UK). Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG ...
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bioRxiv - Physiology 2021Quote: 200 μl of pooled plasma from gender and genotype matched mice (n = 4-9) was loaded onto a Superose 6 column (GE Healthcare) calibrated with elution buffer (0.15 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 3 µg of RNA was loaded per well on a denaturing agarose gel and was blotted via capillary blot to Hybond(tm)-N+ membrane (GE Healthcare). The membranes were probed with [α32P]UTP-labeled transcripts generated using the MAXIscript® T7 In Vitro Transcription Kit (Ambion ...
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bioRxiv - Biochemistry 2021Quote: ... equipped with an Olympus UPIanApo 100× numerical aperture 1.35 oil objective with the complementing immersion oil (N=1.516, Applied Precision, Issaquah, WA), Standard DeltaVision filters FITC and Rhodamine ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... of RNA was resolved on a 1% agarose denaturing gel followed by blotting on a nylon membrane (Amersham Hybond-N+, GE Healthcare). A probe for mCherry or for 4× Broccoli was synthesized by PCR using the 4× Broccoli plasmid as template and the primers prW150-prW151 for Broccoli and prW459-prW460 for mCherry ...
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bioRxiv - Genomics 2023Quote: ... The digested DNA fragments were separated by electrophoresis using 1% agarose gels and transferred overnight to Hybond-N+ membranes (GE Healthcare) in 20 × SSC ...
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... 45 μl was spotted to a double-membrane system on a dot-blot apparatus with an upper nitrocellulose membrane and a lower hybond-N+ membrane (GE Healthcare). After filtration under vacuum ...
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bioRxiv - Microbiology 2022Quote: ... 6 μg of total RNAs were resolved on a 1 % (w/v) agarose gel then transferred onto Amersham Hybond-N+ Membrane (GE Healthcare) by capillary transfer ...
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bioRxiv - Biochemistry 2022Quote: ... was separated on a denaturing formaldehyde/formamide 1.2 % agarose gel and transferred and UV-crosslinked onto Amersham Hybond™-N membrane (GE Healthcare). RNA was visualized using [32P]-radiolabeled probes targeting mitochondrial RNAs as indicated (see Supplementary Table S1).
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bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
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bioRxiv - Microbiology 2022Quote: ... The N- and C-terminal domains were separated by size exclusion chromatography using HiLoad 16/600 Superdex 200 column (GE Healthcare) in AKTA pure chromatography system (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: The GSTs contained an N-terminal hexahistidine tag enabling their purification by immobilized metal affinity chromatography (IMAC) using a Ni-IMAC column (GE Healthcare). The supernatant fraction from the centrifugation was loaded onto the column equilibrated with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... followed by removal of the N-terminal His6-Ztag by TEV protease cleavage and size exclusion chromatography using a Superdex 200 16/60 column (GE Healthcare) (Roostalu et al ...
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bioRxiv - Molecular Biology 2024Quote: ... or PIG-B (53,54) and fractionated on 10-12% polyacrylamide gel electrophoresis (PAGE)-urea gel and transferred to Amersham Hybond-N+ membrane (GE Healthcare). Transferred membranes were crosslinked 2x with a UV crosslinker (UVP ...
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bioRxiv - Microbiology 2024Quote: ... the DNA molecules that were resolved on the gel were transferred on to the nylon membrane (Amersham Hybond N+, GE Healthcare) by capillary action ...
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bioRxiv - Molecular Biology 2024Quote: An appropriate amount (0.5 – 2 µg) of total RNA was separated in 1% MOPS-formaldehyde agarose gels and transferred to Hybond-N + membranes (GE Healthcare). Membranes were exposed to either randomly [32P]-labelled dsDNA probes ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µg of isolated genomic DNA were digested with SacII and resulting fragments were separated by size on an 0.8 % agarose gel and transferred to a nylon membrane Hybond™-N+ (GE Healthcare) via capillary blot ...
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bioRxiv - Molecular Biology 2021Quote: ... or 8 % denaturing polyacrylamide gel (PAGE) and then transferred to a nylon membrane (Biodyne® A, PALL or Hybond-N+, GE Healthcare). After transfer ...
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bioRxiv - Genomics 2021Quote: ... Neisseria sicca and Neisseria elongata was migrated on a 6% polyacrylamide gel and then transferred onto nitrocellulose membrane (Amersham Hybond™ N+ from GE healthcare). The oligonucleotides were labeled in 5’ by using 5 pmoles of oligonucleotide ...
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bioRxiv - Genetics 2020Quote: Incorporation of [α-32P]-dCTP was measured by spotting 5 μl aliquots of the reaction mixture (after the indicated time points at 37°C) on Hybond N+ membrane strips (GE Healthcare Lifesciences). The membranes were washed (3 × with 2 × SSC and 1 × with 95% EtOH ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were sown on the devices and the devices were put on the Hybond-N+ nylon membrane (GE Healthcare, Chicago, IL, USA) which was placed on 1/2 MS medium supplemented with 0.5% sucrose and 1% agar ...
