Labshake search
Citations for Quantifoil Micro Tools :
1 - 15 of 15 citations for Siglec 15 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 3.5 μl of this solution was adsorbed onto glow-discharged (PELCO EasiGlow, 15 mA, 0.39 mBar, 30 s) carbon-coated grids (200-400 mesh copper, Quantifoil) for 1 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
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bioRxiv - Biophysics 2019Quote: ... 3µl aliquots of assembled ribosomal complexes at 240-390 nM concentration were incubated for 15s either on plasma-treated holey carbon grids (QUANTIFOIL R2/2 with homemade continuous carbon film estimated to be 50Åthick ...
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bioRxiv - Molecular Biology 2022Quote: Three microliter glucagon amyloid fibril sample (see above) was applied onto glow-discharged (15 mA current; negative charge; 60 sec) holey carbon grid (Quantifoil R1.2/1.3 Cu 200 mesh ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µL of each sample was applied to glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold or copper 200 mesh support) ...
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bioRxiv - Molecular Biology 2021Quote: ... After another 3 min at 37 °C the termination complexes were placed on ice for 15 min and subsequently applied to Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools) either coated with a thin continuous carbon film or with graphene oxide flakes (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: 4 μL of C5-OmCI-RaCI-CirpT1 in PBS (0.3 mg/ml) was applied to freshly glow-discharged (20 s, 15 mA) carbon-coated 200-mesh Cu grids (Quantifoil, R1.2/1.3). Following incubation for 10 s ...
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bioRxiv - Microbiology 2019Quote: Cells of strain Pla133T were mixed with the same volume of BSA-stabilized 15 nm colloidal gold solution (Aurion) and placed on holey carbon-coated 200 mesh copper grids (R2/1, Quantifoil, Jena, Germany) immediately before thin-film vitrification by plunge-freezing in liquid propane (63%)/ethane (37% ...
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bioRxiv - Biochemistry 2022Quote: ... 20 mg/ml for the D5R-Rotigotine-Gs complex and 15 mg/ml for the D1R-Rotigotine-Gs complex were applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3). Grids were plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fischer Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... The SEC-purified complex was concentrated using a 50 kDa cut-off concentrator to 1.5 mg/ml and applied to glow-discharged (0.39 mbar, 15 mA, 30 s) holey carbon grids (QuantiFoil R1.2/1.3 Au 200 mesh). Surplus sample was blotted off for 2-4 seconds with zero blotting force in a controlled environment (4 °C ...
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bioRxiv - Biophysics 2021Quote: ... 3 μL of the purified lasmiditan–5-HT1F–Gi complex at concentration ∼15 mg/ml were applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3 Au 300). Grids were plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μL of purified CB1-Gi complex at 15 mg/ml was applied on glow- discharged holey carbon gold grids (Quantifoil R1.2/1.3, 200 mesh). The grids were blotted using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2023Quote: ... A 3 µL drop was applied to a glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold 200 mesh support), blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: Eleven cryoEM grids were prepared by two people by adding 3 μL of mouse apoferritin (mApof, from Dr. Kikkawa’s lab) solution (8 mg/mL) to UltrAuFoil 1.2/1.3 grids (Quantifoil, Jena, Germany) immediately after plasma cleaning (Gatan Solarus II plasma cleaner ...
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bioRxiv - Cell Biology 2022Quote: Tfam+/− MEFs (TFAM Het MEF) and SV40 mouse embryonic fibroblasts (SV40 MEF) were cultured on R ¼ Carbon 200-mesh gold electron microscopy grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture using a Vitrobot Mark 4 (Thermo Fischer Scientific) ...