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Citations for GE Life Sciences :
2351 - 2400 of 3646 citations for 3 5 Diacetoxy 2 acetoxymethyl 6 phenethyl tetrahydro pyran 4 yl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 mg of 3C protease per 3 L of culture volume and further separated on a Superdex 200 HiLoad column (GE Healthcare) in 50mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl) at approximately 3 mg/mL and then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare) equilibrated in solubilization buffer ...
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bioRxiv - Cell Biology 2020Quote: ... A probe was generated from a PCR fragment homologous to the 3’ sequence just outside of the targeted region using the AlkPhos direct labelling kit (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Full-length coding regions were amplified and inserted in frame into pET-28a(+) (69864-3, MilliporeSigma) and/or pGEX-4T-1 (28-9545-49, GE Healthcare) to create N-terminal ...
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bioRxiv - Microbiology 2020Quote: ... the pirA gene was cloned into XhoI and EcoRI sites of pGEX-4T-3 vector (GE Healthcare Life Sciences, Freiburg, Germany), encoding recombinant PirA with N-terminal-fused GST-tag ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Cell Biology 2023Quote: ... Time-lapse imaging of one-cell embryos expressing CYB-1::mNG or mNG::CYB-3 was performed on a widefield deconvolution microscope (DeltaVision Elite; Applied Precision) equipped with a charge-coupled device camera (pco.edge 5.5 sCMOS ...
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bioRxiv - Microbiology 2023Quote: ... The eluted fractions were concentrated to 3 ml and applied to 120 ml HiLoad 16/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... His6-GB1-tag was subsequently cleaved by TEV protease (3 mM) and separated from bZIP on a cation exchange column (HiTrap SP; GE Healthcare) using a 0–2 M gradient of sodium chloride ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1-Cas2/3 complex was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer C ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was incubated for 3 h at room temperature and loaded onto a Superdex 200 10/300 GL (GE Healthcare) sizeexclusion chromatography column using buffer B as mobile phase ...
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bioRxiv - Genomics 2022Quote: ... Samples were then loaded onto a 1% agarose gel containing 3% formaldehyde and 1x MOPS and run at 75 V in 1x MOPS for ~3 hs after which RNA was transferred onto a Hybond N+ membrane (GE Healthcare) overnight ...
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bioRxiv - Synthetic Biology 2022Quote: ... then wrapped into a clear foil and exposed in the cassette with the phosphor screen for 3 days and visualized on a Phosphorimager (Typhoon FLA 7000, GE Healthcare). prDC1416 was used to generate the blot in Fig ...
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bioRxiv - Biochemistry 2023Quote: SUV420H1-H2A.Z nucleosome complex was assembled in a ratio of 3:1 and was purified on HiLoad Superdex 200 10/300 (GE Healthcare) size-exclusion liquid chromatography column (Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... control HUVEC or HUVEC 48 hr post-siRNA treatment were incubated with cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) overnight then resuspended in 2.2 mg/ml fibrinogen (820224 ...
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bioRxiv - Cell Biology 2024Quote: ... Parasites were synchronized to a 3-h age window by isolating late schizonts from a 60% Percoll (GE Healthcare, Life Sciences) gradient and culturing these for 3 h with fresh erythrocytes (104) ...
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bioRxiv - Developmental Biology 2024Quote: ... a cDNA fragment encoding the C-terminal portion of mouse Ccrk (residues 289-346) was amplified by PCR and subcloned into the pGEX-4T-3 vector (GE Healthcare). The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3 ...
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bioRxiv - Immunology 2024Quote: ... were isolated from blood from healthy volunteers or pwCF mixed 3:1 in warm RPMI and layered over Ficoll-Paque PLUS (GE healthcare). Samples were centrifuged at 450g for 45 minutes and the PBMC layer removed ...
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bioRxiv - Neuroscience 2024Quote: All scans were conducted in a 3 Tesla General Electric scanner with an eight-channel head coil (GE MR750; GE Healthcare). Anatomical images were acquired via T1-weighted ...
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bioRxiv - Cell Biology 2024Quote: ... cleared extracts were alkylated twice with 20 mM iodoacetamide (3 h at RT in the dark) and then cleaned by two passages on PD-SpinTrap G-25 columns (GE Healthcare). Samples were then clicked as explained before ...