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bioRxiv - Plant Biology 2024Quote: ... Enrichment of N-terminal labeled peptides was carried out using 200 µL NHS-Sepharose slurry (17-0906-01, GE Healthcare, Solingen, Germany) per 100 µg protein ...
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bioRxiv - Cancer Biology 2024Quote: ... Enrichment of cell surface exposed N-glycopeptides was performed as follows: Peptides were mixed with 100 µl of Streptavidin Sepharose™ High Performance beads (GE HealthCare) in 100 mM NH4HCO3 ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3 mM TCEP and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~10 mg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... or pH 3–10 for CDK2 (Amersham Biosciences, GE Healthcare Europe, Diegem, Belgium) before separation by SDS-PAGE (42 ...
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bioRxiv - Cell Biology 2022Quote: ... HUVEC were coated onto cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) and embedded in a fibrin matrix by combining 7µl of 50U/ml thrombin (T7201-500UN ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm pore-size polycarbonate filters (Whatman, GE Healthcare Europe GmbH, Milan, Italy) previously pre-coated with 2 μg/mL of fibronectin ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer with imidazole concertation brought up to 50 mM and suspension was applied on Flex-Column (420400-2510 ...
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bioRxiv - Cell Biology 2023Quote: ... in the 24-fraction setup using pI 3-10 IPG strips (GE Healthcare) for 50 kVh ...
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bioRxiv - Immunology 2022Quote: ... and 3 mL of 66% percoll was underlaid (GE healthcare 17–0891–01). Samples were spun for 20 min at 2000 rpm at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... HUVEC were attached to dextran-coated Cytodex 3 beads (GE Healthcare Bio-Sciences) and embedded at 250 beads per well in a 24-well plate in a fibrin clot ...
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bioRxiv - Biochemistry 2024Quote: ... 3 mM NaN3) on a Superdex 200 Increase 5/150 column (GE Healthcare) equilibrated by the same buffer and run at a 0.45 ml/min flow rate ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were sown in a prescribed seed pocket of the chip and the chips were put on the Hybond-N+ nylon membrane (GE Healthcare, Chicago, USA) which was placed on the 1/2 MS medium containing 0.5% sucrose and 1% agar ...
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bioRxiv - Genetics 2021Quote: ... All digested DNA was separated by electrophoresis on a 1% TBE agarose gel and subsequently transferred by capillary transfer to an Amersham Hybond-N+ nylon membrane (GE Healthcare, Milwaukee, WI).
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bioRxiv - Biochemistry 2021Quote: ... Selected fractions containing the N protein were concentrated and loaded on two connected Superdex 200 10/300 GL size exclusion columns (GE Healthcare Life Sciences) pre-equilibrated with 20 mM HEPES ...
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Stress response in Entamoeba histolytica is associated with robust processing of tRNA to tRNA-halvesbioRxiv - Microbiology 2021Quote: ... 20 μg RNA samples were separated on a denaturing 12% polyacrylamide gel and transferred to a membrane (Amersham™ Hybond™ -N+ Membrane, GE Healthcare). Probe DNA was 5′-end labeled by PNK reaction using γ-[32P]-ATP and hybridized with the membrane in perfectHyb buffer (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
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bioRxiv - Molecular Biology 2022Quote: ... Gels were blot-transferred to a nylon membrane (Amersham Hybond-N+ #RPN203B) and probed with pBR322 DNA sequences labelled with AlkPhos Direct (GE Healthcare® #GERPN3680). Chemiluminescent signals of increasing exposition periods were recorded with a cooled CCD camera (KODAK Gel Logic 1500 Imaging System ...
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bioRxiv - Biophysics 2022Quote: ... was titrated into an A1 protein solution (22 μM except 50 μM with A1+OGly+N+C) in a MicroCal iTC200 (GE Healthcare Life Sciences). A priming injection of 0.4 μL (not included in data analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA in the gel was transferred overnight to a nitrocellulose membrane with a positive charge (GE healthcare Amersham™ Hybond™ -N+) by capillary transfer using an assembly of Whatman® filter paper ...
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was purified away from SenP1 and the N-His6-SUMO-tag by FPLC on a Mono Q 10/100 GL anion exchange column (GE Healthcare Life Sciences) using buffers containing 50 mM EPPS (pH 8.5) ...
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bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged Cg1978-C-His to GyrA-N-Strep was analyzed by SPR analysis in a Biacore 3000 device (GE Healthcare, Freiburg, Germany) using a Sensor Chip CM5 (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: [18F]FTHA was synthesized using the method from DeGrado 48 with modifications on a modified TRACERlab FXF-N synthesizer (GE Healthcare, Münster, Germany). Briefly ...