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Citations for GE Life Sciences :
201 - 250 of 3682 citations for N 5 Trimethoxysilyl 2 Aza 1 Oxopentyl Caprolactam since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... the gel was photographed and RNA was transferred to Hybond-N+ (GE Healthcare) by standard capillary transfer.
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... Electroblotting of the RNA onto a Hybond-N+ membrane (GE Healthcare Life Sciences) was performed at 50 V ...
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bioRxiv - Plant Biology 2023Quote: ... the RNA was blotted onto the HybondTM-N+ nylon transfer membrane (GE Healthcare) by soaking in 20× SSC (3.0 M NaCl and 0.3 M sodium citrate ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA samples were loaded onto an Amersham Hybond-N+ membrane (RPN119B, GE Healthcare) using a Bio-Dot apparatus (#170-6545 ...
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal His6 tag was removed by digestion with thrombin (GE Healthcare) by adding the enzyme at a concentration of 10 Units per mg of KsgA protein during overnight dialysis against PBS ...
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bioRxiv - Genetics 2022Quote: ... Digested DNA was transferred by capillarity to a Hybond-N membrane (GE Healthcare) and hybridized with 32P-labeled probe which is specific to CaLCuV DNAA ...
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bioRxiv - Biophysics 2023Quote: ... N protein was then bound to an SP sepharose FF column (GE Healthcare) and eluted using a gradient of 0-100% buffer B (buffer A ...
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bioRxiv - Biophysics 2023Quote: ... N protein was then bound to an SP sepharose FF column (GE Healthcare) and eluted using a gradient of 0-100% buffer B (buffer A ...
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bioRxiv - Biophysics 2023Quote: ... The N protein was then run over a HisTrap FF column (GE Healthcare) in Buffer A (20 mM Tris pH 7 ...
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bioRxiv - Microbiology 2024Quote: ... RNAs were then transferred on Hybond N+ nitrocellulose membrane (GE Healthcare Life Sciences). Membranes were hybridized with specific digoxygenin (DIG)-labelled probes targeting RNA of interest or 5S rRNA (loading control) ...
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bioRxiv - Cell Biology 2024Quote: ... The RNA was transferred overnight to a Hybond-N+ nylon membrane (GE Healthcare) by upward capillary transfer with 20x SSC buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The extracted mRNA was denatured and spotted onto Hybond N+ membrane (GE Healthcare), then cross-linked using a Stratalinker UV Crosslinker 2400 (Stratagene) ...
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bioRxiv - Microbiology 2022Quote: ... the respective supernatants were applied to 1/5 ml HiTrap™ Q XL columns (GE Healthcare) for anion exchange chromatography using the ÄKTA Start chromatography system (GE Healthcare ...
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Adaptation of the Romanomermis culicivorax CCA-adding enzyme to miniaturized armless tRNA substratesbioRxiv - Molecular Biology 2020Quote: ... sterile filtrated and loaded onto a HisTrap FF 1 ml or 5 ml column (GE Healthcare). Column wash was performed with 4-10 column volumes of binding buffer (25 mM Tris/HCl pH 7.6 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were further purified using a 1 or 5 ml Hi-trap Q column (GE Healthcare) that was equilibrated in (100 mM NaCl,10 mM KH2PO4 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM TCEP and loaded to 5 ml HiTrap SP HP (GE Healthcare, 17-1152-01). After extensive washing ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were loaded on a 5 ml HisTrap™HP column (1 mL/min; GE Healthcare) in a binding buffer containing 100 mM tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... 5 % glycerol into two 5 mL MBP-trap cartridges (GE Healthcare) connected in tandem before removal of the Ni-NTA cartridges ...
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bioRxiv - Cell Biology 2020Quote: ... The mixture was incubated for 2 hours at RT and antibodies were isolated using illustra™ NAP-5 columns (GE Healthcare Life Sciences™), resulting in an average labeling rate between 0.8 and 1.
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated in Buffer B ...
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bioRxiv - Cell Biology 2020Quote: ... the corresponding cDNAs of mouse angulin-1 were amplified by PCR using KOD-Plus-Ver.2 DNA polymerase and subcloned into pGEX-6P-1 (GE Healthcare). To construct an expression vector for MBP-tagged N-ZO-1 (aa 1-862) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... containing 1 mM 2-ME) and Superdex 200 Increase 10/300 GL column (GE Healthcare; 10 mM KPB [pH 7.0] containing 50 mM NaCl and 1 mM 2-ME) ...