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bioRxiv - Cell Biology 2024Quote: Cell or collagen lysates were separated by denaturing SDS-PAGE (6% gels for collagens, 10% for SMADs) and proteins blotted onto a nitrocellulose membrane (Amersham™, GE Healthcare, Chalfont St Giles, United Kingdom). Membranes were cut horizontally to detect proteins of different sizes ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were UV irradiated for 5 minutes and imaged using an Amersham Imager 600 (GE Life Sciences). Gels were then equilibrated in Tris-glycine transfer buffer for 5 minutes and transferred onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). The protein was then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The first purification step was affinity chromatography with a 5 mL HisTrap™ FF column (GE Healthcare) precharged with Ni2+ and equilibrated in buffer A (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... elution from the HisPur™ Cobalt Resin was applied to a 5 ml streptactin column (GE Healthcare) and washed with HBS ...
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bioRxiv - Biochemistry 2020Quote: ... The filtered sample was applied to a 5 mL HisTrap HP column (GE Healthcare, #17-5248-02), pre-equilibrated with Buffer N and 40 mM imidazole using a peristaltic pump ...
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bioRxiv - Biochemistry 2021Quote: ... The target was captured from the cleared lysate on a 5 ml HisTrap FF column (GE Healthcare) and eluted with a linear gradient from 10 to 250 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... followed by hydrophobic interaction chromatography (HIC) using a 5 mL HiTrap Phenyl FF (HS) column (GE Healthcare). For the ion exchange procedure ...
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bioRxiv - Biochemistry 2021Quote: AsChi18A was purified by immobilized metal affinity chromatography using a HisTrap FF 5 mL column (GE Healthcare). The periplasmic extract containing AsChi18A was applied on the column using a binding buffer consisting of 20 mM Tris-HCl pH 8.0 and 5 mM imidazole ...
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bioRxiv - Genomics 2021Quote: ... The diluent was reloaded on AKTA with 5 mL Hitrap Q HP column (GE Healthcare Life Sciences), followed by a wash with 35 mL buffer containing 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... combined and further purified by anion-exchange chromatography using a MonoQ 5/50 GL column (GE Healthcare, buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The FdR9-containing flow-through was loaded on a 5 mL HiTrap Q HP column (GE Healthcare) and elution was done using a linear NaCl gradient (0.04 - 1 M ...
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bioRxiv - Biophysics 2020Quote: ... the mixture of tetramers was purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) using a column gradient from 0.1 to 0.4 M NaCl ...
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bioRxiv - Bioengineering 2020Quote: ... The 0.2 μm filtered cleaved product was injected in a 5 mL HiTrap SP HP (GE Healthcare) cationic exchange column and eluted with a gradient to buffer F (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was loaded into a 5 mL Ni–NTA affinity chromatography column (GE Healthcare, United States) that was pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 300 mM NaCl (IMAC B buffer) and loaded onto a 5 mL HiTrap IMAC column (GE Healthcare) using an Äkta Start Chromatography system ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-free lysate was applied into a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated in start buffer ...
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bioRxiv - Biophysics 2020Quote: ... the solution was loaded on a 5 mL Ni-NTA HiTrap chelating column (GE Healthcare, Chicago IL) that was equilibrated in Ni-NTA base buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The labeled viruses were purified from excess dyes on a Nap-5 gel filtration column (GE Healthcare) that was equilibrated with 50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a HisTrap column (5 mL, cat. No. 17-5248-01, GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... After cell disruption the cleared lysate was loaded on a 5 ml HisTrap FF column (GE Healthcare) equilibrated in lysis buffer A and the protein was eluted with a gradient of 25 – 250 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... followed in tandem by a HiTrap™ Q Sepharose HP column (5 ml/min, GE Life Sciences). The SP Sepharose column was removed before the protein was stepped off from the Q sepharose with Buffer B2 (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
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bioRxiv - Molecular Biology 2020Quote: ... Rubisco-containing fractions were applied to an anion exchange column (MONO Q 5/50 GL, GE Healthcare) and eluted with a linear salt gradient from 30 to 500 mM NaCl in lysis buffer.
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bioRxiv - Microbiology 2020Quote: ... Purification was done using 5 mL pre-packed Ni-sepharose AKTA Hi-TRAP column (GE Healthcare, USA) equilibrated (100 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2021Quote: ... The next morning the cleaved protein was loaded on a 5 ml HiTrap HeparinHP column (GE Healthcare). The protein was eluted over a gradient from 100 mM to 2M KCl ...
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bioRxiv - Biophysics 2021Quote: ... the solution was loaded onto a 5 mL Ni-NTA HiTrap chelating column (GE Healthcare, Chicago IL) that was equilibrated in Ni-NTA base buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... 100 mM NaCl pH 7.0 and further purified on a 5 ml Heparin affinity column (GE Healthcare) with a 100 ml gradient of 0.1 to 1.5 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... and 5 was further purified by gel filtration on a 16/600 Superdex 200 column (GE Healthcare) in S200 buffer ...