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bioRxiv - Plant Biology 2022Quote: ... The primary antibody was diluted 1:10,000 in TBST-Buffer with 2% Blocking (GE Healthcare) and incubated on the membrane for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP, 5 mL; GE Healthcare) that had been previously equilibrated with Ni-NTA Buffer (NB ...
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bioRxiv - Cell Biology 2020Quote: ... The gel was subsequently electroblotted onto a nylon membrane (Amersham Hybond N+, GE Healthcare) as described 55 ...
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bioRxiv - Molecular Biology 2021Quote: ... The complexes were transferred to an Amersham Hybond-N+ nylon membrane (RPN303B, GE Healthcare), UV-crosslinked ...
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bioRxiv - Molecular Biology 2021Quote: ... we transferred the RNAs from the gel to HybondTM-N+ nylon membrane (GE Healthcare) by capillary transfer O/N and cross-linked the RNA to the membrane by an ultraviolet cross-linker (120 mJ/cm2 at 254 nm ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was afterwards blotted onto Nylon membranes (Amersham Hybond-N+, GE Healthcare, RPN203B) with 10X saline-sodium citrate buffer (SSC ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was transferred overnight to Hybond-N+ nylon membrane (GE Healthcare, Chicago, Illinois, RPN303B) by capillary action in transfer buffer (0.5 M NaOH ...
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bioRxiv - Microbiology 2020Quote: ... Separated RNA was electro-transferred from the gel to Hybond-N+ membranes (GE Healthcare) at 50 V for 1 h at 4°C and the membranes were UV-crosslinked (120 mJ/cm2) ...
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bioRxiv - Bioengineering 2022Quote: ... We purified the N-terminal His-tagged proteins on a HisTrap column (GE Healthcare) and then by size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2020Quote: ... chilled directly on ice and spotted on a nylon membrane (GE Healthcare Hybond-N+). After UV-induced fixation of target nucleic acids (2 x 200 000 μJ/cm2 of UV) ...
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bioRxiv - Biochemistry 2020Quote: ... The RNA were subsequently electrophoretically transferred to Amersham Hybond-N+ nylon membranes (GE Healthcare) and probed with a 32P-labeled DNA oligonucleotide specific for SCR1 (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... The RNA were nanodropped and then spotted on the hybond N+ membrane (GE Healthcare) as previously described (17) ...
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bioRxiv - Cancer Biology 2023Quote: ... Specified amounts of DNA were blotted on a Hybond N+ membrane (GE Healthcare, UK) in a 1μl volume and left to air-dry ...
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bioRxiv - Biochemistry 2023Quote: ... The RNA was then transferred to a HyBond-N+ nylon membrane (GE Life Sciences) using an electrophoretic transfer apparatus (Idea Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was then transferred to Hybond® N+ membranes (cat. no. RPN203B, GE Healthcare) by capillary action using 10X SSC (1.5M NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA solutions were spotted onto Hybond-N+ positively charged nylon membrane (RPN1210B, GE Healthcare). Membranes were blocked at room temperature for 1 hour in TBS-T (Tris-Buffered Saline (TBS) ...
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bioRxiv - Plant Biology 2024Quote: ... 8 μL of each mixture was loaded onto Hybond N nylon membrane (GE Healthcare), which was left being dried out at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was then filtered through a 0.2 µm syringe filter before being loaded onto 2 x 5 mL StrepTrap HP Columns (GE Healthcare Life Sciences 28-9075-47), equilibrated in Step A Buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated at 4°C overnight with primary antibody (1:1000) and incubated for 1 hour at RT with secondary antibodies in either 2% PBA (GE healthcare, RPN418), 5% ELK or OBB (LI-COR ...
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bioRxiv - Cell Biology 2020Quote: ... 2) the reaction mix was purified using anion exchange column (HiTrap QFF 1 mL; GE Healthcare); 3 ...
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bioRxiv - Cancer Biology 2021Quote: ... and after 1 and 2 days of co-culture on an INCell Analyzer 2200 (GE Healthcare), with a large field-of-view sCMOS camera (resolution 2048×2048 pixels ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM β-mercaptoethanol) and applied to a 1-ml Hi-Trap Heparin column (GE Healthcare) equilibrated with TGED ...
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bioRxiv - Neuroscience 2020Quote: The two animals (Monkey 1 and Monkey 2) were scanned on a Discovery MI (GE Healthcare) PET/CT scanner ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times with PBST for 5 min and incubated for 1 h at room temperature with HRP-conjugated secondary anti-rabbit IgG (GE Healthcare, NA934, 1/2000) or anti-mouse IgG (Amersham ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...